We narrowed to 150,552 results for: ins

1. pAc5.1B-lambdaN-DmPABPC1-del364-550-siRNAres-V5His6_L

   Plasmid

   #146864

   Purpose

   Insect Expression of DmPABPC1_del364-550-siRNAres

   Depositor

   Elisa Izaurralde

   Article

   Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

   Insert

   DmPABPC1_del364-550-siRNAres (pAbp Fly)
   

   Expression

   Insect

   Mutation

   one non silent mutation G26S, and five silent mut…

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL3-pSMANTIS_SMAD-FOS_Mutant

   Plasmid

   #194188

   Purpose

   Includes the promoter (1kb) of SMTS with mutated SMAD/FOS binding site

   Depositor

   Ralf Brandes, Matthias Leisegang

   Article

   Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

   Insert

   SMANTIS promoter (1kb) (SMANTIS Human)
   

   Use

   Luciferase

   Mutation

   mutated SMAD/FOS Site, Region 3-15nt of 1000nt

   Promoter

   SMANTIS promoter (1kb), mutated

   Available Since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL3-pSMANTIS_STAT1-2_Mutant

   Plasmid

   #194189

   Purpose

   Includes the promoter (1kb) of SMTS with mutated STAT1/2 binding site

   Depositor

   Ralf Brandes, Matthias Leisegang

   Article

   Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

   Insert

   SMANTIS promoter (1kb) (SMANTIS Human)
   

   Use

   Luciferase

   Mutation

   mutated STAT1/2 site, Region 653-673nt of 1000nt

   Promoter

   SMANTIS promoter (1kb), mutated

   Available Since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR223 ATG3 C264A

   Plasmid

   #129293

   Purpose

   Gateway entry clone encoding human ATG3 C264A (catalytic inactive)

   Depositor

   Robin Ketteler

   Article

   Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

   Insert

   Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
   

   Use

   Gateway entry vector / entry clone

   Mutation

   changed Cysteine 264 to Alanine

   Available Since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR223 ATG3 allKR

   Plasmid

   #129295

   Purpose

   Gateway entry clone encoding human ATG3 with all 22 lysine residues mutated to arginine

   Depositor

   Robin Ketteler

   Article

   Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

   Insert

   Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
   

   Use

   Gateway entry vector / entry clone

   Mutation

   changed all 22 Lysine residues to Arginine

   Available Since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV 3xFLAG-ATG3 allKR

   Plasmid

   #129298

   Purpose

   Expresses pCMV 3xFLAG-ATG3 allKR (all lysines mutated to arginine) in mammalian cells

   Depositor

   Robin Ketteler

   Article

   Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

   Insert

   Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   changed all 22 Lysine residues to Arginine

   Promoter

   CMV

   Available Since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV 3xFLAG-ATG3 1-13 KR

   Plasmid

   #129299

   Purpose

   Expresses pCMV 3xFLAG-ATG3 1-13 KR (first thirteen lysines mutated to arginine) in mammalian cells

   Depositor

   Robin Ketteler

   Article

   Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

   Insert

   Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   changed first 13 Lysine residues to Arginine

   Promoter

   CMV

   Available Since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV 3xFLAG-ATG3 14-22 KR

   Plasmid

   #129300

   Purpose

   Expresses pCMV 3xFLAG-ATG3 14-22 KR (last nine lysines mutated to arginine) in mammalian cells

   Depositor

   Robin Ketteler

   Article

   Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

   Insert

   Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   changed last 9 Lysine residues to Arginine

   Promoter

   CMV

   Available Since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV 3xFLAG-ATG3 K295R

   Plasmid

   #129302

   Purpose

   Expresses pCMV 3xFLAG-ATG3 K295R in mammalian cells

   Depositor

   Robin Ketteler

   Article

   Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

   Insert

   Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   changed Lysine 295 to Arginine

   Promoter

   CMV

   Available Since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-30a(+)-Syk-tSH2-FX

    Plasmid

    #111272

    Purpose

    express murine Syk tandem SH2 domains (Ser 8 to Gln 264), with interdomain A (Phe 119 to His 162) substituted by a flexible 20-amino-acid linker (GGS)3GS(GGS)3, in E.coli strain Rosetta 2 (DE3)

    Depositor

    Carol Post

    Article

    Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5

    Insert

    murine Syk tandem SH2 domains (Ser 8 to Gln 264), with interdomain A (Phe 119 to His 162) substituted by a flexible 20aa linker (Syk Mouse)
    

    Expression

    Bacterial

    Mutation

    interdomain A (Phe 119 to His 162) substituted by…

    Promoter

    T7 promoter

    Available Since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. NKP9 pmRFP-N2 (719)

    Plasmid

    #61999

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

    Insert

    C17orf62 (CYBC1 Human)
    

    Tags

    mRFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

12. NKP40 RFP (794)

    Plasmid

    #62006

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

    Insert

    C17orf32 (C17orf32 Human)
    

    Tags

    mRFP

    Expression

    Mammalian

    Promoter

    pgk

    Available Since

    Jan. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pCMV-T7-SpRY-P2A-EGFP (RTW4830)

    Plasmid

    #139989

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9 variant named SpRY with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

    Promoter

    CMV and T7

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. p3xFLAG SARS-CoV2 RdRp.co

    Plasmid

    #154008

    Purpose

    Expression of codon-optimized SARS-CoV2 Nsp12 (RdRp) in mammalian cells

    Depositor

    Patrick Moore

    Article

    Meng et al J Med Virol. 2023 Jan;95(1):e28246. doi:

    Insert

    Codon-optimized SARS-CoV2 Nsp12 from ORF1ab (ORF1ab Synthetic)
    

    Tags

    3xFLAG tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 23, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pIR-CMV-SCN1A-Variant-1-IRES-mScarlet

    Plasmid

    #162278

    Purpose

    Eukaryotic expression of human SCN1A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.

    Depositor

    Al George

    Article

    DeKeyser et al J Biol Chem. 2021 Jan-Jun;296:100298. do

    Insert

    SCN1A Variant 1, stabilized (SCN1A Human)
    

    Tags

    IRES-mScarlet

    Expression

    Mammalian

    Mutation

    IVS intron inserted after bp2946. Silent codon c…

    Promoter

    CMV

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. EGFP-Cyclin A2

    Plasmid

    #215048

    Purpose

    Expresses EGFP-Cyclin A2 in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CCNA2 (CCNA2 Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. EGFP-Cyclin E1

    Plasmid

    #215053

    Purpose

    Expresses EGFP-Cyclin E1 in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CCNE1 (CCNE1 Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. mCherry-XRCC1

    Plasmid

    #215142

    Purpose

    Expresses mCherry-XRCC1 in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    XRCC1 (XRCC1 Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. HA-ERalpha

    Plasmid

    #215093

    Purpose

    Expresses HA-ERalpha in mammalian cells.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    ESR1 (ESR1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. EGFP-Cyclin F

    Plasmid

    #215054

    Purpose

    Expresses EGFP-Cyclin F in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CCNF (CCNF Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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