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Plasmid#165078PurposeExpression of RfxCas13d sgRNAs from a U6 promoterDepositorInsertScaffold gRNA
ExpressionMammalianAvailable SinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
HA-SFPQ
Plasmid#166959PurposeExpresses HA-tagged SFPQ protein in pcDNA3.1DepositorAvailable SinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEG BacMam C term 3C eGFP His8
Plasmid#160687PurposeVector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target proteinDepositorTypeEmpty backboneTags3C eGFP His8ExpressionMammalianPromoterCMVAvailable SinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-mDlx-GFP-Fishell-1 (AAV Retrograde)
Viral Prep#83900-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-mDlx-GFP-Fishell-1 (#83900). In addition to the viral particles, you will also receive purified pAAV-mDlx-GFP-Fishell-1 plasmid DNA. mDlx-driven expression of GFP in GABA-ergic interneurons. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromotermDlxTagsGFPAvailable SinceJune 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetOminiCMV-OSK
Plasmid#136552PurposeDox-inducible polycistronic reprogramming lentiviral vector expressing mouse Oct4, Sox2, Klf4DepositorUseLentiviralExpressionBacterialPromotertetO-miniCMV (dox-inducible)Available SinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
BII-CMV-AfmW3A
Plasmid#133402PurposePiggybac vector for constitutive expression of Afamin + Wnt3a for production of conditioned media in HEK293T cellsDepositorAvailable SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV5)
Viral Prep#112677-AAV5PurposeReady-to-use AAV5 particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsmCherry (Cre-negative cells), EGFP (Cre-positive cells)Available SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-C-terminal GST
Plasmid#128025PurposeMammalian expression vector with C terminal GST tagDepositorTypeEmpty backboneTagsGSTExpressionMammalianAvailable SinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_MMP-9-HA
Plasmid#121172PurposeExpression of mouse MMP9DepositorInsertMMP9 (matrix metallopeptidase 9) (Mmp9 Mouse)
TagsHAExpressionMammalianMutationSee depositor comments belowPromoterCMV immediate/earlyAvailable SinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
HIV-1 LTR-gfp
Plasmid#115809PurposeHIV-1 LTR driven reporter vector that retains complete LTRs, tat, and rev, but has a frameshift mutation in env, an ngfr reporter gene in place of nef, and gfp in place of gag, pol, vif, and vprDepositorInsertgfp and ngfr
UseLentiviralPromoterHIV1 LTRAvailable SinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSK9065
Plasmid#84345PurposeTightly controlled expression of mRFP fusion proteins in S. aureusDepositorTypeEmpty backboneTagsmRFPmarsExpressionBacterialPromoterPxyl/tetAvailable SinceNov. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBiex-1 GFPNanobody_SNAP
Plasmid#82711PurposeExpresses a GFP binding nanobody linked to a SNAP tag in bacteria and Sf9 cells.DepositorInsertGFP Nanobody
Tags6xHis Purification tag, FLAG purification tag, an…ExpressionBacterial and InsectPromoterT7 PromoterAvailable SinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET22b_PstS_1
Plasmid#78198PurposeExpression of single-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensorDepositorInsertpstS (A197C)
ExpressionBacterialMutationA197C (please see depositors comment below)PromoterT7Available SinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Rab9a
Plasmid#78592PurposeExpress mCherry-Rab9a in mammalian cellsDepositorAvailable SinceJune 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-MTOR-L1460P
Plasmid#69006Purposeactivating MTOR mutationDepositorInsertMTOR-L1460P (MTOR Human)
TagsFLAGExpressionMammalianMutationchange Leucine 1460 to ProlineAvailable SinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCW-Flag-CtIP
Plasmid#71108Purposemammalian expession of Flag-CtlPDepositorInsertCtlP
UseLentiviralTagsFlagExpressionMammalianMutationPlease see Depositor CommentsPromoterTRE promoter, Tet ONAvailable SinceNov. 11, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pU6-(BbsI)_CBh-Cas9-T2A-mCherry-H1-(BamHI)
Plasmid#64217PurposeExpression vector for sgRNAs cloned into the BbsI sites, shRNAs into BamHI & AflII and for Expression of Cas9 linked to mCherry via T2ADepositorInsertsCas9
hU6 promoter; BbsI sites for sgRNA
H1 promoter; BamHI site for shRNA
UseCRISPRTags3xFLAG, NLS, and T2A-mCherryExpressionMammalianPromoterCBh, H1, and U6Available SinceSept. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UbC
Plasmid#62806PurposeTo express genes at high levels in neuronal cells. This UbC promoter is more active in neurons than the promoter in CMV-based vectors.DepositorTypeEmpty backboneUseAAV; EucaryoticExpressionMammalianPromoterhUbCAvailable SinceMarch 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
FUW-tetO-loxP-hKLF2
Plasmid#60850PurposeLentiviral DOX-inducible expression vector for human KLF2DepositorInsertKLF2 (KLF2 Human)
UseLentiviralAvailable SinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAG_mRuby2_smFP OLLAS
Plasmid#59761Purposeencodes multiple copies of an epitope tag OLLAS ( not detected by anti GFP ab)DepositorInsertmRuby2_smFP_OLLAS
ExpressionMammalianPromoterCAGAvailable SinceOct. 21, 2014AvailabilityAcademic Institutions and Nonprofits only