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pHR-EF1a-dCas9-HA-BFP-KRAB-NLS

Plasmid

#102244

Purpose

Lentiviral expression of dCas9-HA-BFP-KRAB-NLS for CRISPRi driven by a hEF1alpha promoter

Depositor

Jacob Corn

Insert

dCas9-HA-BFP-KRAB-NLS

Use

CRISPR and Lentiviral

Tags

HA-TagBFP-KRAB-NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pPHT-PKA

Plasmid

#60936

Purpose

Fluorescent FPX sensor for cAMP

Depositor

Anne Marie Quinn

Article

Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

Insert

PKA FPX Sensor

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-CoChR-GFP

Plasmid

#59070

Purpose

AAV-mediated expression of CoChR-GFP under the Syn promoter

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

CoChR-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

Syn

Available Since

Aug. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pfwB

Plasmid

#37329

Depositor

John Schiller

Article

Buck et al J Virol. 2005 Mar;79(5):2839-46.

Insert

GFP

Expression

Mammalian

Promoter

EF1a

Available Since

July 20, 2012

Availability

Academic Institutions and Nonprofits only

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pHoss1

Plasmid

#63158

Purpose

Suicide plasmid for efficient gene mutation in Gram-positive bacteria

Depositor

Attila Karsi

Article

Abdelhamed et al Plasmid. 2015 May 31;81:1-8. doi: 10.101

Insert

anti-secY-Pxyl/tetO-tetR

Use

Suicide plasmid for gram-positive bacteria

Available Since

Sept. 29, 2015

Availability

Academic Institutions and Nonprofits only

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spMSP1D1

Plasmid

#173482

Purpose

Expresses spMSP1D1 in Bl21

Depositor

Huan Bao

Article

Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

Insert

spMSP1D1

Tags

Histag

Expression

Bacterial

Available Since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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dRT-pMDLg/pRRE

Plasmid

#60488

Purpose

gag/pol packaging plasmid for production of lentiviral vectors with mutated reverse transcriptase (NRTLV, non-reverse transcribable lentiviral vectors)

Depositor

Boris Fehse

Article

Mock et al Sci Rep. 2014 Sep 18;4:6409. doi: 10.103

Insert

HIV-1 gag, HIV-1 pol with inactive reverse transcriptase

Mutation

changed Asp at position 249 + 250 to Val

Available Since

Oct. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pPSU250

Plasmid

#173837

Purpose

Expresses 250 kD Penn State ladder protein

Depositor

Song Tan

Article

Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

Insert

STRHSTPABPACIL1bQRSSTRHSTPABMBPpepN

Expression

Bacterial

Promoter

T7

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pmiRFP720-N1

Plasmid

#136560

Purpose

A cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein miRFP720

Depositor

Vladislav Verkhusha

Article

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Insert

miRFP720

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCM1.53

Plasmid

#17250

Depositor

Geraldine Seydoux

Article

Merritt et al WormBook. 2010 Feb 8:1-21. doi: 10.1895/

Insert

GFP-no stop in pDONR201

Use

Gateway Cloning

Available Since

Feb. 26, 2008

Availability

Academic Institutions and Nonprofits only

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pDR-GW AT1.03

Plasmid

#28003

Depositor

Wolf Frommer

Article

Bermejo et al Biochem J. 2010 Dec 1. 432(2):399-406.

Insert

AT1.03

Tags

mVenus and mseCFP

Expression

Yeast

Available Since

Sept. 8, 2011

Availability

Academic Institutions and Nonprofits only

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pEGFP PICH (Nigg CB62)

Plasmid

#41163

Depositor

Erich Nigg

Article

Baumann et al Cell. 2007 Jan 12;128(1):101-14.

Insert

PICH (ERCC6L Human)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 7, 2013

Availability

Academic Institutions and Nonprofits only

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pCE35

Plasmid

#174409

Purpose

C. auris LEUpOUT NAT marker CAS9 expression construct. Use with pCE27 gRNA expression construct.

Depositor

Clarissa Nobile

Article

Ennis et al Microbiol Spectr. 2021 Dec 22;9(3):e0182

Insert

C. auris LEU2 1 of 2, pENO1, Cas9, NAT 1 of 2

Use

CRISPR

Expression

Bacterial

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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hGFAP-fLuc

Plasmid

#40589

Depositor

Albee Messing

Article

Cho et al J Neurochem. 2009 Jul;110(1):343-51. Epu

Insert

hGFAP promoter (GFAP Human)

Use

Luciferase

Tags

Firefly luciferase

Mutation

Contains human GFAP promoter fragment (-2163 to +…

Promoter

GFAP promoter

Available Since

Sept. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pm-iATPSnFR1.0

Plasmid

#102548

Purpose

membrane displayed ATP sensor for mammalian cells

Depositor

Baljit Khakh

Article

Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

Insert

iATPSnFR1.0

Expression

Mammalian

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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TK4-EGFP

Plasmid

#239047

Purpose

PiggyBac plasmid for stable transgene expression during human pluripotent stem cell differentiation

Depositor

Marius Wernig

Article

Uenaka et al Cell Stem Cell. 2026 Feb 13:S1934-5909(2

Inserts

EGFP
puroR

Expression

Mammalian

Promoter

CAG and EF1a

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-Hylight-T152E

Plasmid

#193448

Purpose

A binding-dead fluorescent biosensor for fructose 1,6-bisphosphate (FBP) for negative control experiments (mammalian expression vector)

Depositor

Richard Goodman

Article

Koberstein et al Proc Natl Acad Sci U S A. 2022 Aug 2;119

Insert

Binding-Dead Hylight

Expression

Mammalian

Mutation

Threonine 67 (ACT) mutated to a Glutamic Acid (GA…

Promoter

CMV

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-JEDI-2P-WPRE (AAV1)

Viral Prep

#179460-AAV1

Purpose

Ready-to-use AAV1 particles produced from pAAV-EF1a-DIO-JEDI-2P-WPRE (#179460). In addition to the viral particles, you will also receive purified pAAV-EF1a-DIO-JEDI-2P-WPRE plasmid DNA. EF1a-driven, Cre-dependent expression of voltage indicator JEDI-2P. These AAV preparations are suitable purity for injection into animals.

Depositor

Francois St-Pierre

Article

Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

Promoter

EF1a

Tags

cEGFP (Cre-dependent)

Available Since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only
pLVX-NPC1(WT)-FLAG

Plasmid

#164972

Purpose

Lentiviral vector for NPC1 expression in mammalian cells

Depositor

Roberto Zoncu

Article

Davis et al Dev Cell. 2020 Dec 7. pii: S1534-5807(20

Insert

NPC1 (NPC1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

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SPLICS PM-ER Short P2A

Plasmid

#164112

Purpose

Detect the short Plasma membrane-Endoplasmic reticulum contact

Depositor

Marisa Brini, Tito Calì

Article

Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

Insert

Split YFP PM-ER Short P2A

Expression

Mammalian

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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