We narrowed to 9,869 results for: SUB;

1. pCK-Drosha(UniProt)-FLAG

   Plasmid

   #164690

   Purpose

   Express Drosha-FLAG in mammalian cells

   Depositor

   David Bartel

   Article

   Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

   Insert

   Drosha (DROSHA Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Sema6d.4-AP-His

   Plasmid

   #72044

   Purpose

   Expresses the extracellular region of the Sema6D, isoform 4 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Sema6d.4 (Sema6d Mouse)
   

   Use

   Tags

   AP-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRT498-OtUBD(V203D)

   Plasmid

   #190090

   Purpose

   Expresses OtUBD(V203D) mutant that is unable to bind ubiquitin with an TEV-cleavable N-terminal 6xHis-MBP tag as well as an engineered N-terminal cysteine in E. coli

   Depositor

   Mark Hochstrasser

   Article

   Zhang et al PLoS Biol. 2022 Jun 30;20(6):e3001501. d

   Insert

   OtUBD(V203D)
   

   Use

   Tags

   6xHis and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. PZac2.1 hSyn1-HA-BioID2-SAPAP3

   Plasmid

   #176857

   Purpose

   Expresses SAPAP3 protein fused with BioID2 at the N-terminus in neurons

   Depositor

   Baljit Khakh

   Article

   Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

   Insert

   HA-BioID2-SAPAP3
   

   Use

   AAV

   Tags

   HA

   Expression

   Bacterial

   Mutation

   Promoter

   hSyn1

   Available since

   April 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEJS887_pTetR-P2A-BFPnls/sgAlpha

   Plasmid

   #108651

   Purpose

   Alpha satellite repeats targeting Spy sgRNA under U6 promoter

   Depositor

   Erik Sontheimer

   Article

   Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

   Inserts

   Alpha satellite repeats targeting sgRNA
   TetR-P2A-BFP
   

   Use

   CRISPR and Lentiviral

   Tags

   No

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and hPGK

   Available since

   May 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. GST-PBD

   Plasmid

   #60880

   Purpose

   Bacterially expressed GST-tagged human polo-like kinase 1 PBD AA365-653

   Depositor

   Trina Schroer

   Article

   Yeh et al EMBO J. 2013 Apr 3;32(7):1023-35. doi: 1

   Insert

   Polo-like kinase 1 (PLK1 Human, BC01486)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 26, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pGM8

   Plasmid

   #220166

   Purpose

   Expresses a reporter for an alpha-helical OMM protein

   Depositor

   Jonathan Weissman

   Article

   Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

   Insert

   TSPO (TSPO Human)
   

   Use

   Lentiviral

   Tags

   GFP11

   Expression

   Mutation

   Promoter

   Available since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-GNPTAB

   Plasmid

   #78108

   Purpose

   Mammalian expression of human GNPTAB with C terminal myc tag

   Depositor

   Thomas Braulke

   Article

   Franke et al J Biol Chem. 2013 Jan 11;288(2):1238-49.

   Insert

   GNPTAB (GNPTAB Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 19, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pScalps_puro_mIL-2Rα

   Plasmid

   #59917

   Purpose

   Expresses mouse interleukin-2 receptor alpha chain

   Depositor

   Silvia Monticelli

   Article

   Deho' et al J Immunol. 2014 Sep 1;193(5):2196-206. d

   Insert

   interleukin 2 receptor alpha (Il2ra Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pCK-Drosha(UniProt)-FLAG-mEGFP

    Plasmid

    #164691

    Purpose

    Express Drosha-FLAG-mEGFP in mammalian cells

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Insert

    Drosha (DROSHA Human)
    

    Use

    Tags

    FLAG and mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-EF1a-DIO-H2B.APEX-P2A-EGFP

    Plasmid

    #182825

    Purpose

    Cre-dependent expression of H2B-APEX fusion

    Depositor

    Yevgenia Kozorovitskiy

    Article

    Dumrongprechachan et al Nat Commun. 2021 Aug 11;12(1):4855. doi:

    Insert

    H2B.APEX-P2A-EGFP
    

    Use

    AAV

    Tags

    EGFP reporter (P2A)

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5-Flag-Narg2

    Plasmid

    #49247

    Purpose

    mammalian expression of flag-tagged human Narg2

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

    Insert

    Narg2 (NARG2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

13. 3xFlag-HaloTag-SUZ12 HDR - pDY082

    Plasmid

    #171110

    Purpose

    Homology directed repair plasmid for generating HaloTag-SUZ12 cells

    Depositor

    Thomas Cech

    Article

    Youmans et al Genes Dev. 2018 Jun 1;32(11-12):794-805.

    Insert

    SUZ12 (SUZ12 Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. p11d-tRPA-32Ala9

    Plasmid

    #102616

    Purpose

    Expression of human RPA with an alanine substituted phosphorylation domain (with the following mutations in RPA32 :S8,11-13,23,29,33,39A,T21A).

    Depositor

    Marc Wold

    Inserts

    RPA1 (RPA1 Human)
    RPA3 (RPA3 )
    RPA2 (RPA2 )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    with an alanine substituted phosphorylation domain

    Promoter

    T7

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. Cav2.2 HA pcDNA3

    Plasmid

    #206051

    Purpose

    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II

    Depositor

    Annette Dolphin

    Article

    Cassidy et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCDNA3_HMOX1-V5-APEX2

    Plasmid

    #85823

    Purpose

    Expressed C-term tagged APEX2 on HMOX1 in mammalian cell

    Depositor

    Hyun-Woo Rhee

    Article

    Lee et al Cell Rep. 2016 May 11. pii: S2211-1247(1

    Insert

    HMOX1-V5-APEX2 (HMOX1 Synthetic, Human)
    

    Use

    Tags

    APEX2 and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-F

    Available since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pECE M2-SNX17 WT

    Plasmid

    #69811

    Purpose

    Expresses M2-SNX17 in mammalian cells

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    Sorting nexin-17 (SNX17 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    SV40 early promoter

    Available since

    May 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKG-GW1

    Plasmid

    #203163

    Purpose

    Yeast expression vector; To express proteins under control of TDH3 promoter and terminator using leucine selection

    Depositor

    William Sessa

    Article

    Park et al Cell Metab. 2014 Sep 2;20(3):448-57. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRRLSIN.cPPT.PGK.MD(ND2bHLH).WPRE

    Plasmid

    #122055

    Purpose

    Lentiviral expression of a MyoD chimeric protein substituted with the NeuroD2 bHLH domain

    Depositor

    Stephen Tapscott

    Article

    Fong et al Cell Rep. 2015 Mar 31;10(12):1937-46. do

    Insert

    MD(ND2bHLH) (Myod1, Neurod2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    MyoD chimeric protein substituted with the NeuroD…

    Promoter

    Available since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFP-C1-BBS5

    Plasmid

    #218714

    Purpose

    Expresses N-terminally EGFP-tagged BBS5 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

    Insert

    BBS5 (BBS5 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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