We narrowed to 5,820 results for: pcas

1. pCK340

   Plasmid

   #192639

   Purpose

   Expresses dSpG and MCP-SoxS(R93A/S101A) on p15A-CmR

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

   Inserts

   Sp.pCas9-dSpG
   BBa_J23107-MCP-SoxS(R93A, S101A)
   

   Use

   CRISPR and Synthetic Biology

   Mutation

   SoxS has R93A and S101A mutations and dSpG has se…

   Promoter

   BBa_J23107 and Sp.pCas9

   Available Since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMIA3-Olônne

   Plasmid

   #214447

   Purpose

   Expresses eSpCas9 and gRNA targeting Olônne-18 GSH

   Depositor

   Roger Foo

   Article

   Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

   Insert

   eSpCas9-P2A-mRuby2-P2A-tp53dn
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCC_01 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9-NLS-2A-Puro-WPRE

   Plasmid

   #139086

   Purpose

   Expresses human codon-optimized SpCas9 nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.

   Depositor

   Neville Sanjana

   Article

   Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

   Insert

   SpCas9
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pL-CRISPR.SFFV.PAC

   Plasmid

   #57829

   Purpose

   Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin resistance, SFFV Promoter driven

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Inserts

   SpCas9
   Sp sgRNA scaffold
   SFFV
   P2A-PAC
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Available Since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-EF1a-SPdCas9-EGFP-2A-Blast

   Plasmid

   #71215

   Purpose

   dCas9 fused to EGFP

   Depositor

   Yonglun Luo

   Article

   Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

   Insert

   dSpCas9-EGFP
   

   Use

   Lentiviral

   Tags

   dSpCas9-EGFP

   Expression

   Mammalian

   Available Since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. BPK2797

   Plasmid

   #72251

   Purpose

   Human expression plasmid for SpCas9-VRQR variant: CMV-T7-humanSpCas9-VRQR(D1135V, G1218R, R1335Q, T1337R)-NLS-3xFLAG

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9 VRQR(D1135V/G1218R/R1335Q/T1337R)-NLS-3xFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   D1135V, G1218R, R1335Q, and T1337R

   Promoter

   CMV

   Available Since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330-sgRNA_Ago2_1

   Plasmid

   #73529

   Purpose

   Expresses a human codon-optimized SpCas9 and sgRNA targeting Ago2

   Depositor

   Constance Ciaudo

   Article

   Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

   Insert

   AGO2
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Feb. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

8. pX330-sgRNA_Ago2_2

   Plasmid

   #73530

   Purpose

   Expresses a human codon-optimized SpCas9 and sgRNA targeting Ago2

   Depositor

   Constance Ciaudo

   Article

   Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

   Insert

   AGO2
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Feb. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

9. pX330-NQL005-SOX2-sgRNA

   Plasmid

   #175553

   Purpose

   For transient expression of spCas9-nuclease and a sgRNA targeting the mouse SOX2 locus.

   Depositor

   Elzo de Wit

   Article

   Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

   Insert

   spCas9-nuclease and sgRNA against mouse SOX2 STOP Codon
   

   Use

   Mouse Targeting

   Expression

   Mammalian

   Available Since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCCL-PGK-SPdCas9-BFP-EGFP

    Plasmid

    #66821

    Purpose

    dCas9 fused to BFP and EGFP

    Depositor

    Yonglun Luo

    Article

    Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

    Insert

    dSpCas9-BFP-EGFP
    

    Tags

    dSpCas9-BFP-EGFP

    Expression

    Mammalian

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCK669

    Plasmid

    #192638

    Purpose

    Expresses dxCas9-NG and MCP-SoxS(R93A/S101A) on p15A-CmR

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

    Inserts

    Sp.pCas9-dxCas9-NG
    BBa_J23107-MCP-SoxS(R93A, S101A)
    

    Use

    CRISPR and Synthetic Biology

    Mutation

    SoxS has R93A and S101A mutations and dxCas9-NG h…

    Promoter

    BBa_J23107 and Sp.pCas9

    Available Since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX330-NQL007-NANOG-sgRNA

    Plasmid

    #175555

    Purpose

    For transient expression of spCas9-nuclease and a sgRNA targeting the mouse NANOG locus.

    Depositor

    Elzo de Wit

    Article

    Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

    Insert

    spCas9-nuclease and sgRNA against mouse NANOG STOP Codon
    

    Use

    Mouse Targeting

    Expression

    Mammalian

    Available Since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pU6-(BsaI)_CBh-Cas9

    Plasmid

    #135010

    Purpose

    Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9

    Depositor

    Yosef Shaul

    Article

    Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio

    Insert

    humanized S. pyogenes Cas9
    

    Tags

    3xFLAG (N terminal on insert)

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. p-miR-Report + WT miR-181 BS in NLK 3UTR

    Plasmid

    #22790

    Depositor

    Xin Wang

    Article

    Ji et al Hepatology. 2009 Aug . 50(2):472-80.

    Insert

    Wild-type miR-181 Binding Site in Nemo-like kinase 3 Untranslated Region (NLK Human)
    

    Use

    Luciferase

    Available Since

    March 24, 2010

    Availability

    Academic Institutions and Nonprofits only

15. pUDR211

    Plasmid

    #113870

    Purpose

    expression of Cas9 programming sgRNA3 and sgRNA4 targetting HXT8 and HXT1 respectively

    Depositor

    Jack Pronk, Jean-Marc Daran

    Article

    Wijsman et al FEMS Yeast Res. 2019 Jan 1;19(1). pii: 5

    Insert

    sgRNA3-HXT8 / sgRNA4-HXT14
    

    Expression

    Yeast

    Available Since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUDR418

    Plasmid

    #113875

    Purpose

    expression of double Cas9 programming sgRNA11 targetting STL1

    Depositor

    Jack Pronk, Jean-Marc Daran

    Article

    Wijsman et al FEMS Yeast Res. 2019 Jan 1;19(1). pii: 5

    Insert

    doble sgRNA11-STL1
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAV10.F6.loxP

    Plasmid

    #63201

    Purpose

    pCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into chrVI: 97873-98803 (NotI or BciVI).

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Available Since

    Oct. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. p-miR-Report + MT miR-181 BS in NLK 3UTR

    Plasmid

    #22791

    Depositor

    Xin Wang

    Article

    Ji et al Hepatology. 2009 Aug . 50(2):472-80.

    Insert

    Mutant miR-181 Binding Site in Nemo-like kinase 3 Untranslated Region (NLK Human)
    

    Use

    Luciferase

    Available Since

    March 24, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pFB-Flag-MyoD(QQQ)-E12

    Plasmid

    #26003

    Depositor

    Jeffrey Dilworth

    Article

    Dilworth et al Proc Natl Acad Sci U S A. 2004 Aug 10. 1

    Insert

    MyoD(QQQ)-E12 Heterodimer Fusion (Myod1 Mouse)
    

    Use

    Baculovirus transfer vector

    Tags

    Flag

    Mutation

    Changed Lysine 99 to Glutamine; Lysine 102 to Glu…

    Available Since

    Sept. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. p2T-CMV-miniSdd3-BE4max-BlastR

    Plasmid

    #204849

    Purpose

    For cytosine base editing using miniSdd3 in mammalian cells

    Depositor

    Caixia Gao

    Article

    Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

    Insert

    miniSdd3-nSpCas9(D10A)-UGI
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    D10A in SpCas9

    Promoter

    CMV, SV40, EM7

    Available Since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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