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pCMX/cyclinB1 (17)

Plasmid

#26060

Depositor

Jonathon Pines

Article

Hagting et al Curr Biol. 1999 Jul 1. 9(13):680-9.

Insert

Cyclin B1 (CCNB1 Human)

Tags

Myc

Expression

Mammalian

Available Since

Aug. 27, 2010

Availability

Academic Institutions and Nonprofits only
pX330A_dCas9-1x3

Plasmid

#63597

Purpose

Expresses dCas9 and gRNA

Depositor

Takashi Yamamoto

Article

Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

Insert

humanized S. pyogenes dCas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

June 1, 2015

Availability

Academic Institutions and Nonprofits only

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pMaCTag-05

Plasmid

#119984

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with eGFP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

eGFP

Use

Pcr template

Tags

eGFP

Promoter

None

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pMC-9-PinII-11

Plasmid

#197769

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

PinII terminator

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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GST HP1 gamma

Plasmid

#24076

Depositor

Naoko Tanese

Article

Vassallo et al Proc Natl Acad Sci U S A. 2002 Apr 30. 9

Insert

HP1 gamma (CBX3 Human)

Tags

GST

Expression

Bacterial

Available Since

Oct. 27, 2010

Availability

Academic Institutions and Nonprofits only
GST HP1 beta

Plasmid

#24075

Depositor

Naoko Tanese

Article

Vassallo et al Proc Natl Acad Sci U S A. 2002 Apr 30. 9

Insert

HP1 beta (CBX1 Human)

Tags

GST

Expression

Bacterial

Available Since

Oct. 27, 2010

Availability

Academic Institutions and Nonprofits only
pX330A-1x3

Plasmid

#58767

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Orange_CaMBI_110

Plasmid

#124094

Purpose

orange calcium-modulated bioluminescent indicator, 110nM

Depositor

Michael Lin

Article

Oh et al Nat Chem Biol. 2019 Apr 1. pii: 10.1038/

Insert

Orange CaMBI 110

Expression

Mammalian

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pX330S-7

Plasmid

#58783

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-luxOFP

Plasmid

#229950

Purpose

Mammalian expression of a de novo designed, FRET-based neoLux1.2-CyOFP1 fusion bioluminescent probe (max. Em=588)

Depositor

Andy Yeh

Article

Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

Insert

luxOFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/Puro/TagRFP-LL5b

Plasmid

#112828

Purpose

transient/stable overexpression

Depositor

Tomasz Prószyński, Joshua Sanes

Article

Proszynski et al J Cell Sci. 2013 May 15;126(Pt 10):2225-

Insert

LL5b (Phldb2 Mouse)

Tags

TagRFP

Expression

Mammalian

Available Since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pMC-6-StayGold-9

Plasmid

#197734

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

StayGold GFP

Expression

Plant

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-iRFP

Plasmid

#64887

Purpose

Marking retinal neurons with infrared fluorescent proteins (iRFP) to preserve photoresponsiveness

Depositor

Tomomi Ichinose

Article

Fyk-Kolodziej et al Biotechniques. 2014 Nov 1;57(5):245-53.

Insert

iRFP

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pX330S-5

Plasmid

#58781

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pX330A-1x6

Plasmid

#58770

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pCAG-OSF-PP

Plasmid

#154247

Purpose

backbone with PreScission protease-cleavable N-terminal OneStrepFLAG tag

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Type

Empty backbone

Tags

FLAG and Strep-Tag II

Expression

Mammalian

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR221-mCherry

Plasmid

#79509

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) mCherry RFP CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

mCherry RFP

Use

Gateway Cloning

Promoter

no Promoter

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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pBS-TarHom-Tol2-kanaR-cryaa-CFP

Plasmid

#74151

Purpose

BAC targeting cassette for pTARBAC2 with kanaR selection marker and lens-specific transgenesis marker Cerulean

Depositor

Holger Knaut

Article

Fuentes et al G3 (Bethesda). 2016 Jan 27. pii: g3.115.

Insert

Tol2-kanaR-cryaa-CFP

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-P-RAN-H2A2B

Plasmid

#106412

Purpose

P-RAN-H2A2B (RANbody H2A2B::vHRP)

Depositor

Joshua Sanes

Article

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Insert

P-RAN-H2A2B

Tags

HA and His

Expression

Mammalian

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pMC-1-AtUbi10-6

Plasmid

#197700

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

AtUbi10 promoter

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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