We narrowed to 3,866 results for: mak

1. HP138-puro

   Plasmid

   #134246

   Purpose

   Cas9 in a transposon array under a Tet inducible promoter. For making inducible Cas9 cell lines. Puro selection.

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   Contains Cas9
   

   Use

   CRISPR

   Available Since

   Feb. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYPQ133-tRNA2.0

   Plasmid

   #158395

   Purpose

   Golden Gate entry vector to express the 3rd gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Promoter

   Without promoter

   Available Since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCk3_spacer

   Plasmid

   #136073

   Purpose

   Use when making even level constructs (L2,...) if pCk3 position (position 3) has no construct assigned

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   Odd spacer
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Available Since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. MF87_PB-EF1a-TET3G-P2A-ERT2-Gal4-BGHpA

   Plasmid

   #176630

   Purpose

   Integrated in CHO-K1 to make parental polyclonal lines for all other MultiFate cell lines

   Depositor

   Michael Elowitz

   Article

   Zhu et al Science. 2022 Jan 21;375(6578):eabg9765.

   Insert

   EF1a-TET3G-P2A-ERT2-Gal4-BGHpA
   

   Use

   Synthetic Biology

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYPQ134-tRNA2.0

   Plasmid

   #158396

   Purpose

   Golden Gate entry vector to express the 4th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Promoter

   Without promoter

   Available Since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. (R42A) Cyclin B1-GFP (62)

   Plasmid

   #61849

   Purpose

   mammalian expression of Cyclin B1 with R42A mutation in destruction box to make cyclin B1 non-destructable

   Depositor

   Jonathon Pines

   Article

   Clute et al Nat Cell Biol. 1999 Jun;1(2):82-7.

   Insert

   cyclin B1 (CCNB1 Human)
   

   Tags

   GFP and Myc

   Expression

   Mammalian

   Mutation

   V5A R42A

   Promoter

   CMV

   Available Since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYPQ135-tRNA2.0

   Plasmid

   #158397

   Purpose

   Golden Gate entry vector to express the 5th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Promoter

   Without promoter

   Available Since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAID 1.1 N-T2A-Bsr

   Plasmid

   #105985

   Purpose

   To make an auxin inducible degron mutant

   Depositor

   Tatsuo Fukagawa

   Article

   Nishimura et al Chromosome Res. 2017 Oct;25(3-4):253-260

   Insert

   T2A-Bsr
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYPQ136-tRNA2.0

   Plasmid

   #158398

   Purpose

   Golden Gate entry vector to express the 6th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Promoter

   Without promoter

   Available Since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCsC_spacer

    Plasmid

    #136077

    Purpose

    Use when making odd level constructs (L3,...) if pCsC position (position 3) has no construct assigned

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    Even spacer
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pCsE_spacer

    Plasmid

    #136078

    Purpose

    Use when making odd level constructs (L3,...) if pCsE position (position 4) has no construct assigned

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    Even spacer
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. modRNAc0-Cas9

    Plasmid

    #170180

    Purpose

    IVT template plasmid for making modified Cas9 RNA

    Depositor

    Xiaojun Lian

    Article

    Haideri et al Cell Rep Methods. 2022 Sep 7;2(9):100290

    Insert

    Cas9
    

    Use

    Ivt template plasmid

    Promoter

    T7 promoter

    Available Since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAID 1.1C-T2A-Bsr

    Plasmid

    #105986

    Purpose

    To make an auxin inducible degron mutant

    Depositor

    Tatsuo Fukagawa

    Article

    Nishimura et al Chromosome Res. 2017 Oct;25(3-4):253-260

    Insert

    T2A-Bsr
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDZ617 pKAN 12xPP7 V4

    Plasmid

    #72237

    Purpose

    Floxed Kanamycin (G418) selection maker; 3' tagging vector used to add 12 repetitive PP7 version 4 RNA stem-loops to gene of interest

    Depositor

    Daniel Zenklusen

    Article

    Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

    Insert

    12xPP7 V4
    

    Use

    Cre/Lox

    Expression

    Bacterial

    Available Since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCk1_spacer

    Plasmid

    #136071

    Purpose

    Use when making even level constructs (L2,...) if pCk1 position (position 1) has no construct assigned

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    Odd spacer
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pLV GG hUbV-EGFP

    Plasmid

    #216161

    Purpose

    Contains a Golden-Gate cloning cassette to express up to four gRNA and EGFP

    Depositor

    Makiko Iwafuchi

    Article

    Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. CaP2AIII_pK18

    Plasmid

    #153421

    Purpose

    Source of murine TCR-alpha constant region (TRAC*02) gene followed by P2A

    Depositor

    Maki Nakayama

    Article

    Anderson et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

    Insert

    murine TCR-alpha constant region (TRAC*02)-P2A
    

    Expression

    Bacterial

    Available Since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. HOXC13(human)-pET

    Plasmid

    #8679

    Depositor

    Corey Largman

    Article

    Komuves et al Dev Dyn 2003 Jun;227(2):192-202.

    Insert

    HOXC13 (HOXC13 Human)
    

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    makes protein by Coomassie blue staining

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

19. pNS3

    Plasmid

    #131770

    Purpose

    PADH1_FUSN_Fusion Red_Cry2Olig_TACT1 for Integration into δ-sites

    Depositor

    Jared Toettcher

    Article

    Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

    Insert

    PADH1_FUSN_Fusion Red_Cry2Olig_TACT1
    

    Use

    Integration into δ-sites

    Expression

    Yeast

    Mutation

    E490G to make Cry2 into Cry2Olig

    Promoter

    PADH1

    Available Since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pU6nH1-HD10kb

    Plasmid

    #172657

    Purpose

    Expressing paired pegRNAs from human U6 and H1 promoters to make 10204-bp deletion on HPRT1 gene

    Depositor

    Jay Shendure

    Article

    Choi et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

    Insert

    pegRNA-HD10kbA/pegRNA-HD10kbB
    

    Use

    CRISPR

    Available Since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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