Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 6,562 results for: nls

Clear keywords
Showing: 3541 - 3560 of 6562 results

  2. pSLQ2842 pPB: CAG-GID1-VPR-IRES-Zeo-WPRE PGK-GAI-tagBFP-SadCas9

    Plasmid
    #84244
    Purpose
    Expresses GA-inducible VPR-Sa dCas9
    Depositor
    Stanley Qi
    Publication
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    GAI-tagBFP-Sa dCas9
    GID1-VPR
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    GAI-tagBFP-Nucleoplasmin NLS, GID1, IRES-Zeocin, …
    Expression
    Mammalian
    Mutation
    D10A, N580A and Synonymous mutation in PYL1 to re…
    Promoter
    CAG and PGK
    Available since
    Nov. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    84244-plasmid-map-sequence-id-160887

    Enlarge

  3. SpCas9-YE1-BE4max

    Plasmid
    #198546
    Purpose
    Expresses human codon-optimized SpCas9-YE1-BE4max and blasticidin resistance: EFS promoter-SpCas9-YE1-BE4max-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-YE1-BE4max
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198546-plasmid-map-sequence-id-390295

    Enlarge

  4. SpCas9-NG-SsAPOBEC3B

    Plasmid
    #198536
    Purpose
    Expresses human codon-optimized SpCas9-NG-SsAPOBEC3B and blasticidin resistance: EFS promoter-SpCas9-NG-SsAPOBEC3B-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NG-SsAPOBEC3B
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198536-plasmid-map-sequence-id-388885

    Enlarge

  5. VanGlow-GL

    Plasmid
    #83342
    Purpose
    for Gateway cloning of promoter elements upstream of a GFP::Luciferase(nls) reporter, facilitating qualitative and quantitative analysis of expression in transgenic fly lines and S2 cells.
    Depositor
    Cheng-Yu Lee
    Publication
    Janssens et al Dev Cell. 2017 Feb 27;40(4):367-380.e7.
    Type
    Empty backbone
    Use
    Luciferase
    Tags
    GFP::Luciferase(nls)
    Expression
    Insect
    Available since
    Dec. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    83342-plasmid-map-sequence-id-158671

    Enlarge

  7. Anti-ProC [IPI-HPC-4] (Recombinant Antibody)

    Antibody
    #218108-rAb
    Purpose
    Anti-Protein C tag chimeric recombinant antibody with fused mouse variable and rabbit constant domains; binds to EDQVDPRLIDGK sequence.
    Depositor
    Institute for Protein Innovation (IPI)
    Publication
    Stearns et al J Biol Chem. 1988 Jan 15;263(2):826-32.
    Recommended applications
    Western Blot
    Reactivity
    Human
    Source species
    Rabbit
    Isotype
    IgG
    Trial size
    Not available to purchase
    Available since
    May 14, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    Western Blot image by Institute for Protein Innovation (IPI)

  8. pAAV-EF1a-fDIO-Cre (AAV1)

    Viral prep
    #121675-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-EF1a-fDIO-Cre (#121675). In addition to the viral particles, you will also receive purified pAAV-EF1a-fDIO-Cre plasmid DNA. EF1a-driven, Flp recombinase dependent expression of Cre recombinase. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Esteban Engel, Alexander Nectow
    Publication
    Schneeberger et al Cell. 2019 Jun 24. pii: S0092-8674(19)30
    Promoter
    EF1a
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only

  10. pUC57-ITR2-H1- sgCTG Bilbo -eCMV-CjSpD8A-3XFLAG-SV40 NLS-ITR2

    Plasmid
    #210749
    Purpose
    Coding for CjSp D8A alongside Bilbo sgRNA targeting CAG repeats
    Depositor
    Vincent Dion
    Publication
    Mangin et al bioRxiv 2023.10.24.563270
    Inserts
    CjSp D8A Cas9
    Bilbo sgCAG
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, PolyA signal
    Expression
    Mammalian
    Mutation
    D8A, N-terminal Cj Cas9, C-terminal Sp Cas9
    Promoter
    H1 and eCMV
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    210749-plasmid-map-sequence-id-417988

    Enlarge

  11. VPR-ps-dSpCas9

    Plasmid
    #102855
    Purpose
    A single-chain light-controllable dSpCas9 with pdDronpa1.2 domains
    Depositor
    Michael Lin
    Publication
    Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dSpCas9, pdDronpa1.2
    Use
    CRISPR and Synthetic Biology
    Tags
    NLS and VP64-p64-Rta transcription activator
    Expression
    Mammalian
    Promoter
    PGK
    Available since
    Nov. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    102855-plasmid-map-sequence-id-199006

    Enlarge

  12. 3xFLAG-dCas9/pMXs-IG

    Plasmid
    #51258
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Publication
    Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:
    Insert
    3xFLAG-dCas9
    Use
    CRISPR and Retroviral
    Tags
    3xFLAG tag and NLS (nuclear localization signal)
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    51258-plasmid-map-sequence-id-77869

    Enlarge

  13. pBridge-tyrosinase.σ2

    Plasmid
    #197415
    Purpose
    Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-2 σ2 fusion protein in yeast (yeast three-hybrid assays)
    Depositor
    Juan Bonifacino
    Publication
    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.
    Inserts
    tyrosinase cytosolic tail
    AP-2 σ2
    Tags
    GAL4-DNA binding domain fragment, HA tag, and nuc…
    Expression
    Yeast
    Mutation
    encodes R42G substitution, contains silent substi…
    Promoter
    ADH1 and MET25
    Available since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197415-plasmid-map-sequence-id-388893

    Enlarge

  15. EMMA toolkit

    Plasmid kit
    #1000000119
    Purpose
    Collection of Golden Gate vectors and standardized genetic parts for the construction mammalian expression plasmids.
    Depositor
    Yizhi Cai
    Application
    Cloning and Synthetic Biology
    Vector type
    Mammalian Expression
    Cloning type
    Golden Gate
    Available since
    March 1, 2018
    Availability
    Academic Institutions and Nonprofits only

  16. pBUN6I11

    Plasmid
    #50580
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-KRAB, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance
    Depositor
    Qi-Jun Chen
    Publication
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    dCas9-KRAB
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG, HA, and NLS
    Mutation
    dCas9 that is defective in DNA cleavage; the maiz…
    Promoter
    OsU3p and Ubi1p
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    50580-plasmid-map-sequence-id-75637

    Enlarge

  17. pSL1180-HR-PUbECFP

    Plasmid
    #47917
    Purpose
    Donor plasmid for homologous recombination that expresses ECFP consitutively using the Aedes aegypti polyubiquitin (PUb) promoter.
    Depositor
    Leslie Vosshall
    Insert
    ECFP
    Tags
    NLS
    Expression
    Insect
    Promoter
    Aedes aegypti polyubiquitin (PUb)
    Available since
    Sept. 26, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    12 Articles
    47917-plasmid-map-sequence-id-68604

    Enlarge

  20. pAAV_BiPVe3_dTomato_nlsdTomato (AAV1)

    Viral prep
    #213940-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV_BiPVe3_dTomato_nlsdTomato (#213940). In addition to the viral particles, you will also receive purified pAAV_BiPVe3_dTomato_nlsdTomato plasmid DNA. Bicistronic expression of dTomato and nuclear-targeted dTomato under the control of the PV+ basket cell-targeting enhancer E3. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Gordon Fishell
    Publication
    Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1
    Tags
    dTomato
    Available since
    Sept. 23, 2025
    Availability
    Academic Institutions and Nonprofits only
Showing: 3541 - 3560 of 6562 results