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pZ P (cHS4)X4 TetON-3XFLAG-tdT CAGG-m2rtTA v2

Plasmid

#112669

Purpose

Donor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows inducible expression and contains cHS4 insulators.

Depositor

Catherine Verfaillie

Article

Ordovas et al Stem Cell Reports. 2015 Nov 10;5(5):918-

Insert

tdT

Use

AAV; Donor plasmid for recombinase-mediated casse…

Expression

Mammalian

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pCAGGS SARS-CoV-2 Delta Spike

Plasmid

#185593

Purpose

Expresses codon-optimized full length SARS-CoV-2 Delta Spike

Depositor

Marceline Côté

Article

Benlarbi et al iScience. 2022 Nov 18;25(11):105316. doi

Insert

SARS-CoV-2 Delta Spike (S )

Expression

Mammalian

Mutation

Contains the following mutations: T19R, Δ156-157,…

Promoter

chicken β-actin promoter, CMV enhancer

Available Since

June 8, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pEB1-mGFPmut2

Plasmid

#103980

Purpose

Plasmid encoding mGFPmut2

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mGFPmut2

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (S65A, V68L, …

Promoter

proC

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pCIRCNpu

Plasmid

#74226

Purpose

Vector for SICLOPPS-mediated protein circularization using the Nostoc punctiforme DnaE intein.

Depositor

Barbara Di Ventura, Roland Eils

Article

Waldhauer et al Mol Biosyst. 2015 Nov 10;11(12):3231-43.

Type

Empty backbone

Use

Synthetic Biology

Tags

Npu DnaE C-intein and Npu DnaE N-intein

Expression

Bacterial

Promoter

T7-lac

Available Since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG-FLEX.SF-iGluSnFR.A184S.mRuby3

Plasmid

#106204

Purpose

Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.A184S

Use

AAV

Mutation

GltI: A184S + mRuby3 fusion

Promoter

CAG

Available Since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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FlagETR3 (CELF2)

Plasmid

#96900

Purpose

expression of Flag-tagged human CELF2 (aka CUGBP2)

Depositor

Thomas Cooper

Article

Orengo et al Nucleic Acids Res. 2006;34(22):e148. Epu

Insert

human CELF2 cDNA (CELF2 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-uvsX-H6

Plasmid

#163913

Purpose

Expresses uvsX for bacterial expression and affinity purification

Depositor

Paul Freemont

Article

Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

Insert

uvsX

Tags

His

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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pEJS1096 Dual-sgRNA.Design 1

Plasmid

#159538

Purpose

Delivery of dual sgRNA cassettes and Nme2Cas9 in a single AAV vector

Depositor

Erik Sontheimer

Article

Ibraheim et al Nat Commun. 2021 Nov 1;12(1):6267. doi:

Insert

Nme2Cas9 nuclease with two guide RNA cassettes with promoters

Use

AAV, CRISPR, and Mouse Targeting

Tags

NLS

Expression

Mammalian

Promoter

U1a

Available Since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pCAGGS-SARS2-S-G614 (C-flag)

Plasmid

#156421

Purpose

Mammalian expression of SARS-CoV-2 S protein (G614) with the flag tag at C-terminus. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.

Depositor

Hyeryun Choe, Michael Farzan

Article

Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

Insert

SARS-CoV-2 S protein (S SARS CoV2)

Tags

Flag

Expression

Mammalian

Mutation

D614G

Promoter

CMV

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pEB1-mGFPmut2

Plasmid

#103980

Purpose

Plasmid encoding mGFPmut2

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mGFPmut2

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (S65A, V68L, …

Promoter

proC

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pCMMP-dnPERK-IRES-eGFP

Plasmid

#36954

Depositor

Bill Sugden

Article

Lee et al Blood. 2008 Feb 15;111(4):2280-9. Epub 2

Insert

dnPERK (EIF2AK3 Human)

Use

Retroviral

Expression

Mammalian

Mutation

deletion of aa592-1061 to create dominant negative

Promoter

CMV

Available Since

July 12, 2012

Availability

Academic Institutions and Nonprofits only

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pCIRCNpu

Plasmid

#74226

Purpose

Vector for SICLOPPS-mediated protein circularization using the Nostoc punctiforme DnaE intein.

Depositor

Barbara Di Ventura, Roland Eils

Article

Waldhauer et al Mol Biosyst. 2015 Nov 10;11(12):3231-43.

Type

Empty backbone

Use

Synthetic Biology

Tags

Npu DnaE C-intein and Npu DnaE N-intein

Expression

Bacterial

Promoter

T7-lac

Available Since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

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pET-Ran(Q69L)

Plasmid

#42048

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

Ran (RAN Human)

Tags

His

Expression

Bacterial

Mutation

Q69L

Available Since

Feb. 25, 2013

Availability

Academic Institutions and Nonprofits only

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FlagETR3 (CELF2)

Plasmid

#96900

Purpose

expression of Flag-tagged human CELF2 (aka CUGBP2)

Depositor

Thomas Cooper

Article

Orengo et al Nucleic Acids Res. 2006;34(22):e148. Epu

Insert

human CELF2 cDNA (CELF2 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pCMMP-dnPERK-IRES-eGFP

Plasmid

#36954

Depositor

Bill Sugden

Article

Lee et al Blood. 2008 Feb 15;111(4):2280-9. Epub 2

Insert

dnPERK (EIF2AK3 Human)

Use

Retroviral

Expression

Mammalian

Mutation

deletion of aa592-1061 to create dominant negative

Promoter

CMV

Available Since

July 12, 2012

Availability

Academic Institutions and Nonprofits only

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Human SLC SAM Library

Pooled Library

#132561

Purpose

Activation library targeting human SLC proteins.

Depositor

Giulio Superti-Furga

Article

Rebsamen et al Life Sci Alliance. 2022 Sep 16;5(11):e20

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available Since

May 18, 2020

Availability

Academic Institutions and Nonprofits only
pCMMP-MCS-IRES-Puro

Plasmid

#36952

Depositor

Bill Sugden

Article

Lee et al Blood. 2008 Feb 15;111(4):2280-9. Epub 2

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available Since

May 16, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pEJS1096 Dual-sgRNA.Design 1

Plasmid

#159538

Purpose

Delivery of dual sgRNA cassettes and Nme2Cas9 in a single AAV vector

Depositor

Erik Sontheimer

Article

Ibraheim et al Nat Commun. 2021 Nov 1;12(1):6267. doi:

Insert

Nme2Cas9 nuclease with two guide RNA cassettes with promoters

Use

AAV, CRISPR, and Mouse Targeting

Tags

NLS

Expression

Mammalian

Promoter

U1a

Available Since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pCAGGS-SARS2-S-G614 (C-flag)

Plasmid

#156421

Purpose

Mammalian expression of SARS-CoV-2 S protein (G614) with the flag tag at C-terminus. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.

Depositor

Hyeryun Choe, Michael Farzan

Article

Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

Insert

SARS-CoV-2 S protein (S SARS CoV2)

Tags

Flag

Expression

Mammalian

Mutation

D614G

Promoter

CMV

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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FDP-P10B2-A75-RZ-URA3

Plasmid

#130874

Purpose

Integration cassette for OrthoRep containing mKate driven by promoter 10B2 with a hardcoded polyA tail

Depositor

Chang Liu

Article

Zhong et al ACS Synth Biol. 2018 Dec 21;7(12):2930-2

Insert

mKate

Use

Expression of mkate, leu2(q180*), and ura3 on ort…

Available Since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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