We narrowed to 1,321 results for: HRAS

1. pET22b - PA

   Plasmid

   #84863

   Purpose

   bacterial expression of Bacillus anthracis protective antigen

   Depositor

   Russell Vance

   Article

   Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

   Insert

   PA
   

   Use

   Tags

   pelB signal sequence

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pclbw-mitoCFP

   Plasmid

   #58426

   Purpose

   Mammalian expression of CFP targeted to the mitochondrial matrix

   Depositor

   David Chan

   Article

   Mishra et al Cell Metab. 2014 Apr 1;19(4):630-41. doi

   Insert

   mito-CFP
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   cmv-bactin

   Available Since

   Dec. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pclbw-mitoTagRFP

   Plasmid

   #58425

   Purpose

   Mammalian expression of tagRFP targeted to the mitochondrial matrix

   Depositor

   David Chan

   Article

   Mishra et al Cell Metab. 2014 Apr 1;19(4):630-41. doi

   Insert

   mito-tagRFP
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   cmv-bactin

   Available Since

   Dec. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-IL2sp-6His-Asprosin plasmid

   Plasmid

   #190683

   Purpose

   Expresses His-tagged asprosin in mammalian cells. An IL2 signal peptide at N-terminus allows secretion of asprosin from the transduced cells.

   Depositor

   Atul Chopra

   Article

   Mishra et al STAR Protoc. 2022 Dec 16;3(4):101762. do

   Insert

   IL2-6xHis-Asprosin
   

   Use

   Tags

   IL2 signal peptide, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. p414GPD_Hsp82viiB

   Plasmid

   #61709

   Purpose

   Yeast plasmid with Hsp82 heterodimer construct.

   Depositor

   Dan Bolon

   Article

   Mishra et al Mol Cell. 2014 Jan 23;53(2):344-50. doi:

   Insert

   Hsp82viiB (HSP82 Budding Yeast)
   

   Use

   Tags

   6xHis

   Expression

   Yeast

   Mutation

   Heteromeric coiled coil inserted after amino acid…

   Promoter

   GPD1

   Available Since

   Feb. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. PNMT

   Plasmid

   #64078

   Purpose

   Expression of human Phenylethanolamine N-methyltransferase

   Depositor

   Vern Schramm

   Article

   Stratton et al ACS Chem Biol. 2017 Feb 17;12(2):342-346

   Insert

   Phenylethanolamine N-methyltransferase (PNMT Synthetic, Human)
   

   Use

   Tags

   Histidine tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

7. BHMT

   Plasmid

   #64075

   Purpose

   Expression of betaine homocysteine methyltransferase

   Depositor

   Vern Schramm

   Article

   Poulin et al J Org Chem. 2015 May 15;80(10):5344-7. d

   Insert

   betaine-homocysteine methyltransferase (BHMT Synthetic, Human)
   

   Use

   Tags

   none

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. Hp MTAN

   Plasmid

   #69802

   Purpose

   Expression of H. pylori J99 methylthioadenosine nucleosidase

   Depositor

   Vern Schramm

   Article

   Wang et al Biochemistry. 2012 Sep 4;51(35):6892-4.

   Insert

   Methylthioadenosine nucleosidase
   

   Use

   Tags

   Histidine tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pENTR2b-mVenus

   Plasmid

   #154898

   Purpose

   mVenus entry vector

   Depositor

   Johanna Ivaska

   Article

   Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:

   Type

   Empty backbone

   Use

   Entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSM045

    Plasmid

    #217399

    Purpose

    KmR, pBTRcK broad host range vector with BTE1(C12-specific fatty acid thioesterase codon-optimised for C. necator) under Ptrc

    Depositor

    Brian Pfleger

    Article

    Mishra et al J Ind Microbiol Biotechnol. 2024 Feb 16:

    Insert

    BTE1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PTrc

    Available Since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSM046

    Plasmid

    #217400

    Purpose

    KmR, pBTRcK broad host range vector with BTE2(C12-specific fatty acid thioesterase codon-optimised for C. necator) under Ptrc

    Depositor

    Brian Pfleger

    Article

    Mishra et al J Ind Microbiol Biotechnol. 2024 Feb 16:

    Insert

    BTE2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PTrc

    Available Since

    June 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. P. falciparum DHPS

    Plasmid

    #64080

    Purpose

    Expression of P. falciparum dihydropteroate synthase

    Depositor

    Vern Schramm

    Article

    Stratton et al ACS Chem Biol. 2015 Oct 16;10(10):2182-6

    Insert

    dihydropteroate synthase
    

    Use

    Tags

    Histidine tag

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEF.DEST51-mVenus

    Plasmid

    #154899

    Purpose

    Expresses a fluorescent protein, mVenus

    Depositor

    Johanna Ivaska

    Article

    Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:

    Insert

    mVenus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α

    Available Since

    July 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. CMV-OMMjRCaMP-P2A-mito4xGCaMP6f

    Plasmid

    #127872

    Purpose

    Dual expression vector for mitochondrial matrix and outer membrane Ca2+ sensors

    Depositor

    Timothy Ryan

    Article

    Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

    Insert

    Outer membrane localized jRCaMP1b - P2A - mito4xGCaMP6f
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPT-TAR C1

    Plasmid

    #141246

    Purpose

    Transformation Associated Recombination (TAR) cloned Phaeodactylum tricornutum Mitochondrial Genome - Clone 1

    Depositor

    Bogumil Karas

    Article

    Cochrane et al Algal Research, 2020. 50, 102014

    Insert

    Mitochondrial Genome of Phaeodactylum tricornutum
    

    Use

    Synthetic Biology; Diatom expression

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Minor additional mutations described in manuscript

    Promoter

    Available Since

    July 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. Human PNP his-tagged

    Plasmid

    #64076

    Purpose

    Expression of human Purine Nucleoside Phosphorylase

    Depositor

    Vern Schramm

    Article

    Lewandowicz et al J Biol Chem. 2003 Aug 22;278(34):31465-8

    Insert

    Purine Nucleoside Phosphorylase (PNP Human)
    

    Use

    Tags

    Histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pScalps Luciferase Zsgreen

    Plasmid

    #184784

    Purpose

    Lentiviral vector with luciferase gene under SFFV promoter and Zsgreen under Cyclophilin promoter

    Depositor

    Ajit Chande

    Article

    Mishra et al Front Cell Infect Microbiol. 2021 Apr 23

    Insert

    Firefly Luciferase and Zsgreen
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. human PNP non-tagged

    Plasmid

    #64199

    Purpose

    Expression of native human PNP (non-tagged)

    Depositor

    Vern Schramm

    Article

    Murkin et al Biochemistry. 2007 May 1;46(17):5038-49.

    Insert

    Purine Nucleoside Phosphorylase (PNP Human)
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET-MAT2A

    Plasmid

    #164820

    Purpose

    Expresses MAT2A in E. coli BL21(DE3)

    Depositor

    Vern Schramm

    Article

    Firestone et al J Am Chem Soc. 2017 Oct 4;139(39):13754-

    Insert

    Methionine Adenosyltransferase 2A (MAT2A Human)
    

    Use

    Tags

    6x His Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. human MTAP

    Plasmid

    #64077

    Purpose

    Expression of human 5'-methylthioadenosine phosphorylase

    Depositor

    Vern Schramm

    Article

    Guan et al Biochemistry. 2011 Nov 29;50(47):10408-1

    Insert

    5'-methylthioadenosine phosphorylase (MTAP Synthetic, Human)
    

    Use

    Tags

    Histidine tag and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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