176,505 results

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Tet-inducible Luciferase reporter

Plasmid

#64127

Purpose

Photoactivatable transcription system. Luciferase reporter under the control of a tet-inducible promoter.

Depositor

Moritoshi Sato

Article

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

luciferase

Use

Luciferase

Expression

Mammalian

Promoter

TetOx13-CMV

Available Since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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pET28a-EGFP-CNA35

Plasmid

#61603

Purpose

bacterial expression of N-terminal fusion protein of CNA35 with EGFP

Depositor

Maarten Merkx

Article

Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

Insert

EGFP and CNA35

Tags

His tags

Expression

Bacterial

Promoter

T7

Available Since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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EGFP-Tubulin-6

Plasmid

#56450

Purpose

Localization: Microtubules, Excitation: 488, Emission: 507

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

Tubulin (TUBA1B Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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mEmerald-Mito-7

Plasmid

#54160

Purpose

Localization: Mitochondria, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Planchon et al Nat Methods. 2011 May;8(5):417-23. doi:

Insert

Mito (COX8A Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pet30b-5M SrtA

Plasmid

#51140

Purpose

expression plasmid for C-terminal his tagged S. aureus SrtA with enhanced catalytic activity

Depositor

Hidde Ploegh

Article

Shi et al Proc Natl Acad Sci U S A. 2014 Jul 15;11

Insert

S.aureus SrtA 5M

Tags

6x His

Expression

Bacterial

Mutation

P94R, D160N, D165A, K190E, K196T

Promoter

T7

Available Since

Feb. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pPAmCherry-b-actin

Plasmid

#31949

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

Insert

PAmCherry1

Tags

b-actin

Expression

Mammalian

Mutation

E26V/A58T/K69N/L84F/N99K/S148L/I165V/Q167P/L169V/…

Promoter

CMV

Available Since

Sept. 22, 2011

Availability

Academic Institutions and Nonprofits only

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pLSSmKate2-N1

Plasmid

#31867

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

LSS-mKate2

Expression

Mammalian

Mutation

K69Y/P131T/S148G/M167D/T183S/M196V

Promoter

CMV

Available Since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

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pPAmCherry1-C1

Plasmid

#31929

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

Insert

PAmCherry1

Expression

Mammalian

Mutation

E26V/A58T/K69N/L84F/N99K/S148L/I165V/Q167P/L169V/…

Promoter

CMV

Available Since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only

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pWZL-Blast-Flag-HA-hTERT

Plasmid

#22396

Depositor

William Hahn

Article

Maida et al Nature. 2009 Sep 10. 461(7261):230-5.

Insert

TERT (TERT Human)

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Available Since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/opto-b2AR-EYFP

Plasmid

#20948

Purpose

Mammalian expression of opto-b2AR fused to EYFP for optical control of GPCR signaling

Depositor

Karl Deisseroth

Article

Airan et al Nature. 2009 Apr 23;458(7241):1025-9. do

Insert

opto-b2AR

Expression

Mammalian

Available Since

May 28, 2009

Availability

Academic Institutions and Nonprofits only

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pMSP1E1

Plasmid

#20062

Depositor

Stephen Sligar

Article

Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

Insert

MSP1E1 (APOA1 synthetic gene)

Tags

6-His

Expression

Bacterial

Mutation

"extended" MSP1; contains repeat of hel…

Available Since

Jan. 12, 2009

Availability

Academic Institutions and Nonprofits only

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shCD44-2 pRRL

Plasmid

#19123

Depositor

Bob Weinberg

Article

Godar et al Cell. 2008 Jul 11. 134(1):62-73.

Insert

small hairpin RNA against CD44 (CD44 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Oct. 2, 2008

Availability

Academic Institutions and Nonprofits only

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pET11phiC31poly(A)

Plasmid

#18942

Depositor

Michele Calos

Article

Groth et al Genetics. 2004 Apr . 166(4):1775-82.

Insert

phiC31 integrase (int Streptomyces phage phiC31)

Expression

Bacterial

Available Since

Aug. 13, 2008

Availability

Academic Institutions and Nonprofits only

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LAMP1-GFP

Plasmid

#16290

Depositor

Ron Vale

Article

Minin et al J Cell Sci. 2006 Feb 15. 119(Pt 4):659-7

Insert

LAMP1/RE72002

Tags

EGFP

Expression

Insect

Promoter

Ac5

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

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PIK3CA - Exon20 H1047R

Plasmid

#16639

Depositor

Bert Vogelstein

Article

Samuels et al Science. 2004 Apr 23. 304(5670):554.

Insert

PIK3CA H1047R (PIK3CA Human)

Tags

Flag

Expression

Mammalian

Mutation

H1047R

Available Since

March 10, 2008

Availability

Academic Institutions and Nonprofits only

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pLenti-pHluorin_M153R-CD63

Plasmid

#172117

Purpose

Fluorescent reporter for CD63-positive exosome secretion

Depositor

Alissa Weaver

Article

Sung et al Nat Commun. 2020 Apr 29;11(1):2092. doi:

Insert

CD63 (CD63 Human)

Use

Lentiviral

Tags

pHluorin_M153R in a small extracelluar loop of CD…

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1(nsp8-nsp7)(nsp12)

Plasmid

#165451

Purpose

Expresses the RNA Dependent RNA polymerase complex of SARS-CoV-2 in Escherichia Coli

Depositor

Marc Delarue

Article

Madru et al PLoS One. 2021 Apr 29;16(4):e0250610. do

Inserts

nsp12
nsp8
nsp7

Tags

14Histidine-TEV

Expression

Bacterial

Promoter

T7

Available Since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-syn-jGCaMP8f-WPRE

Plasmid

#162376

Purpose

AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Has Service

AAV Retrograde, AAV1, AAV5, and AAV9

Insert

jGCaMP8f

Use

AAV

Tags

6xHis

Expression

Mammalian

Mutation

Q315L

Promoter

Synapsin

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2+IMS-HyPer7

Plasmid

#136469

Purpose

Mammalian expression of mitochondrial intermembrane space targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

Depositor

Vsevolod Belousov

Article

Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

Insert

HyPer7

Tags

SMAC/DIABLO

Expression

Mammalian

Promoter

CMV, SP6

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-EGFP (AAV2)

Viral Prep

#50457-AAV2

Purpose

Ready-to-use AAV2 particles produced from pAAV-hSyn-DIO-EGFP (#50457). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-EGFP plasmid DNA. hSyn-driven, Cre-dependent EGFP expression. These AAV preparations are suitable purity for injection into animals.

Depositor

Bryan Roth

Promoter

Syn

Tags

EGFP (Cre-dependent)

Available Since

Jan. 17, 2020

Availability

Academic Institutions and Nonprofits only