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pCS2 Smad1 EVE GM

Plasmid

#27997

Depositor

Edward De Robertis

Article

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

Human Smad1 EVE Gsk3 mutant (SMAD1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

S463E S465E S210A T202A S191A S198A

Promoter

Available since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only
pCS2 hSmad1-MM

Plasmid

#22990

Depositor

Edward De Robertis

Article

Pera et al Genes Dev. 2003 Dec 15. 17(24):3023-8.

Insert

Smad1 (SMAD1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S214A, S206A, S195A, S187A

Promoter

Available since

July 21, 2010

Availability

Academic Institutions and Nonprofits only
pCS2 hSmad1-GM

Plasmid

#22992

Depositor

Edward De Robertis

Article

Fuentealba et al Cell. 2007 Nov 30. 131(5):980-93.

Insert

Smad1 (SMAD1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S210A ,T202A, S191A, S198A

Promoter

Available since

June 17, 2010

Availability

Academic Institutions and Nonprofits only
p3xFLAG CMV14 / BRAF-D594N

Plasmid

#131713

Purpose

Mammalian Expression of Flag tagged BRAF

Depositor

Jacques De Grève

Article

Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

Insert

BRAF (BRAF Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

D594N

Promoter

CMV

Available since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pSty11

Plasmid

#12401

Depositor

Titia de Lange

Article

de Lange et al EMBO J. 1992 Feb . 11(2):717-24.

Insert

Sty11

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 7, 2006

Availability

Academic Institutions and Nonprofits only
MSCV-JAK3(M511)_IRES_GFP

Plasmid

#206290

Purpose

Expression of mutant JAK3 (M511I) that is able to drive constitutive activation of the JAK/STAT pathway

Depositor

Charles de Bock

Article

Omari et al Heliyon. 2023 Nov 8;9(11):e22085. doi: 1

Insert

JAK3 (M511I)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Methionine 511 to Isoleucine

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2 xenopus sizzled

Plasmid

#24684

Depositor

Edward De Robertis

Article

Lee et al Cell. 2006 Jan 13. 124(1):147-59.

Insert

Sizzled (szl Frog)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 22, 2010

Availability

Academic Institutions and Nonprofits only
VPS13D^Halo

Plasmid

#174108

Purpose

The insert is a codon optimized DNA sequence encoding VPS13D with an internal Halo tag.

Depositor

Pietro De Camilli

Article

Guillen-Samander et al J Cell Biol. 2021 May 3;220(5). pii: 212

Insert

Codon optimized VPS13D

Use

Tags

Halo

Expression

Mammalian

Mutation

With an internal Halo tag in aminoacid position 1…

Promoter

CMV

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCD5-TS-sfGFP-Tetrabrachion-TEV-N2NL03

Plasmid

#158793

Purpose

Expresses influenza A virus tetrameric neuraminidase fused to a sfGFP

Depositor

Robert de Vries

Article

van der Woude et al Protein Sci. 2020 Jul 25. doi: 10.1002/p

Insert

Neuraminidase A/NL/109/03

Use

Tags

TwinStrep_sfGFP_tetrabrachion

Expression

Mammalian

Mutation

AA61-469

Promoter

CMV

Available since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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DsRed-Rab5-QL

Plasmid

#29688

Depositor

Edward De Robertis

Article

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

RAB5A

Use

Tags

DSRed2

Expression

Mammalian

Mutation

Q79L

Promoter

Available since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only
pEGFPC1-EPSIN 2

Plasmid

#22242

Depositor

Pietro De Camilli

Article

Rosenthal et al J Biol Chem. 1999 Nov 26. 274(48):33959-

Insert

Epsin 2 (EPN2 Human)

Use

Tags

GFP

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Dec. 3, 2009

Availability

Academic Institutions and Nonprofits only
pCS2 DN-GSK3-GFP

Plasmid

#29681

Depositor

Edward De Robertis

Article

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

glycogen synthase kinase 3

Use

Tags

GFP

Expression

Mammalian

Mutation

K85R and K86R

Promoter

Available since

July 7, 2011

Availability

Academic Institutions and Nonprofits only
p3xFLAG CMV14 / BRAF-D594A

Plasmid

#131717

Purpose

Mammalian Expression of Flag tagged BRAF

Depositor

Jacques De Grève

Article

Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

Insert

BRAF (BRAF Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

D594A

Promoter

CMV

Available since

Nov. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pLPC-NMYC-mTRF1

Plasmid

#64162

Purpose

Retroviral vector expressing mouse TRF1 with N-terminal Myc tag

Depositor

Titia de Lange

Article

Zimmermann et al Genes Dev. 2014 Nov 15;28(22):2477-91. d

Insert

mTRF1 (Terf1 Mouse)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 29, 2015

