Addgene

We narrowed to 878 results for: RK2

Showing: 681 - 720 of 878 results

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BAF-S4A-tet-puro

Plasmid

#199649

Purpose

Doxycycline-inducible BAF expression

Depositor

William Hahn

Article

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

BAF (BANF1 Human)

Use

Lentiviral

Tags

Expression

Mutation

S4A

Promoter

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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BAF-S4D-tet-puro

Plasmid

#199650

Purpose

Doxycycline-inducible BAF expression

Depositor

William Hahn

Article

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

BAF (BANF1 Human)

Use

Lentiviral

Tags

Expression

Mutation

S4D

Promoter

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-NSF/S647A

Plasmid

#74938

Purpose

Mammalian expression of human NSF mutant S647A (mutation in the D2 domain of the protein)

Depositor

Elisa Greggio

Article

Belluzzi et al Mol Neurodegener. 2016 Jan 13;11(1):1. d

Insert

NSF (NSF Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S647A (mutation in the D2 domain of the protein)

Promoter

CMV

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
sgYAP-R-1

Plasmid

#229445

Purpose

knockout of YAP1

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

sgYAP-R-1 (YAP1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6;bU6;EFS

Available since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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sgYAP-R-2

Plasmid

#229446

Purpose

knockout of YAP1

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

sgYAP-R-2 (YAP1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6;bU6;EFS

Available since

Jan. 10, 2025

Availability

Academic Institutions and Nonprofits only

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sgNF2

Plasmid

#229433

Purpose

knockout of NF2

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

sgNF2 (NF2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6;bU6;EFS

Available since

Jan. 10, 2025

Availability

Academic Institutions and Nonprofits only

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sgMARK3-R-2

Plasmid

#229438

Purpose

knockout of MARK3

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

sgMARK3-R-2 (MARK3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6;bU6;EFS

Available since

Jan. 10, 2025

Availability

Academic Institutions and Nonprofits only

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sgYAP-R-3

Plasmid

#229447

Purpose

knockout of YAP1

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

sgYAP-R-3 (YAP1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6;bU6;EFS

Available since

Jan. 10, 2025

Availability

Academic Institutions and Nonprofits only

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FLAG-MARK3-GK-CR

Plasmid

#229424

Purpose

Lentiviral or overpexression of cDNA

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

MARK3-GK-CR (MARK3 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Gatekeeper mutation M132G; silent mutation CRISPR…

Promoter

EFS

Available since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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sgTAZ-R-2

Plasmid

#229449

Purpose

knockout of WWTR1 (TAZ)

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

sgTAZ-R-2 (WWTR1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6;bU6;EFS

Available since

Dec. 18, 2024

Availability

Academic Institutions and Nonprofits only

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sgTAZ-R-1

Plasmid

#229448

Purpose

knockout of WWTR1 (TAZ)

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

sgTAZ-R-1 (WWTR1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6;bU6;EFS

Available since

Dec. 18, 2024

Availability

Academic Institutions and Nonprofits only

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sgCDK1-ROSA

Plasmid

#229443

Purpose

knockout of CDK1

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

sgCDK1-ROSA (CDK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6;bU6;EFS

Available since

Dec. 18, 2024

Availability

Academic Institutions and Nonprofits only

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sgMARK3-R-3

Plasmid

#229439

Purpose

knockout of MARK3

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

sgMARK3-R-3 (MARK3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6;bU6;EFS

Available since

Dec. 18, 2024

Availability

Academic Institutions and Nonprofits only

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sgMARK3-R-1

Plasmid

#229437

Purpose

knockout of MARK3

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

sgMARK3-R-1 (MARK3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6;bU6;EFS

Available since

Dec. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV5D HA-Δ37, 115-133, 204-217 PPM1H

Plasmid

#207344

Purpose

Mammalian expression of HA-Δ37, 115-133, 204-217 PPM1H

Depositor

Suzanne Pfeffer

Article

Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

PPM1H (PPM1H Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Δ37, Δ115-133, Δ204-217

Promoter

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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PGK EGFP-LC3B

Plasmid

#200427

Purpose

Mammalian expression of fluorescently-tagged marker for autophagic vesicles

Depositor

Erika Holzbaur

Article

Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

Insert

MAP1LC3B (MAP1LC3B Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

PGK

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-guide-puro mBltp1 (KIAA1109) - 1

Plasmid

#198489

Purpose

lentiviral stable expression of mBltp1 (KIAA1109) gRNA 1

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

mBltp1 (4932438A13Rik Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-guide-puro mBltp1 (KIAA1109) - 2

Plasmid

#198490

Purpose

lentiviral stable expression of mBltp1 (KIAA1109) gRNA 2

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

mBltp1 (4932438A13Rik Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-guide-puro mAtp6v1a-1

Plasmid

#198477

Purpose

lentiviral stable expression of mAtp6v1a gRNA 1

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

mouse ATPv1a (Atp6v1a Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-guide-puro mAtp6v1a-2

Plasmid

#198478

Purpose

lentiviral stable expression of mAtp6v1a gRNA 2

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

mouse ATPv1a (Atp6v1a Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-Flag-NSF/T646A

Plasmid

#74937

Purpose

Mammalian expression of human NSF mutant T646A (mutation in the D2 domain of the protein)

Depositor

Elisa Greggio

Article

Belluzzi et al Mol Neurodegener. 2016 Jan 13;11(1):1. d

Insert

NSF (NSF Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

T646A (mutation in the D2 domain of the protein)

Promoter

CMV

Available since

April 26, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3-Flag-NSF/T645A

Plasmid

#74936

Purpose

Mammalian expression of human NSF mutant T645A (mutation in the D2 domain of the protein)

