We narrowed to 9,978 results for: Met;
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Plasmid#31757DepositorInsertPatronin (Patronin Fly)
TagsGFPExpressionInsectMutationContains only the coiled-coil domain: aa 535-1457PromoterMetallothioneinAvailable SinceSept. 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGL2 p15 (-756/+68) SBR2 Mut 3' box
Plasmid#15711DepositorInsertp15 promoter (CDKN2B Human)
UseLuciferaseExpressionMammalianMutationMut 3' box in SBR2. The 3' box contains…Available SinceSept. 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-human cGAS
Plasmid#186853PurposeDoxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transductionDepositorAvailable SinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmirGLO-3'UTR mouse Il13 5xBoxB
Plasmid#207128PurposeTethered luciferase reporter for mouse Il13 BoxBDepositorAvailable SinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pF712-pET28a-Hs-delta44-GCDH-NHis wt
Plasmid#85113Purposeexpression of recombinant human mature (delta44) GCDH wt with N-terminal 6xHis-tagDepositorInserthuman mature (delta44) GCDH (GCDH Human)
Tags6xHisExpressionBacterialMutationsilent SNP: G1041TPromoterT7Available SinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
Linked Free ClLIS1 (M)
Plasmid#182487PurposeYeast integrative plasmid for expressing fusion protein ERG20(F96W-N127W)-ClLIS1, with linker sequence AG4TGGA (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3)DepositorInsertFPPS(M)-LS
UseSynthetic Biology; Metabolic engineeringExpressionYeastPromoterGAL10Available SinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag4A-MMP8 (P78S)
Plasmid#29546DepositorInsertMetalloproteinase protein 8 (MMP8 Human)
TagsFLAGExpressionBacterial and MammalianMutationP78SAvailable SinceJuly 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET11a_3xFlag-nsp14 (SARS-CoV-2)
Plasmid#169159PurposeTo express SARS-CoV-2 nsp14 in E. coliDepositorInsert3xFlag-nsp5CS[VATLQ]-nsp14 (ORF1ab SARS-CoV-2)
Tags3xFlag and nsp5CS[VATLQ]: predicted nsp5 protease…ExpressionBacterialMutationCodon optimised for E. coliPromoterT7Available SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-Nr4a1 double gRNAs
Plasmid#162791PurposeMultiplex Guide RNAs to generate Nr4a1 knockout by CRISPRDepositorAvailable SinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046809
Plasmid#145685PurposeBacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferaseDepositorInsertSARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCI-neo:β2-365AAQA368 L349M
Plasmid#35610DepositorInsertnAChR beta2 L349M 365AAQA368 (Chrnb2 mouse strain 129, Mouse)
ExpressionMammalianMutationL349M and 365AAQA368PromoterCMVAvailable SinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCDF-Az
Plasmid#197101PurposeTo express the azidophenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into azidophenylalanineDepositorInsertTyrRS variant, amber suppressor tRNA, kanamycin resistance gene
ExpressionBacterialMutationThr32, Asn107, Pro158, Leu159, Gln162, Arg286 (Ty…Available SinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEXP5-NT-CuSeCat
Plasmid#42568DepositorInsertmutated calmodulin gene
TagsPolyhistidine (6×His) region, TEV recognition sit…ExpressionBacterialMutationchanged Phenylalanine 92 to Glutamine acid, Valin…PromoterT7Available SinceMarch 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-ITR-PytR(PyOtR)-mCherry
Plasmid#204870PurposeExpresses pyrrolysyl tRNA variant "PyOtR" and a wild-type mCherry reporter; can be packaged into AAVDepositorInsertM. mazei pyrrolysyl tRNA mutant "PyOtR"
UseAAVExpressionMammalianMutationU25C; A3G, A4C, A5G, C6G, G63C, T65G, T66CPromoterU6Available SinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pB80-hsKIF5B(1-560)Rigor-L-mVenus
Plasmid#193720PurposeExpression of mVenus tagged human kinesin-1 (KIF5B aa1-560) to anchor microtubulesDepositorInserthsKIF5B(1-560)Rigor-L-mVenus (KIF5B Synthetic, Human)
TagsmVenusExpressionMammalianMutationhsKIF5B(aa1-560), RIGOR: G234A; mVenus: Met1Del, …PromoterChicken beta-actinAvailable SinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
Mouse GFP-PRMT7 LDIG(69-72)AAAA
Plasmid#65044PurposeExpresses Catalytically inactive GFP-PRMT7 LDIG(69-72)AAAA mutantDepositorInsertPRMT7 (Prmt7 Mouse)
UseBacterial propagationTagsGFPExpressionMammalianMutationL69A D70A I71A G72APromoterCMVAvailable SinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLX307/PTPN11 T73I
Plasmid#140939Purposeexpresses PTPN11 T73IDepositorInsertPTPN11 protein tyrosine phosphatase non-receptor type 11 [ Homo sapiens (human) ] (PTPN11 Human)
TagsV5ExpressionMammalianMutationT73IPromoterEF1aAvailable SinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNUTN6His Y426F/Y517F hTFNG
Plasmid#70084PurposeExpresses N-His tagged nonglycosylated human serum transferrin unable to bind iron in the C-lobeDepositorInsertmutated human serum transferrin (TF Human)
TagsHexa His tag and N-terminal signal peptide, 4 aa …ExpressionMammalianMutationAsn413 Asp, Asn611Asp, Ty426Phe, Tyr517PhePromoterSV40Available SinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNUT N6His Y95F/Y188F hTFNG
Plasmid#69601PurposeExpresses N-His tagged nonglycosylated human serum transferrin unable to bind iron in the N-lobeDepositorInsertmutated human serum transferrin (TF Human)
TagsHexa His tag and N-terminal signal peptide, 4 aa …ExpressionMammalianMutationAsn413 Asp, Asn611Asp, Tyr95Phe, Tyr188PhePromoterSV40Available SinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-REX-GECO0.9
Plasmid#61249PurposeExpresses REX-GECO0.9 in neuronsDepositorInsertREX-GECO0.9
UseAAVExpressionMammalianMutationSubstitutions relative to R-GECO1: P60R, V61W, R6…PromoterhSynAvailable SinceMarch 27, 2015AvailabilityAcademic Institutions and Nonprofits only