We narrowed to 10,362 results for: Coli
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Plasmid#79054PurposeExpresses SpyTagged GlpG proteinDepositorInsertSpyTag-GlpG (glpG )
Tags6xHis and SpyTagExpressionBacterialMutationGlpG protein employed here corresponds to residue…PromoterTrcAvailable SinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
BPK2014-AmCas12b
Plasmid#121951PurposeE. coli expression, protein purificationDepositorInsertAmCas12b
Tags10xHis tagExpressionBacterialAvailable SinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRL1124
Plasmid#70690Purposeprovides avaiM, eco47iiM, and ecot22iM that encode specific methylases to enable transfer of plasmids from E. coli to Anabaena sp. strain PCC 7120 and other strains. lacks a mobilization function.DepositorInsertsavaiM
eco47iiM
ecot22iM
UseBacterial shuttle vectorAvailable SinceNov. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pQE30-AtAEP2
Plasmid#159529PurposeExpresses Arabidopsis thaliana asparaginyl endopeptidase 2 in E. coliDepositorInsertArabidopsis thaliana asparaginyl endopeptidase 2
TagsSix-Histidine tagExpressionBacterialPromoterT5Available SinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQE30-CeAEP1
Plasmid#159528PurposeExpresses jack bean (Canavalia ensiformis) asparaginyl endopeptidase 1 in E. coliDepositorInsertCanavalia ensiformis asparaginyl endopeptidase 1
TagsSix-Histidine tagExpressionBacterialPromoterT5Available SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMH-HT_SsCHAD
Plasmid#127783PurposeExpresses SsCHAD in E. coli cellsDepositorInsertSsCHAD (SULM_RS11045 Sulfolobus solfataricus)
Tags6xHis tag + TEV cleavage siteExpressionBacterialAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-HsSENP7
Plasmid#71470PurposeEncodes human full length SENP7. Suitable for in vitro transcription/translation (T7).DepositorInsertSUMO1/sentrin specific peptidase 7 (SENP7 Human)
TagsHexahistidineExpressionBacterialMutationGene optimised for E. coli expression (Genscript)PromoterT7Available SinceJan. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-human Beta-catenin R1C (138–781)
Plasmid#132608Purposeexpresses human beta-catenin R1C (138–781) in E. coliDepositorInsertBeta-catenin R1C (138–781)
ExpressionBacterialAvailable SinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-3xFLAG-MyD88-GyrB
Plasmid#131350PurposeExpresses MyD88 with an N-termial 3xFLAG tag and a C-terminal GyrB domain from the E. coli DNA Gyrase BDepositorAvailable SinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUDP003
Plasmid#101164PurposeE. coli/S. cerevisiae amdS shuttle vector allowing cloning of ribozyme flanked g-RNA for Cas9 editing (HH-gRNA-HDV)DepositorTypeEmpty backboneUseCRISPRExpressionYeastPromoterScTDH3Available SinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET_His6-MBP-TEV-Cas2
Plasmid#186275PurposeExpresses Thiomicrospira Cas2 in E. coliDepositorInsertThiomicropira Cas2
TagsHis6-MBPExpressionBacterialAvailable SinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21b - S. thermophilus DltB
Plasmid#132607Purposeexpresses S. thermophilus DltB in E. coliDepositorInsertDltb
ExpressionBacterialAvailable SinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
hisSUMO-mZFP57
Plasmid#87224PurposeExpress hisSUMO tagged Zinc finger protein 57 F1-F3(mouse) in E. coliDepositorAvailable SinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQE30-HaAEP1
Plasmid#159530PurposeExpresses sunflower (Helianthus annuus) asparaginyl endopeptidase 1 in E. coliDepositorInsertHelianthus annuus asparaginyl endopeptidase 1 (LOC110922574 Synthetic)
TagsSix-Histidine tagExpressionBacterialPromoterT5Available SinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRL2697
Plasmid#70693PurposepDU1-E. coli shuttle vector (Cm-r Em-r) with extended polylinkerDepositorTypeEmpty backboneUseBacterial shuttle vectorAvailable SinceNov. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-32M human DKK1c (183–266)
Plasmid#132626Purposeexpresses human DKK1c (183–266) in E. coliDepositorInsertDKK1 (183–266)
ExpressionBacterialAvailable SinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28b-trAtCCD8(pYL736)
Plasmid#178287PurposeExpresses trAtCCD8 in E. coli. pET28b carrying t N-terminus 56 amino-acid truncated CCD8 from ArabidopsisDepositorInserttrAtCCD8
ExpressionBacterialPromoterT7Available SinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSB1A2QS
Plasmid#138268PurposeMINPA Gene circuit expression in E. coli. GFP serves as a reporter for Plux/tet promoter activity. mCherry serves as reporter for Para/lac promoter activityDepositorInsertsAraC-TetR operon
LuxR-LacI operon
mCherry
GFP
UseSynthetic BiologyExpressionBacterialPromoterPara/lac and Plux/tetAvailable SinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJZ002
Plasmid#120231PurposeCRISPR-cas9 targeting in E. coli with ampicillin resistanceDepositorTypeEmpty backboneUseCRISPRAvailable SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-SAR1
Plasmid#67388PurposeExpression of native hSar1 in E. coliDepositorAvailable SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTYF-2xGfaABC1D-tTA
Plasmid#19981DepositorInsert2xGfaABC1D-tTA
ExpressionMammalianAvailable SinceAug. 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