Availability

Academic Institutions and Nonprofits only
pLPC FLAG hRap1 delta BR delta CT

Plasmid

#13257

Depositor

Titia de Lange

Article

Li et al Mol Biol Cell. 2003 Dec . 14(12):5060-8.

Insert

Repressor Activator Protein 1 delta Br delta CT (TERF2IP Human)

Use

Retroviral

Tags

FLAG

Expression

Mutation

contains hRap1 aa2-40 + 96-270 +331-399 and the p…

Promoter

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pLPC FLAG hRap1 delta MC

Plasmid

#13254

Depositor

Titia de Lange

Article

Li et al Mol Biol Cell. 2003 Dec . 14(12):5060-8.

Insert

Repressor Activator Protein 1 delta MC (TERF2IP Human)

Use

Retroviral

Tags

FLAG

Expression

Mutation

contains hRap1 aa2-131 + 265-399 and the point mu…

Promoter

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3 Lunatic Fringe

Plasmid

#24962

Depositor

Edward De Robertis

Article

Pera et al Int J Dev Biol. 2005 . 49(7):781-96.

Insert

Lunatic Fringe (lfng Frog)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 18, 2010

Availability

Academic Institutions and Nonprofits only
pCS2 xDvl-RFP

Plasmid

#29683

Depositor

Edward De Robertis

Article

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

dishevelled (dvl2.L Frog)

Use

Tags

RFP

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2011

Availability

Academic Institutions and Nonprofits only
p3xFLAG CMV14 / BRAF-G469A

Plasmid

#131711

Purpose

Mammalian Expression of Flag tagged BRAF

Depositor

Jacques De Grève

Article

Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

Insert

BRAF (BRAF Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

G469A

Promoter

CMV

Available since

May 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEVA231-CRISPR

Plasmid

#138712

Purpose

Plasmid designed to clone a specific spacer into BsaI sites to generate a CRISPR Array

Depositor

Víctor de Lorenzo

Article

Velazquez et al ACS Synth Biol. 2019 Sep 20;8(9):2186-21

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLPC myc hPOT1 delta OB

Plasmid

#13241

Depositor

Titia de Lange

Article

Loayza et al Nature. 2003 Jun 26. 423(6943):1013-8.

Insert

Protection of Telomeres 1 delta OB (POT1 Human)

Use

Retroviral

Tags

Myc

Expression

Mutation

Promoter

Available since

Oct. 20, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a(+)-SUMO-A'-l4-G'

Plasmid

#209124

Purpose

Bacterial expression of CC-GEMS ligand SUMO-A'-l4-G'

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

SUMO-A'-l4-G'

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLeo-A-a2-GEMS(JAK-STAT)

Plasmid

#209110

Purpose

Transient mammalian expression of the CC functionalized GEMS receptor A-a2-GEMS (JAK-STAT)

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

A-a2-GEMS(JAK-STAT)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-SUMO-A'-l4-G'-IRES-EmGFP

Plasmid

#209127

Purpose

Lentiviral vector including gene for CC-GEMS ligand SUMO-A'-l4-G' and EmGFP

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

SUMO-A'-l4-G'-IRES-EmGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-SUMO-A'-l4-G-IRES-EmGFP

Plasmid

#209126

Purpose

Lentiviral vector including gene for CC-GEMS ligand SUMO-A'-l4-G and EmGFP

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

SUMO-A'-l4-G-IRES-EmGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Geph CR shRNA

Plasmid

#121068

Purpose

shRNA targeting the coding region of the gephyrin mRNA

Depositor

Angel de Blas

Article

Yu et al Mol Cell Neurosci. 2007 Dec;36(4):484-50

Insert

GPHN shRNA (Gphn Rat)

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2 xenopus sizzled HA

Plasmid

#24683

Depositor

Edward De Robertis

Article

Lee et al Cell. 2006 Jan 13. 124(1):147-59.

