We narrowed to 17,418 results for: She

1. pBEST-OR2-OR1-Pr-araC, pBAD-TetR, pBAD-TetO1-deGFP-ssrA

   Plasmid

   #45789

   Depositor

   Richard Murray

   Inserts

   deGFP
   Tetracycline repressor
   araC
   

   Use

   Synthetic Biology

   Tags

   ssrA degradation tag

   Expression

   Bacterial

   Mutation

   Promoter

   OR2-OR1-Pr (bacteriophage Lambda with one mutatio…

   Available since

   April 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMSCV_puro_41584

   Plasmid

   #111630

   Purpose

   Complementation of Dicer_KO mESCs with wild type human DICER isoform

   Depositor

   Constance Ciaudo

   Insert

   FLAG-hsDicer (DICER1 Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-CMV-FLEX-SaCas9-U6-sgTh

   Plasmid

   #159901

   Purpose

   Mutagenesis of Th

   Depositor

   Larry Zweifel

   Insert

   Th (Th Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pXP220

   Plasmid

   #26832

   Depositor

   Suzanne Sandmeyer, Nancy DaSilva

   Article

   Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Dec. 8, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. LRRK2_1867_2136

   Plasmid

   #40408

   Depositor

   Michael J Fox Foundation MJFF

   Insert

   LRRK2 (LRRK2 Human)
   

   Use

   Baculovirus

   Tags

   GST-Tev and HisTev

   Expression

   Mutation

   WT aa1867_2136

   Promoter

   polH

   Available since

   Oct. 1, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pEMB018 / LRRK2_BC_1847_2350

   Plasmid

   #40533

   Depositor

   Michael J Fox Foundation MJFF

   Insert

   LRRK2 (LRRK2 Human)
   

   Use

   Baculovirus

   Tags

   6xHis

   Expression

   Mutation

   WT; aa1847_2350

   Promoter

   polH

   Available since

   Oct. 1, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. CUP1-∆ssCPY*-mGFP in pRS315

   Plasmid

   #221158

   Purpose

   Fluorescent reporter for cytoplasmic aggregates under LEU2 selection marker

   Depositor

   Mark Leake

   Insert

   Carboxypeptidase Y (PRC1 Budding Yeast)
   

   Use

   Tags

   mGFP

   Expression

   Yeast

   Mutation

   Deletion signal peptide, G255A mutation

   Promoter

   Available since

   Nov. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. CUP1-∆ssCPY*-mScarletI in pRS315

   Plasmid

   #221159

   Purpose

   Fluorescent reporter for cytoplasmic aggregates under LEU2 selection marker

   Depositor

   Mark Leake

   Insert

   Carboxypeptidase Y (PRC1 Budding Yeast)
   

   Use

   Tags

   mScarletI

   Expression

   Yeast

   Mutation

   Deletion signal peptide, G255A mutation

   Promoter

   Available since

   Nov. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDONR221-TMPRSS11D

   Plasmid

   #158447

   Purpose

   Gateway Cloning compatible entry vector for the human TMPRSS11D gene.

   Depositor

   Alejandro Chavez, Sho Iketani

   Insert

   TMPRSS11D (TMPRSS11D Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET50_MDA5∆2CARD

    Plasmid

    #216804

    Purpose

    Expresses melanoma differentiation-associated protein 5 lacking its 2 CARD domains

    Depositor

    Sun Hur

    Insert

    melanoma differentiation-associated protein 5 delta 2 CARD domains (IFIH1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    ∆1-287

    Promoter

    Available since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. GLI2 his

    Plasmid

    #87097

    Purpose

    expresses GLI2 in mammalian cells

    Depositor

    Martin Fernandez-Zapico

    Insert

    GLI2 (GLI2 Human)
    

    Use

    Tags

    6x His, T7 tag, and Xpress tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLVX-CMV100

    Plasmid

    #110718

    Purpose

    Lentiviral system for reduced ectopic expression.

    Depositor

    Kevin Dean, Reto Fiolka

    Article

    Dean et al Biophys J. 2016 Mar 29;110(6):1456-65. d

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGEX6P1-N-Strep2

    Plasmid

    #119758

    Purpose

    Allows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal Strep-II tag.

    Depositor

    Martin Reijns, Andrew Jackson

    Type

    Empty backbone

    Use

    Tags

    GST and Strep-tag II fusion tag

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pT7-V5-SBP-C1-HsRRP6

    Plasmid

    #64916

    Purpose

    expresses V5- and SBP-tagged RRP6 in mammalian cells

    Depositor

    Elisa Izaurralde

    Insert

    RRP6 (EXOSC10 Human)
    

    Use

    Tags

    V5 and SBP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCMV-ABE-P48R-UGI

    Plasmid

    #161817

    Purpose

    Expresses ABE-P48R-UGI in mammalian cells

    Depositor

    Sangsu Bae

    Insert

    bpNLS-TadA7.10(P48R)-32AA linker-hSpCas9n(D10A)-10AA linker-UGI-10AA linker-UGI-bpNLS-P2A-EGFP-NLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A in S. pyogenes Cas9, TadA mutations describe…

    Promoter

    Available since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. JMJD3

    Plasmid

    #39069

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    JMJD3 (KDM6B Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pTRIPZ (M)-HT-Cbx4

    Plasmid

    #82513

    Purpose

    Lentivirus vector. Expresses Halotag-Cbx4 fusion proteins in mammalian cells.

