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p2R3a-3xVenusYFP-OcsT

Plasmid

#71271

Purpose

Entry clone containing three repeats of Venus. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway system

Depositor

Ari Pekka Mähönen

Publication

Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

Insert

3 times VenusYFP

Use

Gateway Cloning

Tags

octaline synthase terminator

Available since

Dec. 7, 2015

Availability

Academic Institutions and Nonprofits only

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AR-V7-pcw107-V5

Plasmid

#64636

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Publication

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

AR (transcript variant 1, splice isoform) (AR Human)

Use

Lentiviral

Tags

V5

Mutation

splice isoform *

Promoter

PGK

Available since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
Cx26-msfGFP

Plasmid

#69016

Purpose

Expresses rat Cx26 (Gjb2 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to monomerized superfolder Green Fluorescent Protein. Expression driven by CMV promoter.

Depositor

David Spray

Publication

Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

Insert

Cx26 (Gjb2 Rat)

Tags

V206K monomerized super folder GFP

Expression

Mammalian

Promoter

CMV

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO.1-sh-mSlug-4

Plasmid

#40648

Depositor

Bob Weinberg

Publication

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

sh-mSlug-4 (Snai2 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

hU6

Available since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pBabe Smad4J Rescue

Plasmid

#19153

Depositor

Joan Massague

Publication

Padua et al Cell. 2008 Apr 4. 133(1):66-77.

Insert

SMAD family member 4 (SMAD4 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

G to A at bp 1397 T to C at bp 1398

Available since

Sept. 3, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pPSU80

Plasmid

#173834

Purpose

Expresses 80 kD Penn State ladder protein

Depositor

Song Tan

Publication

Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

Insert

STRHSTPABPACIL1bRCC1

Expression

Bacterial

Promoter

T7

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pPSU40

Plasmid

#173831

Purpose

Expresses 40 kD Penn State ladder protein

Depositor

Song Tan

Publication

Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

Insert

HSTPABPACGST

Expression

Bacterial

Promoter

T7

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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p3E-csGBP

Plasmid

#108891

Purpose

Multisite gateway vector for 3' tagging with a conditionally stable nanobody against GFP

Depositor

Rob Parton

Publication

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

csGBP

Use

Gateway Cloning

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pF CAG luc IRES neo

Plasmid

#98294

Purpose

For constitutive expression of firefly luciferase from the CAG promoter together with an IRES-linked neo resistance gene

Depositor

Brett Stringer

Publication

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Inserts

Firefly luciferase
IRES
Neomycin resistance gene (NeoR)

Use

Lentiviral and Luciferase

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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p2R3a-Tq2CFP-OcsT

Plasmid

#71268

Purpose

Entry clone containing Turqoise2. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway system

Depositor

Ari Pekka Mähönen

Publication

Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

Insert

Tq2CFP

Use

Gateway Cloning

Tags

4xGly linker and octaline synthase terminator

Available since

Dec. 7, 2015

Availability

Academic Institutions and Nonprofits only

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mCherry-H2B-6

Plasmid

#55056

Purpose

Localization: Nucleus/Histones, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Publication

Kuipers et al J Cell Biol. 2011 Jan 10;192(1):29-41. d

Insert

H2B (H2BC11 Human)

Tags

mCherry

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
Pet22b-mEGFP

Plasmid

#166439

Purpose

Bacterial expression of 6x His tagged mEGFP

Depositor

Shuguo Sun

Publication

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP

Expression

Bacterial

Available since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pTHSSe_42

Plasmid

#109240

Purpose

pSC101 BbsI cloning vector

Depositor

Christopher Voigt

Publication

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

sfGFP

Expression

Bacterial

Promoter

PJ23105

Available since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pUBQ10-GW

Plasmid

#209370

Purpose

Gateway cloning compatible binary vector for agrobacterium mediated transformation. MCS upstream of gateway, driven by UBQ10 promoter

Depositor

Lucia Strader

Publication

Michniewicz et al BMC Res Notes. 2015 Mar 3;8:63. doi: 10.

Type

Empty backbone

Expression

Plant

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pET15b-His-3C-irisin

Plasmid

#122612

Purpose

Expresses in E. coli: human irisin with Nt histag and 3C protease site for cleavage

Depositor

Harold Erickson

Publication

Albrecht et al Sci Rep. 2015 Mar 9;5:8889. doi: 10.1038

Insert

irisin (ectodomain of FNDC5) (Fndc5 Human, Bovine, Rat, Mouse)

Tags

Nt: histag-3C protease site

Expression

Bacterial

Promoter

T7 polymerase

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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minCMV-1xmNG-dPCP

Plasmid

#231092

Purpose

Encodes a 1xmNeonGreen-dPCP under control of a weak promoter (minCMV).

Depositor

John Ngo

Publication

Kuffner et al Nat Methods. 2025 Sep 22. doi: 10.1038/s

Insert

1xmNG-dMCP

Expression

Mammalian

Available since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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Amber C1

Plasmid

#27797

Depositor

Steven Vogel

Publication

Koushik et al Biophys J. 2006 Dec 15. 91(12):L99-L101.

Insert

mAmber C1

Expression

Mammalian

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only

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pPM180

Plasmid

#64822

Purpose

GUS overexpression in planta

Depositor

Detlef Weigel

Publication

Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

Insert

GUS

Expression

Plant

Available since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pET28a-ClfB_N2N3-HIS-ybbr

Plasmid

#101717

Purpose

ClfB N2 and N3 domains with C-terminal ybbr tag for covalent immobilization

Depositor

Hermann Gaub

Publication

Milles et al Science. 2018 Mar 30;359(6383):1527-1533

Insert

ClfB_N2N3

Tags

H3V 3C, HIS, and ybbr

Expression

Bacterial

Promoter

T7

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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EGFP-PIP5K1A

Plasmid

#202720

Purpose

expresses fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Publication

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

EGFP:PIP5K1A (PIP5K1A Human)

Expression

Mammalian

Available since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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