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Showing: 3501 - 3520 of 23872 results

  1. pCA528 IST1 L328D (316-366)(C-Cys)

    Plasmid
    #193035
    Purpose
    Bacterial expression plasmid for IST1 (316-366) with C-terminal Cysteine for fluorescence polarization binding assays. Contains L328D mutation that diminishes binding to MIT domains.
    Depositor
    Wesley Sundquist
    Article
    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75
    Insert
    IST1 (IST1 Human)
    Tags
    6xHis-Sumo
    Expression
    Bacterial
    Mutation
    L328D, Residues 316-366, Non-native C-terminal Cy…
    Promoter
    T7
    Available Since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    193035-plasmid-map-sequence-id-379007

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  2. pCA528 IST1 L355A (316-366)(C-Cys)

    Plasmid
    #193036
    Purpose
    Bacterial expression plasmid for IST1 (316-366) with C-terminal Cysteine for fluorescence polarization binding assays. Contains L355A mutation that diminishes binding to MIT domains.
    Depositor
    Wesley Sundquist
    Article
    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75
    Insert
    IST1 (IST1 Human)
    Tags
    6xHis-Sumo
    Expression
    Bacterial
    Mutation
    L355A, Residues 316-366, Non-native C-terminal Cy…
    Promoter
    T7
    Available Since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    193036-plasmid-map-sequence-id-379013

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  3. pCHMP4B-mut-NS3-Green

    Plasmid
    #223531
    Purpose
    Mammalian expression of CHMP4B fused to the hepatitis C virus protease NS3 through a short linker containing mutated NS3 cleavage site, along with a green fluorescent protein.
    Depositor
    Winfried Weissenhorn
    Article
    Wang et al Viruses. 2023 Nov 22;15(12):2289. doi: 1
    Insert
    CHMP4B (CHMP4B Human)
    Tags
    NS3 Hepatitis C protease and mNeonGreen
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    223531-plasmid-map-sequence-id-443356

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  14. pLPC-JAG1-mVenus

    Plasmid
    #171170
    Purpose
    Retroviral vector for CMV promoter driven expression of JAG1 (Notch pathway) fused to mVenus fluorescent protein (to be used in conjunction with Phoenix packaging cells).
    Depositor
    Masashi Narita
    Article
    Hoare et al Nat Cell Biol. 2016 Sep;18(9):979-92. do
    Insert
    JAG1 (JAG1 Human)
    Use
    Retroviral
    Tags
    mVenus
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    171170-plasmid-map-sequence-id-339658

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  15. pCMV(MinDis).iGluSnFR

    Plasmid
    #41732
    Purpose
    Single-wavelength extracellular glutamate sensor constructed from E. coli Gltl and cpGFP. Membrane-displayed. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:
    Insert
    GltI based Glutamate Biosensor
    Use
    Glutamate biosensor
    Tags
    Myc, PDGFR transmembrane helix (aa 513-561), and …
    Expression
    Mammalian
    Mutation
    cpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET i…
    Promoter
    CMV
    Available Since
    Jan. 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    41732-plasmid-map-sequence-id-210671

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  17. pmCherry-C1 R62D actin-3XNLS P2A mCherry

    Plasmid
    #58477
    Purpose
    Expresses nuclear-targeted non-polymerizing R62D mutant of human actin, with an mCherry expression reporter after a P2A protease cleavage site, on a CMV promoter
    Depositor
    Dyche Mullins
    Article
    Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife
    Inserts
    beta actin (ACTB Human)
    3XNLS
    P2A cleavage site
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Changed Arginine 62 to Aspartic Acid
    Promoter
    CMV
    Available Since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    58477-plasmid-map-sequence-id-90386

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Showing: 3501 - 3520 of 23872 results