We narrowed to 10,980 results for: SEC
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Plasmid#148939PurposeInsect Expression of DmGIGYF-WFFtoAAADepositorInsertDmGIGYF-WFFtoAAA (Gyf Fly)
ExpressionInsectMutationone silent mutation compared to the sequence give…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-Dm4EHP-W85A_AH
Plasmid#148917PurposeInsect Expression of Dm4EHP-W85ADepositorInsertDm4EHP-W85A (4EHP Fly)
ExpressionInsectMutation4 silent mutations compared to the sequence given…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAcGHLT-B-DDB1
Plasmid#48638PurposeExpresses Full Lenght DDB1 in Insect Cells (ampR)DepositorInsertDDB1 (DDB1 Human)
ExpressionInsectAvailable SinceOct. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_ManII-SBP-mCherry
Plasmid#65253Purposesynchronize trafficking of ManII from the ER (RUSH system)DepositorInsertStreptavidin-KDEL and truncated Mannosidase II
TagsStreptavidin Binding Protein (SBP) and mCherryExpressionMammalianMutationcontains only amino acids 1 to 116 of ManIIPromoterCMVAvailable SinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-DIO-ChR2-TdTomato
Plasmid#166600PurposeEncodes Cre-dependent ChR2-TdTomato under control of the TREDepositorInsertChR2-TdTomato
UseAAVMutationH134RPromotertetracycline response elementAvailable SinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
mH2A1.1-CT-GFP
Plasmid#45167DepositorAvailable SinceJuly 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
FL-mCherry
Plasmid#78862PurposeDULIP negative control (prey) to exclude unspecific background bindingDepositorInsertmCherry
UseExpression vectorTagsFirefly luciferase, V5-tagExpressionMammalianPromoterCMVAvailable SinceJuly 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-CHD7
Plasmid#170141PurposeExpresses CHD7 in insect cellsDepositorInsertchromodomain helicase DNA binding protein 7 (CHD7 Human)
Tags6xHis and FLAGExpressionInsectAvailable SinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-28a-His6-SUMO-VAMP2
Plasmid#135551PurposeExpress mouse VAMP2 in E. coli BL21, purified via HisPur Ni-NTA ResinDepositorInsertVAMP2
Tags6xHis tag, thrombin recognition and cleavage site…ExpressionBacterialMutationNo ATG start codonPromoterT7 promoterAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDR111_Bgal no NLS
Plasmid#188397PurposeExpresses beta-galactosidase via Phyper-spank which is optimized for Bacillus subtilis. There is a secretion peptide (PhoD) that is fused to beta-galactosidase.DepositorInsertlacZ
TagsPhoD secretion peptideExpressionBacterialPromoterPhyper spankAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC57-GFP-SFPQ-RT
Plasmid#97090PurposeEncodes a HR repair template for knock-in of an EGFP insert that fused to the 5'-end of human SFPQ gene, without disturbing its promoter. Best used with px330-GFP-SFPQ vector.DepositorInsertEGFP-SFPQ-RT (SFPQ Human)
UseCRISPRAvailable SinceJuly 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
ShadowG-SUMO1
Plasmid#175239PurposeBacterial expression and purification, conjugatable SUMO for SUMOylationDepositorAvailable SinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFRTpsiCHECK
Plasmid#154454PurposeExpression of renilla luciferase and firefly luciferaseDepositorInsertRenilla luciferase, firefly luciferase
Available SinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAB076i3
Plasmid#79198PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI-SH2ABL1
ExpressionBacterialPromoterPlacZ-opt (OR1); Ppro1Available SinceApril 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
mH2A1.1-CT-MYC
Plasmid#45166DepositorAvailable SinceJuly 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pIZ-Flag6His-Siwi
Plasmid#50560PurposeExpresses FLAG, His tagged Siwi in silkworm cell line BmN4 or insect cellsDepositorAvailable SinceMarch 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-DIO-APEX2NES
Plasmid#79907PurposeAdeno-associated virus plasmid, Cre-dependent FLEX switch, APEX2NESDepositorInsertAPEX2NES
UseAAV and Cre/LoxTagsV5ExpressionMammalianAvailable SinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Flag-gp130dimer
Plasmid#174394PurposeCytosolic domain of gp130 with C-C linker and Leu zipper for constitutive dimerization and activation (from Stuhlmann-Laeisz et al. 2006).DepositorInsertIL6ST cytosolic domain with leucine zipper & C-C linker
TagsFlagExpressionMammalianAvailable SinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMMB67EH-Spy
Plasmid#90102PurposeIPTG-inducible CFP/YFP FRET-based biosensor for cyclic-di-GMP measurement using YcgR binding site.DepositorAvailable SinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFSW GAP43-jRGECO1a P2A Syp-Halo
Plasmid#185565PurposeExpression of axonally-targeted jRGECO1a, a red Ca2+ sensor, along with synaptophysin-HaloTag in neuronal cells, separated by a P2A sequenceDepositorInsertGAP43-jRGECO1a P2A Syp-Halo
UseLentiviralTagsHaloTagAvailable SinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only