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pUBC-SV40NLS-Pfv-Sapphire-PURO

Plasmid

#203654

Purpose

Mammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC promoter, with a puromycin selection marker.

Depositor

Liam Holt

Article

Shu T et et al bioRxiv 2022

Insert

PfV

Use

Lentiviral

Tags

Sapphire

Available Since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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pZmUBQ:GW

Plasmid

#132358

Purpose

Expression of CDS from the Zea mays ubiquitin promotor

Depositor

Paul Schulze-Lefert

Article

Saur et al Plant Methods. 2019 Oct 24;15:118. doi:

Type

Empty backbone

Tags

YFP

Expression

Plant

Promoter

pZmUBQ

Available Since

Oct. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pCfB6364

Plasmid

#106151

Purpose

EasyCloneYALI system-based yeast integrative vector expressing Cas9 carrying D-serine marker, integration into Yarrowia lipolytica ku70 locus, amp resistance

Depositor

Irina Borodina

Article

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Insert

Cas9

Expression

Yeast

Available Since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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Golgi-ABKAR

Plasmid

#61507

Purpose

Expression of AMPK biosensor targeted to the Golgi

Depositor

Takanari Inoue, Jin Zhang

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

AMPK Biosensor

Tags

Golgi-targeting signal (see comments)

Expression

Mammalian

Promoter

CMV

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
3396 pBM272 Bax

Plasmid

#8770

Depositor

Stanley Korsmeyer

Article

Gross et al Mol Cell Biol 2000 May;20(9):3125-36.

Insert

Bax (Bax Mouse)

Expression

Yeast

Available Since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only
pLIX403 RNaseH1

Plasmid

#237695

Purpose

Inducible lentiviral expression of RNaseH1

Depositor

David Pellman

Article

Tang et al Nature. 2022 Jun;606(7916):930-936. doi:

Insert

RNaseH1 (RNASEH1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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Yellow Cameleon-Nano65/pcDNA3

Plasmid

#51965

Purpose

mammalian expression of Yellow Cameleon-Nano65, an ultrasensitive Ca2+ indicator with Kd = 65 nM

Depositor

Takeharu Nagai

Article

Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

Insert

Yellow Cameleon-Nano65

Expression

Mammalian

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
HS-H3-GFP

Plasmid

#8698

Depositor

Kami Ahmad

Article

Ahmad et al Mol Cell 2002 Jun;9(6):1191-200.

Insert

H3 (His3:CG31613 Fly)

Tags

GFP

Expression

Insect

Available Since

Aug. 15, 2005

Availability

Academic Institutions and Nonprofits only

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pLV-GFP-Sec61β

Plasmid

#186597

Purpose

Third generation lentiviral vector expressing GFP-Sec61β

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Sec61β (SEC61B Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pCherryGFP-CoV2-Inframe-stop

Plasmid

#177620

Purpose

Dual fluorescent protein reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. GFP is in-frame with mCherry. Thus GFP translation is prevented by stop codon within FSE (negative control).

Depositor

Junjie Guo

Article

Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11

Insert

SARS-CoV-2 FSE

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2+ centrin-mKate2

Plasmid

#105941

Purpose

in vitro transcription, zf centrin N-terminally tagged with mkate2

Depositor

Caren Norden

Article

Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

Insert

Centrin

Tags

mKate2

Expression

Bacterial

Available Since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pJZC518

Plasmid

#62277

Purpose

Yeast dCas9 expression plasmid

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

dCas9

Expression

Yeast

Mutation

Has mutations in the RuvC1 and HNH domains to ren…

Promoter

pTdh3

Available Since

March 17, 2015

Availability

Academic Institutions and Nonprofits only

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Filamin-M-stFRET

Plasmid

#61105

Purpose

Expression of Filamin with internal stFRET inserted to measure mechanical stress in real time

Depositor

Fred Sachs

Article

Meng et al FEBS J. 2008 Jun;275(12):3072-87. doi: 1

Insert

filamin-stFRET

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2015

Availability

Academic Institutions and Nonprofits only

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pBAD-LSSmGFP

Plasmid

#186542

Purpose

LSSmGFP fluorescent protein (ex/em = 400/510 nm): bacterial expression plasmid

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

LSSmGFP

Tags

6xHis

Expression

Bacterial

Mutation

hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K

Promoter

araB

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-FLInChR-mVenus

Plasmid

#119298

Purpose

FLinChR is the fusion signal sequence and transmembrane (TM) domain of Neurexin 1B-delta to ChR2E123T/T159C for inversion ChR2 to an optogenetic inhibitor

Depositor

Alla Karpova

Article

Brown et al Cell. 2018 Oct 16. pii: S0092-8674(18)31

Insert

Nx1BTM-FCS-ChR2ET/TC-mVenus

Use

AAV

Tags

mVenus

Promoter

CAG

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+-pHlash

Plasmid

#61552

Purpose

Expression of pHlash in mammalian cells

Depositor

Carl Johnson

Article

Zhang et al PLoS One. 2012;7(8):e43072. doi: 10.1371

Insert

pHlash

Tags

NA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 5, 2015

Availability

Academic Institutions and Nonprofits only
6375 H10Ydj1

Plasmid

#1234

Depositor

Susan Lindquist

Article

Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

Insert

Ydj1 (YDJ1 Budding Yeast)

Tags

his tag

Expression

Bacterial

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pAAV.GFAP.iGluSnFR3.v857.PDGFR

Plasmid

#178336

Purpose

Fluorescent reporter for glutamate, third generation, variant 857. iGluSnFR3.v857

Depositor

Kaspar Podgorski

Article

Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

Insert

pAAV GFAP iGluSnFR3 v857.PDGFR

Use

AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

Tags

IgK-chain, Myc epi tag, and PDGFR TM Domain

Expression

Mammalian

Available Since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pSEVA221TadA*-T7RNAP

Plasmid

#167980

Purpose

Expresses fusion protein TadA*-T7RNAP in E. coli

Depositor

Luis Ángel Fernández

Article

Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

Insert

TadA*-T7RNAP

Tags

T7 tag and T7RNAP

Expression

Bacterial

Promoter

TetR-Ptet

Available Since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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LDB-3-p65

Plasmid

#158984

Purpose

Expresses LDB-3 fused with a C-terminal p65 transcriptional activation domain in mammalian cells

Depositor

Liangcai Gu

Article

Huang et al ACS Synth Biol. 2020 Nov 12. doi: 10.102

Insert

LDB-3-p65

Use

Lentiviral

Tags

Myc tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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