We narrowed to 34,856 results for: cas

1. CROPseq-EFS-HypaSpCas9-P2A-EGFP

   Plasmid

   #106964

   Purpose

   single-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screen

   Depositor

   Alex Hewitt

   Insert

   HypaSpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. lenti-EF1a-dCas9-KRAB-Puro

   Plasmid

   #99372

   Purpose

   3rd generation lenti vector encoding dCas9-KRAB with 2A puromycin resistance marker (EF1a-dCas9-KRAB-T2A-Puro-WPRE)

   Depositor

   Kristen Brennand

   Article

   Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

   Insert

   dCas9-KRAB-T2A-Puro
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-UbC-VP64-dCas9-VP64

   Plasmid

   #232179

   Purpose

   Lentiviral cassette that expresses 2xVP64-dCas9 fusion in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Rohm et al Cell Genom. 2025 Feb 12;5(2):100770. doi

   Insert

   VP64-dCas9-VP64
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   hUbC

   Available since

   March 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. phU1-CasRx-non-targeting (pKW82)

   Plasmid

   #229050

   Purpose

   Cloning backbone for CasRx crRNA (Human U1 promoter)

   Depositor

   S Chul Kwon

   Article

   Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSpCas9-2A-Puro-TBK1-gRNA (PX459)

   Plasmid

   #221550

   Purpose

   Expresses Cas9 and gRNA for disruption of TBK1 gene in human cells

   Depositor

   Shawn Ferguson

   Article

   Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

   Insert

   gRNA targeting human TBK1 (TBK1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   u6

   Available since

   July 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. FUGW U6 gL1HSg2dCas9-KRAB-T2a-GFP

   Plasmid

   #234881

   Purpose

   L1HS-silencing plasmid (CRISPRi gRNA2)

   Depositor

   Johan Jakobsson

   Article

   Adami et al bioRxiv 2025.01.17.633315

   Insert

   LacZ gRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. FUGW U6 gL1HSg1dCas9-KRAB-T2a-GFP

   Plasmid

   #234882

   Purpose

   L1HS-silencing plasmid (CRISPRi gRNA1)

   Depositor

   Johan Jakobsson

   Article

   Adami et al bioRxiv 2025.01.17.633315

   Insert

   L1HS-silencing plasmid (CRISPRi gRNA1)
   

   Use

   CRISPR and Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. dCas9-DNMT3a-DNMT3l-3xFLAG-tagBFP

   Plasmid

   #154139

   Purpose

   Expresses the dCas9-DNMT3a-DNMT3l-3xFLAG-tagBFP fusion protein for targeted DNA methylation.

   Depositor

   Albert Jeltsch

   Article

   Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i

   Insert

   dCas9, DNMT3a (catalytic domain), DNMT3l (C-terminal part), tagBFP
   

   Use

   Tags

   3x FLAG and tagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. phU1-PspCas13b-non-targeting (pEZ36)

   Plasmid

   #229053

   Purpose

   Cloning backbone for PspCas13b crRNA (Human U1 promoter)

   Depositor

   S Chul Kwon

   Article

   Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAGEN-dPspCas13b-4EHP-NES-mCherry

    Plasmid

    #233610

    Purpose

    Expression of catalytically-inactive PspCas13b fused with 4EHP in mammalian cells

    Depositor

    Shintaro Iwasaki

    Article

    Apostolopoulos et al Nat Commun. 2024 Mar 11;15(1):2205. doi:

    Insert

    dPspCas13b-4EHP-NES-mCherry
    

    Use

    CRISPR

    Tags

    3xFlag and mCherry

    Expression

    Mutation

    H133A/H1058A

    Promoter

    Available since

    March 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pE-35S-miniSdd7-nCas9-PBE-GmPPO2

    Plasmid

    #204855

    Purpose

    For cytosine base editing using miniSdd7 at PPO2 site in soybean (Agrobacterium-mediated transformation)

    Depositor

    Caixia Gao

    Article

    Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

    Insert

    miniSdd7-nSpCas9(D10A)-UGI-mScarlet
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A in SpCas9

    Promoter

    35S, AtU6-26p

    Available since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV-EFS-NLS-CasRx-U6-DR-sgRNA_LacZ-DR

    Plasmid

    #154003

    Purpose

    Vector expressed CasRx and sgRNA_LACZ for AAV package

    Depositor

    Hui Yang

    Article

    He et al Protein Cell. 2020 Mar 18. pii: 10.1007/

    Insert

    CasRx
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    EFS

    Available since

    July 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMSCV-LTR-dCas9-VP64-BFP

    Plasmid

    #46912

    Purpose

    Human expression vector containing MSCV LTR promoter, dCas9 that is fused to 2x NLS, VP64 and tagBFP

    Depositor

    Stanley Qi, Jonathan Weissman

    Article

    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

    Inserts

    dCas9-VP64-BFP fusion
    Puromycin resistance
    

    Use

    CRISPR and Retroviral

    Tags

    3xNLS, BFP, and VP64 domain

    Expression

    Mammalian

    Mutation

    Promoter

    LTR and PGK

    Available since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. Lenti-EF1alpha-dCas9-KRAB-MeCP2_Hygro

    Plasmid

    #192664

    Purpose

    3rd generation lenti vector encoding dCas9-KRAB-MeCP2 with 2A Hygro resistance marker

    Depositor

    Jeffrey Miner

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A, D839A, H840A and N863A in Cas9

    Promoter

    EF1a

    Available since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-CMV-FLEX-SaCas9-U6-sgTh

    Plasmid

    #159901

    Purpose

    Mutagenesis of Th

    Depositor

    Larry Zweifel

    Insert

    Th (Th Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. Px461-Cas9n-Trp53-sgRNA-beta

    Plasmid

    #88847

    Purpose

    CRISPR KO of Trp53

    Depositor

    Joan Massague

    Article

    Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

    Insert

    Trp53 (Trp53 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

17. pZR112_Lenti-SFFV-mCherry-2A-dCas9-VP64

    Plasmid

    #180263

    Purpose

    Lentiviral expression vector for dCas9-VP64 in mammalian cells

    Depositor

    Alexander Marson

    Article

    Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Nuclease dead Cas9

    Promoter

    SFFV

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. Px461-Cas9n-Trp53-sgRNA-alpha

    Plasmid

    #88846

    Purpose

    CRISPR KO of Trp53

    Depositor

    Joan Massague

    Article

    Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

    Insert

    Trp53 (Trp53 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

19. pHR-SFFV-dCas9-BFP-KRAB

    Plasmid

    #46911

    Purpose

    Human expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS, tagBFP and a KRAB domain

    Depositor

    Stanley Qi, Jonathan Weissman

    Article

    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

    Insert

    dCas9-BFP-KRAB fusion
    

    Use

    CRISPR and Lentiviral

    Tags

    2xNLS, BFP, HA, and KRAB domain

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pXR003: CasRx gRNA cloning backbone

    Plasmid

    #109053

    Purpose

    hU6-driven expression of guide RNAs compatible with CasRx. 5' processed DR followed by BbsI sites for guide cloning.

    Depositor

    Patrick Hsu

    Article

    Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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