Insert

Sizzled (szl Frog)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

July 22, 2010

Availability

Academic Institutions and Nonprofits only
pCS2 xenopus chordin myc

Plasmid

#24682

Depositor

Edward De Robertis

Article

Piccolo et al Cell. 1996 Aug 23. 86(4):589-98.

Insert

Chordin

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
m2-26 mTRF2

Plasmid

#12300

Depositor

Titia de Lange

Article

Broccoli et al Nat Genet. 1997 Oct . 17(2):231-5.

Insert

mTRF2 (Terf2 )

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pBluescript SK +/- mTRF1

Plasmid

#12303

Depositor

Titia de Lange

Article

Broccoli et al Hum Mol Genet. 1997 Jan . 6(1):69-76.

Insert

mTRF1 (Terf1 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only
EYFP-NLS-actin-K68/284R

Plasmid

#60616

Purpose

Encodes YFP and NLS tagged beta-actin with sumoylation resistant mutations

Depositor

Primal de Lanerolle

Article

Hofmann et al J Cell Biol. 2009 Jul 27;186(2):193-200.

Insert

Beta-actin (ACTB Human)

Use

Tags

EYFP and NLS

Expression

Mammalian

Mutation

K68R, 284R

Promoter

Available since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only
RNF34

Plasmid

#119937

Purpose

RING finger protein 34 expression

Depositor

Angel de Blas

Article

Jin et al J Biol Chem. 2014 Oct 17;289(42):29420-3

Insert

RNF34 (Rnf34 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 6, 2019

Availability

Academic Institutions and Nonprofits only
pCS2 xenopus Frzb-1

Plasmid

#24694

Depositor

Edward De Robertis

Article

Pera et al Mech Dev. 2000 Sep . 96(2):183-95.

Insert

Frzb-1 (frzb-1 Frog)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 22, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3 xNoggin

Plasmid

#24960

Depositor

Edward De Robertis

Article

Pera et al Int J Dev Biol. 2005 . 49(7):781-96.

Insert

Noggin (nog.L Frog)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 18, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2 xenopus cerberus

Plasmid

#24679

Depositor

Edward De Robertis

Article

Bouwmeester et al Nature. 1996 Aug 15. 382(6592):595-601.

Insert

Cerberus (cer1 Frog)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
pNQL001-WAPL-AID[71-114]-eGFP-FRT-Neo/Kan-FRT targeting construct

Plasmid

#175549

Purpose

Targeting vector to introduce an AID-eGFP cassette at the mouse WAPL locus using Neomycin/Kanamycin selection. Auxin-inducible degron system.

Depositor

Elzo de Wit

Article

Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

Insert

WAPL (Wapl Mouse)

Use

Mouse Targeting

Tags

AID[71-114]-eGFP

Expression

Mammalian

Mutation

none

Promoter

Available since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pWZL Hygro-NMYC TRF2

Plasmid

#16348

Depositor

Titia de Lange

Article

Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

Insert

hTRF2 (TERF2 Human)

Use

Retroviral

Tags

MYC

Expression

Mammalian

Mutation

aa2-500

Promoter

Available since

May 14, 2008

Availability

Academic Institutions and Nonprofits only
pLPC MYC hTel2

Plasmid

#22802

Depositor

Titia de Lange

Article

Takai et al Cell. 2007 Dec 28. 131(7):1248-59.

Insert

Telomere Maintenance 2 (TELO2 Human)

Use

Retroviral

Tags

MYC

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
pX330-NQL005-SOX2-sgRNA

Plasmid

#175553

Purpose

For transient expression of spCas9-nuclease and a sgRNA targeting the mouse SOX2 locus.

Depositor

Elzo de Wit

Article

Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

Insert

spCas9-nuclease and sgRNA against mouse SOX2 STOP Codon

Use

Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2 mouse CV2 PC

Plasmid

#25776

Depositor

Edward De Robertis

Article

Coffinier et al Mech Dev. 2002 Dec . 119 Suppl 1():S179-

Insert

cross veinless 2 (Bmper Mouse)

Use

Tags

PC

Expression

Mammalian

Mutation

Promoter

Available since

July 2, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge

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