pTH1-SaSrtA-KA
Plasmid#64981PurposeExpresses the E105K/E108A mutant of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
TagsHis6 tag, Mxe GyrA intein, and chitin-binding pro…ExpressionBacterialMutationdeleted amino acids 1-59, E105K, E108AAvailable SinceJune 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPD3C
Plasmid#86971PurposeExpresses PCNA3 protein fused phosphite dehydrogenase and P450cam in E. coliDepositorInsertPCNA3 fused to phosphite dehydrogenase and P450cam
TagsHis6 tagExpressionBacterialMutationE175A/A176R in PTDH, N106R in PCNA3, silent mutat…PromoterT7 promoterAvailable SinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGHNC5
Plasmid#24701DepositorInsertDirect Repeats of Tobacco Rb7-6 MARs
UseE. coli phagemidAvailable SinceJuly 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
pfMDH_H303A
Plasmid#67730PurposeExpresses H303A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coliDepositorInsertMannitol 2-Dehydrogenase from Pseudomonas fluorescens
Tags6x-His tagExpressionBacterialMutationChanged histidine 303 to alaninePromoterT7Available SinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pfMDH_D230A
Plasmid#67725PurposeExpresses D230A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coliDepositorInsertMannitol 2-Dehydrogenase from Pseudomonas fluorescens
Tags6x-His tagExpressionBacterialMutationChanged aspartate 230 to alaninePromoterT7Available SinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pfMDH_N191A
Plasmid#67724PurposeExpresses N191A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coliDepositorInsertMannitol 2-Dehydrogenase from Pseudomonas fluorescens
Tags6x-His tagExpressionBacterialMutationChanged asparagine 191 to alaninePromoterT7Available SinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pfMDH_K295A
Plasmid#67726PurposeExpresses K295A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coliDepositorInsertMannitol 2-Dehydrogenase from Pseudomonas fluorescens
Tags6x-His tagExpressionBacterialMutationChanged lysine 295 to alaninePromoterT7Available SinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEXndoE(E662Q)
Plasmid#47716PurposeExpresses GST-EndoE(E662Q) from E. faecalis in E. coliDepositorInsertndoE
TagsGST-EndoE(E186Q) and Glutathione S-transferaseExpressionBacterialMutationChanged Glu-662 to GlnPromotertacAvailable SinceJan. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC19-P70a-Top-T~Bottom-T
Plasmid#87315PurposeControl plasmid. Encodes the Split-Broccoli system (Top + Bottom) with only "Top" under the control of E. coli sigma 70 promoter.DepositorInsertSplit-Broccoli system (promoter control)
UseSynthetic BiologyExpressionBacterialPromoterP70aAvailable SinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1 human APC-2,3 (1362–1540)
Plasmid#132631Purposeexpresses human APC-2,3 (1362–1540) in E. coliDepositorInsertAPC-2,3 (1362–1540)
ExpressionBacterialAvailable SinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSB4C5_J23101-CocE
Plasmid#128129PurposeExpressing CocE enzyme (cocaine to benzoate) gene under control of the constitutive promoter J23101, and RBS B0032DepositorInsertCocE
UseSynthetic BiologyExpressionBacterialPromoterJ23101Available SinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRBBm76
Plasmid#48117PurposeShuttle vector E. coli/B.meg.DepositorInsertpromoter sacB - gfp
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCDB326
Plasmid#113670Purposeexpresses Ulp1_R2 in Escherichia coliDepositorInsertUlp1_R2
Tags6-HisExpressionBacterialMutationcontains solubility enhancing mutationsPromoterT7Available SinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-21a-GFP-Patronin CC 1288–1457
Plasmid#59055Purposefor E. coli expression of Patronin CC truncationDepositorAvailable SinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1-GST-CD-ATLm(Cbx7)
Plasmid#82528PurposeExpresses mutant GST-CD-ATLm (Cbx7) fusion proteins in E. coli. Cbx7 amino acids 1-84 with mutation of RKR70-72, K75, and KR77-78 to AGA70-72, A75, and AG77-78.DepositorInsertChromobox Homolog 7 (CBX7 Human)
TagsGSTExpressionBacterialMutationamino acids 1-84 with mutation of RKR70-72, K75, …Available SinceDec. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHL 1916
Plasmid#53023PurposeAmpR + PLtetO-1::RBS (st7) fimB::Asp terminator + ColE1DepositorInsertfimB
UseSynthetic BiologyTagsAsp terminator and RBS(st7)ExpressionBacterialPromoterpLtetO-1Available SinceMay 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 1011
Plasmid#52993PurposepLlac0-1:RhyB-mCherry, pLtetO-1:RBS (st7) gfpDepositorInsertsRhyB sRNA
RBS (st7)
UseSynthetic BiologyTagsGFP and RBS-mCherryExpressionBacterialPromoterpLlac0-1 and pLtetO-1Available SinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL1756
Plasmid#53036PurposePconNoHindM12:glgC::gfp, PconNoHind:RBS(st3):tetRDepositorInsertsGFP
tetR
UseSynthetic BiologyTagsRBS(st3) and glgC leaderExpressionBacterialPromoterpConNoHind and pConNoHindM12Available SinceMay 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRB7-6
Plasmid#24700DepositorInsertTobacco Rb7 MAR (SAR)
UseE. coli phagemidAvailable SinceJuly 8, 2010AvailabilityAcademic Institutions and Nonprofits only