269,421 results
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Plasmid#70282PurposeColK-based vector; provides a nicking function to permit conjugal transfer and bears a polylinker for insertion of methylase-encoding genesDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only
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pSPORT1[NheI/glGFP/bglobin3'-UTR/Ins2]
Plasmid#74101Purposevector bearing an NheI restriction site for the two-step cloning method in Wang and Szaro, Dev Biol, 2015DepositorInsertsGreen Lantern Green Fluorescent Protein
Rabbit beta 1-globin gene 3'UTR
UseTagsExpressionMutationPromoterAvailable sinceMay 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA RBD-GFP11
Plasmid#133069PurposeExpresses RBD domain with tripartite split GFP11 (M4)DepositorInsertRBD (Rhotekin-Rho Binding Domain)
UseTagsGFP11 (tripartite split-GFP)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
YFP Gamma 3
Plasmid#36103DepositorInsertG protein subunit Gamma 3 (Gng3 Mouse)
UseTagsYFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDS-Red-N1 GABAA beta3
Plasmid#109143PurposeThe expression vector carries the red fluorescent protein tagged at the C-terminus of beta3 subunit of the GABAa receptor.DepositorInsertGABAa receptor
UseTagsRFPExpressionMammalianMutationbeta3 subunit of the GABAa receptorPromoterCMVAvailable sinceJune 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Ec.coaBC (pCLK1210)
Plasmid#50448PurposeHeterologous expression of E. coli CoA biosynthetic enzyme, PPCS_PPCDC - phosphopantothenoylcysteine synthetase_phosphopantothenoylcysteine decarboxylaseDepositorInsertPPCS PPCDC
UseTags6XHIS tagExpressionBacterialMutationPromoterT7Available sinceMarch 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRVV661
Plasmid#108282PurposeDonor vector for creation of PhiC31 attP-flanked platforms for cassette exchange using CRISPR/Cas9DepositorInsertPhiC31 attP sites
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEHIShqprt
Plasmid#92166PurposePlasmid pEHIShqprt expressing the full-length of human quinolinate phosphoribosyltransferase (hQPRTase) was constrcucted by inserting BspHI/BamHI-fragment into NcoI/BamHI digested pEHISTEV vectorDepositorInsertQuinolinate Phosphoribosyltransferase (QPRT Human)
UseTagsTEV protease -cleavable N-terminal 6HisExpressionBacterialMutationchanged Asp 2 to Asn to create BspHI sitePromoterT7Available sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRAM18dSGK[MCS]
Plasmid#84684PurposeCan be used for the transformation of rickettsiaDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTRANS_232
Plasmid#91121PurposeTransformation, Type: T-DNA, Plant Selection: 2x35S:bar, Viral Replication: ToLCVDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoter35SAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBABE.puro/TO/Flag/tGFP-HuR.wt
Plasmid#110349PurposeDoxycycline inducible mammalian retroviral expression of HuR (wild type) with FLAG and turboGFP tagsDepositorInsertELAV like RNA binding protein 1 (ELAVL1 Human)
UseRetroviralTagsFLAG and tGFPExpressionMammalianMutationPromotergagAvailable sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
dTomato_LD 0_CS M_tTA-BFP
Plasmid#58875PurposeMESA target chain with dTomato ectodomain, no linker domain, M cleavage sequence, and tTA-BFP fusionDepositorInsertMESA target chain with dTomato ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (M in P1')
UseAAVTagsEBFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
TFORF1868
Plasmid#141878PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertMMP3 (MMP3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pBJ-mPar3
Plasmid#53230Purposemammalian expression of mouse Par3DepositorInsertPar3 (F2rl2 Mouse)
UseTagsFLAG and bovine prolactin signal sequence (MDSKGS…ExpressionMammalianMutationDeletion of aa1-15 (native signal peptide)PromoterAvailable sinceAug. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
891 pFastBac GST PTEN
Plasmid#10937DepositorInsertPTEN (PTEN Human)
UseTagsGSTExpressionInsectMutationPromoterAvailable sinceJan. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET21 Catalytic Domain of MALT1
Plasmid#48970PurposeExpresses catalytic domain of MALT1 in e.coli AA 329-566DepositorInsertMALT1 (MALT1 Human)
UseTagsHisExpressionBacterialMutationContains AA 329-566PromoterT7Available sinceOct. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCfB2195
Plasmid#67548PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked hphMX marker, integration into S. cerevisiae XI-3 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeTagsExpressionYeastMutationPromoterAvailable sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
p3 FLAG_CMV14/B0,+
Plasmid#112798PurposeExpression of ATB0,+DepositorInsertB(0,+)/SLC6A14 (Slc6a14 Rat)
UseTags3 x FlagExpressionMammalianMutationL350SPromoterAvailable sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQStrep2-PREI3
Plasmid#31592DepositorInsertpreimplantation protein 3 (MOB4 Human)
UseTagsHis and StrepII-tagExpressionBacterialMutationPromoterAvailable sinceApril 9, 2013AvailabilityAcademic Institutions and Nonprofits only