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AAVS1-Puro CAG CLTX-NKG2D-2B4-CD3z CAR

Plasmid

#157744

Purpose

knock CLTX-NKG2D-2B4-CD3z CAR into AAVS1 safe harbor locus for constitutive expression

Depositor

Xiaoping Bao

Article

Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

Insert

chlorotoxin containing chimeric antigen receptor targeting glioblastoma

Use

CRISPR, Synthetic Biology, and TALEN

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-RET

Plasmid

#116787

Purpose

Lentiviral expression of RET

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

RET (RET Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pGS-FCER1G-1

Plasmid

#109192

Purpose

Encodes human full-length FCER1G to be expressed in a baculovirus/insect cell expression system

Depositor

Monika Golas

Article

Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

Insert

Human full-length FCER1G, codon-optimized (FCER1G Human)

Expression

Insect

Promoter

p10

Available Since

Jan. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FFSS-BACH1

Plasmid

#232273

Purpose

Expression of N-terminal tagged 2xFLAG-2xSTREP-BACH1 (H. sapiens) in human cell lines

Depositor

Michele Pagano

Article

Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

Insert

BACH1 (BACH1 Human)

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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MEK1C222

Plasmid

#164638

Purpose

Bacterial expression plasmid for His6-MEK1C222

Depositor

Ben Davis

Article

Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

Insert

mitogen-activated protein kinase kinase 1 (MAP2K1 Human)

Tags

His6-tag

Expression

Bacterial

Mutation

G(-19)F/S222C/C277S/C376S

Promoter

T7

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CG

Plasmid

#116774

Purpose

Lentiviral expression of PIK3CG

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CG (PIK3CG Human)

Use

Lentiviral

Mutation

WT

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CD-E1021K

Plasmid

#116566

Purpose

Lentiviral expression of PIK3CD E1021K

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CD (PIK3CD Human)

Use

Lentiviral

Mutation

E1021K

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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PL-CRISPR.EFS.tRFP-gIfngr1-1

Plasmid

#166479

Purpose

Expresses spCas9, tRFP and a gRNA targeting mouse Ifngr1

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

gRNA for mouse Ifngr1 (Ifngr1 Mouse)

Use

Lentiviral

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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PL-CRISPR.EFS.tRFP-gIfngr1-2

Plasmid

#166480

Purpose

Expresses spCas9, tRFP and a gRNA targeting mouse Ifngr1

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

gRNA for mouse Ifngr1 (Ifngr1 Mouse)

Use

Lentiviral

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-PPP2R1A-P179R

Plasmid

#116608

Purpose

Lentiviral expression of PPP2R1A P179R

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PPP2R1A (PPP2R1A Human)

Use

Lentiviral

Mutation

P179R

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pUC19-YAP-mEGFP

Plasmid

#166472

Purpose

HDR template for tagging of endogenous mouse YAP N-terminus with mEGFP

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

YAP-mEGFP HDR template(mouse Yap) (Yap1 Mouse)

Expression

Mammalian

Available Since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CB-E1051K

Plasmid

#116553

Purpose

Lentiviral expression of PIK3CB E1051K

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CB (PIK3CB Human)

Use

Lentiviral

Mutation

E1051K

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CG-E267K

Plasmid

#116577

Purpose

Lentiviral expression of PIK3CG E267K

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CG (PIK3CG Human)

Use

Lentiviral

Mutation

E267K

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CB-D1067Y

Plasmid

#116551

Purpose

Lentiviral expression of PIK3CB D1067Y

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CB (PIK3CB Human)

Use

Lentiviral

Mutation

D1067Y

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag2-Sox9 T236A/T240A

Plasmid

#111458

Purpose

Transient expression of Flag-tagged human Sox9 with T236A/T240A mutation

Depositor

Sharon Pine

Article

Hong et al Nucleic Acids Res. 2016 Oct 14;44(18):88

Insert

Sox9 (SOX9 Human)

Tags

Flag

Expression

Mammalian

Mutation

T236A and T240A

Promoter

CMV

Available Since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CB-E554K

Plasmid

#116555

Purpose

Lentiviral expression of PIK3CB E554K

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CB (PIK3CB Human)

Use

Lentiviral

Mutation

E554K

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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mEGFP-N1-YAPS127A-deltaCC

Plasmid

#166459

Purpose

Expresses fusion of mEGFP and YAPS127A-deltaCC (delta298-359)

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP, YAPS127A-deltaCC (YAP1 Human)

Expression

Mammalian

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3R1-S460_D464delSREYD

Plasmid

#116605

Purpose

Lentiviral expression of PIK3R1 S460_D464delSREYD

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3R1 (PIK3R1 Human)

Use

Lentiviral

Mutation

S460_D464delSREYD

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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Lenti-CRISPR-V2-gYap-2

Plasmid

#166482

Purpose

Expresses spCas9 and a gRNA targeting mouse Yap

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

gRNA for mouse Yap (Yap1 Mouse)

Use

Lentiviral

Available Since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CB-L1049R

Plasmid

#116557

Purpose

Lentiviral expression of PIK3CB L1049R

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CB (PIK3CB Human)

Use

Lentiviral

Mutation

L1049R

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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