Depositor

Elisa Greggio

Article

Belluzzi et al Mol Neurodegener. 2016 Jan 13;11(1):1. d

Insert

NSF (NSF Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

T645A (mutation in the D2 domain of the protein)

Promoter

CMV

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pSIN-PAmCherry-mSNCA-NE

Plasmid

#102364

Purpose

Lentiviral overexpression of PA-mCherry synuclein alpha fusion

Depositor

Shu Leong Ho

Article

Ho et al Autophagy. 2019 Apr 14:1-24. doi: 10.108

Inserts

PA-mCherry
synuclein alpha (Snca Mouse)

Use

Lentiviral

Tags

NE

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

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pSIN-hSNCA-NE

Plasmid

#102366

Purpose

Lentiviral overexpression of synuclein alpha

Depositor

Shu Leong Ho

Article

Ho et al Autophagy. 2019 Apr 14:1-24. doi: 10.108

Insert

synuclein alpha (SNCA Human)

Use

Lentiviral

Tags

NE

Expression

Mammalian

Mutation

A silent mutation (333bp downstream of ATG) was i…

Promoter

EF-1a

Available since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-ITGB1BP3

Plasmid

#23393

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ITGB1BP3 (NMRK2 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
shERK1a-mlpx-puro

Plasmid

#65228

Purpose

encodes a shRNA against ERK1

Depositor

Gerardo Ferbeyre

Article

Deschenes-Simard et al Genes Dev. 2013 Apr 15;27(8):900-15. doi

Insert

shRNA against ERK2 (MAPK1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
sgLATS1/2-2

Plasmid

#229432

Purpose

knockout of LATS1 and LATS2

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

sgLATS1/2-2 (LATS1, LATS2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6;bU6;EFS

Available since

Dec. 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
sgLATS1/2-1

Plasmid

#229431

Purpose

knockout of LATS1 and LATS2

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

sgLATS1/2-1 (LATS1, LATS2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6;bU6;EFS

Available since

Dec. 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
sgMST1/2-2

Plasmid

#229430

Purpose

knockout of MST1 and MST2

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

sgMST1/2-2 (MST1, STK3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6;bU6;EFS

Available since

Dec. 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
sgMST1/2-1

Plasmid

#229429

Purpose

knockout of MST1 and MST2

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

sgMST1/2-1 (MST1, STK3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6;bU6;EFS

Available since

Dec. 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
sgYAP-TAZ-3

Plasmid

#229453

Purpose

knockout of YAP1 and WWTR1 (TAZ)

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

sgYAP-TAZ-3 (YAP1, WWTR1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6;bU6;EFS

Available since

Dec. 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
His-SUMO-Rab10 Q68L

Plasmid

#236720

Purpose

Expresses Rab10 Q68L with His tag and SUMO cleavage sequence

Depositor

Suzanne Pfeffer

Article

Claire Y. et al bioRxiv, 2025

Insert

Rab10 Q68L (RAB10 Human)

Use

Tags

6xHis-SUMO

Expression

Bacterial

Mutation

Q68L

Promoter

T7

Available since

May 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
sgYAP-TAZ-2

Plasmid

#229452

Purpose

knockout of YAP1 and WWTR1 (TAZ)

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

sgYAP-TAZ-2 (YAP1, WWTR1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6;bU6;EFS

Available since

Jan. 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV5D HA-PPM1H_M flap

Plasmid

#236718

Purpose

Expresses PPM1H with PPM1M flap domain sequence

Depositor

Suzanne Pfeffer

Article

Claire Y. et al bioRxiv, 2025

Insert

PPM1H_M flap (PPM1H, PPM1M Human)

Use

Tags

HA

Expression

Mammalian

Mutation

PPM1H flap domain replaced with PPM1M flap domain

Promoter

CMV

Available since

May 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV5D HA-PPM1M_H flap

Plasmid

#236719

Purpose

Expresses PPM1M with PPM1H flap domain sequence

Depositor

Suzanne Pfeffer

Article

Claire Y. et al bioRxiv, 2025

Insert

PPM1M_H flap (PPM1M, PPM1H Human)

Use

Tags

HA

Expression

Mammalian

Mutation

PPM1M flap domain replaced with PPM1H flap domain

Promoter

CMV

Available since

May 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
T4 Lysozyme D20A WT*

Plasmid

#18241

Depositor

Brian Matthews

Insert

T4 Lysozyme (e Bacteriophage T4)

Use

Tags

Expression

Bacterial

Mutation

D20A WT*

Promoter

Available since

Aug. 26, 2008

Availability

Academic Institutions and Nonprofits only
T4 Lysozyme D20S WT*

Plasmid

#18242

Depositor

Brian Matthews

Insert

T4 Lysozyme (e Bacteriophage T4)

Use

Tags

Expression

Bacterial

Mutation

D20S WT*

Promoter

Available since

Aug. 26, 2008

Availability

Academic Institutions and Nonprofits only
T4 Lysozyme T26H WT*

Plasmid

#18251

Depositor

Brian Matthews

Insert

T4 Lysozyme (e Bacteriophage T4)

Use

Tags

Expression

Bacterial

Mutation

T26H WT*

Promoter

Available since

Aug. 26, 2008

Availability

Academic Institutions and Nonprofits only
pEMB52/Armadillo_34_373

Plasmid

#40543

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2

Use

Baculovirus

Tags

6xHis

Expression

Mutation

WT

Promoter

polH

Available since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pEMB52/Horse_34_374

Plasmid

#40544

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2

Use

Baculovirus

Tags

6xHis

Expression

Mutation

WT

Promoter

polH

Available since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge

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