    Depositor

    Xiaojun Ren

    Insert

    Chromobox Homolog 4 (Cbx4 Mouse)
    

    Use

    Lentiviral

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    Dec. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pQE80L-IL2 C125S

    Plasmid

    #104348

    Purpose

    Expresses human IL-2 (C125S)

    Depositor

    Alexander McLellan

    Insert

    Human IL-2 (C125S) (IL2 Human)
    

    Use

    Tags

    MRGSHHHHHGS-tag

    Expression

    Bacterial

    Mutation

    C125S mutation (TGT -> TCC)

    Promoter

    T5

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HaloTag-G protein beta-1

    Plasmid

    #67016

    Purpose

    G protein beta-1 tagged with HaloTag on amino terminus

    Depositor

    Catherine Berlot

    Insert

    HaloTag-G protein beta-1 (GNB1 Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG/HA-hBIG1

    Plasmid

    #226253

    Purpose

    Expresses N-terminally HA-tagged hBIG1 in mammalian cells

    Depositor

    Hye-Won Shin

    Insert

    BIG1 (ARFGEF1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pXP218

    Plasmid

    #26831

    Depositor

    Suzanne Sandmeyer, Nancy DaSilva

    Article

    Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Human-Dysferlin-v1

    Plasmid

    #60217

    Purpose

    Human dysferlin exon 1 variant (accession #DQ267935) cDNA in pDONR221 plasmid which can be used as an entry vector for Invitrogen’s gateway system.

    Depositor

    Jain Foundation

    Insert

    Dysferlin (DYSF Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. AK2

    Plasmid

    #38829

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    AK2 (AK2 Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

24. SOX9-T2A-NLS-tdTomato-PGK-Puro

    Plasmid

    #89993

    Purpose

    Donor template for generation of SOX9-ntdTomato reporter cell lines

    Depositor

    Timo Otonkoski

    Insert

    SOX9-T2A-NLS-TdTomato-PGK-Puro (SOX9 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pTXB1-sfGFP

    Plasmid

    #51562

    Purpose

    Expression of superfolder GFP for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backbone)

    Depositor

    Francisco Malagon, Sankar Adhya

    Insert

    superfolder GFP
    

    Use

    Tags

    Intein-Chiting Binding Domanin

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET28-6His-TEV-Nsp14

    Plasmid

    #154954

    Purpose

    Expresses Nsp14 from SARS-CoV-2 with a N-terminal TEV-protease cleavable 6His tag in E. coli

    Depositor

    Justin Kollman

    Insert

    6His-TEV-Nsp14 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. FLAG-hSRF (#482)

    Plasmid

    #78343

    Purpose

    Overexpression of human SRF ORF with a N-terminal FLAG tag

    Depositor

    Matthew Alexander, Louis Kunkel

    Insert

    SRF (SRF Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

28. AK1

    Plasmid

    #39134

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    AK1 (AK1 Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

29. AK5

    Plasmid

    #39136

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    AK5 (AK5 Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 7, 2013

    Availability

    Academic Institutions and Nonprofits only

30. CLK1

    Plasmid

    #39149

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    CLK1 (CLK1 Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pETSXM2-pLacI-GFP

    Plasmid

    #174617

    Purpose

    Bacterial expression of GFP.

    Depositor

    I-Son Ng

    Article

    I-Son Ng et al Bioresour. Bioprocess. 5, 35 (2018)

    Insert

    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLacI

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pKA-141

    Plasmid

    #79832

    Purpose

    Expresses RpBphP1-mCherry

    Depositor

    Vladislav Verkhusha

    Article

    Kaberniuk et al Nat Methods. 2016 May 9. doi: 10.1038/nm

    Insert

    BphP1-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMVd1

    Available since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pXP118

    Plasmid

    #26825

    Depositor

    Suzanne Sandmeyer, Nancy DaSilva

    Article

    Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. p5E-elavl3

    Plasmid

    #72640

    Purpose

    Gateway p5E 5'entry clone with elavl3 (HuC) enhancer/promoter for pan-neuronal expression in zebrafish

    Depositor

    Joshua Bonkowsky

    Insert

    elavl3 enhancer (elavl3 Zebrafish)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCAG-GluA2-Fab-LC

    Plasmid

    #230063

    Purpose

    Expresses light chain Fab fragment of anti-GluA2. Can be used with heavy chain to produce Fab in HEK cells.

    Depositor

    Matthew Kennedy

    Insert

    Anti-GluA2 Fab light chain fragment
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken beta actin (CAG)

    Available since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. DUSP16

    Plasmid

    #38918

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    DUSP16 (DUSP16 Human)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

37. CFP-GIV-WT

    Plasmid

    #71544

    Purpose

    Mammalian expression of The CFP-tagged GIV-WT biosensors

    Depositor

    Pradipta Ghosh

    Insert

    GIV/Girdin (CCDC88A Human)
    

    Use

    Tags

    CFP

    Expression

    Mutation

    Promoter

    Available since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. BRD3

    Plasmid

    #38941

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    BRD3 (BRD3 Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

39. pXZ13lacZalpha

    Plasmid

    #160229

    Purpose

    places LacZalpha unit between Esp3I sites for better cutting of plasmid and ability to distinguish recombinants as white colonies when plated on Xgal plates

    Depositor

    Ian Duncan

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. JMJD5

    Plasmid

    #39113

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    JMJD5 (KDM8 Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only