We narrowed to 14,231 results for: crispr grnas

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pNeoSagRNAH

Plasmid

#123266

Purpose

Expresses gRNA of Staphylococcus aureus Cas9 (SaCas9) in Leishmania

Depositor

Greg Matlashewski

Article

Zhang et al mSphere. 2019 Aug 21;4(4). pii: 4/4/e004

Type

Empty backbone

Use

CRISPR; Leishmania expression

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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PtPuc3_diaCas9_sgRNA

Plasmid

#109219

Purpose

Episome based vector with CRISPR/Cas9_sgRNA module for genome editing in Phaeodactylum tricornutum

Depositor

Per Winge

Article

Sharma et al Sci Rep. 2018 Sep 26;8(1):14401. doi: 10

Inserts

LHCF2 promoter
diaCas9
LHCF1 terminator
U6 promoter
sgRNA
U6 3' region

Use

CRISPR and Synthetic Biology

Promoter

LHCF1 terminator, LHCF2 promoter, U6 3' regi…

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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sgRNA1_NANOG

Plasmid

#64153

Purpose

Photoactivatable transcription system. Lentiviral expression of NANOG sgRNA1. Also contains a CMV-puro-t2A-mCherry expression cassette

Depositor

Moritoshi Sato

Article

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA1_NANOG (NANOG Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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sgRNA4_NANOG

Plasmid

#64156

Purpose

Photoactivatable transcription system. Lentiviral expression of NANOG sgRNA4. Also contains a CMV-puro-t2A-mCherry expression cassette

Depositor

Moritoshi Sato

Article

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA4_NANOG (NANOG Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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sgRNA3_NANOG

Plasmid

#64155

Purpose

Photoactivatable transcription system. Lentiviral expression of NANOG sgRNA3. Also contains a CMV-puro-t2A-mCherry expression cassette

Depositor

Moritoshi Sato

Article

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA3_NANOG (NANOG Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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sgRNA2_NANOG

Plasmid

#64154

Purpose

Photoactivatable transcription system. Lentiviral expression of NANOG sgRNA2. Also contains a CMV-puro-t2A-mCherry expression cassette

Depositor

Moritoshi Sato

Article

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA2_NANOG (NANOG Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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pAc-sgRNA-Cas9

Plasmid

#49330

Purpose

Expresses sgRNA and Cas9-Puro in Drosophila S2 cells

Depositor

Ji-Long Liu

Article

Bassett et al Biol Open. 2013 Dec 10. pii: bio.2013712

Inserts

Cas9
dU6-sgRNA

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Insect

Mutation

Human codon optimised

Promoter

Actin-5c and Drosophila U6

Available Since

Dec. 19, 2013

Availability

Academic Institutions and Nonprofits only

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AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-ITR (AAV-KPL)

Plasmid

#60224

Purpose

Expresses Cre recombinase and Renilla luciferase from the EFS promoter and three U6-driven sgRNAs targeting Kras, p53, and Lkb1. Contains KrasG12D HDR donor. AAV backbone.

Depositor

Feng Zhang

Article

Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

Inserts

sgRNA
Renilla luciferase
Cre recombinase
KrasG12D HDR donor (Kras Mouse)

Use

AAV, CRISPR, Cre/Lox, and Mouse Targeting

Tags

Cre-HA and Rluc-P2A

Expression

Mammalian

Promoter

EFS, No promoter, and U6

Available Since

Oct. 9, 2014

Availability

Academic Institutions and Nonprofits only

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IL1RN gRNA4

Plasmid

#113133

Purpose

Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)

Depositor

Dominik Niopek

Article

Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

Insert

IL1RN guideRNA 4 (SpCas9 scaffold), co-expressed GFP (transfection marker)

Use

AAV, Synthetic Biology, and TALEN

Expression

Mammalian

Available Since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pgRNA-bacteria

Plasmid

#44251

Purpose

Expression of customizable guide RNA (gRNA) for bacterial gene knockdown

Depositor

Stanley Qi

Article

Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

Insert

guide RNA (bacteria)

Use

CRISPR

Expression

Bacterial

Promoter

BBa_J23119 (SpeI)

Available Since

April 5, 2013

Availability

Academic Institutions and Nonprofits only

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pLH-spsgRNA2

Plasmid

#64114

Purpose

Vector for expression of Sp sgRNA2 in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Sp sgRNA2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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sgRNA AAVS1

Plasmid

#128119

Purpose

Co-expresses sgRNA AAVS1, SpyCas9 scaffold (F+E) and GFP (transfection marker)

Depositor

Dominik Niopek

Article

Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

Insert

sgRNA AAVS1 + GFP

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

RSV/U6

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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sgRNA EMX1

Plasmid

#128121

Purpose

Co-expresses sgRNA EMX1, SpyCas9 scaffold (F+E) and GFP (transfection marker)

Depositor

Dominik Niopek

Article

Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

Insert

sgRNA EMX1 + GFP

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

RSV/U6

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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sgRNA HBB

Plasmid

#128120

Purpose

Co-expresses sgRNA HBB, SpyCas9 scaffold (F+E) and GFP (transfection marker)

Depositor

Dominik Niopek

Article

Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

Insert

sgRNA HBB + GFP

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

RSV/U6

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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sgRNA RUNX

Plasmid

#128122

Purpose

Co-expresses sgRNA RUNX, SpyCas9 scaffold (F+E) and GFP (transfection marker)

Depositor

Dominik Niopek

Article

Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

Insert

sgRNA RUNX + GFP

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

RSV/U6

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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sgRNA HEK

Plasmid

#128123

Purpose

Co-expresses sgRNA HEK, SpyCas9 scaffold (F+E) and GFP (transfection marker)

Depositor

Dominik Niopek

Article

Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

Insert

sgRNA HEK + GFP

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

RSV/U6

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pUC57kan-T7-gRNA-U6 V2

Plasmid

#115520

Purpose

To construct multiple sgRNA expression vector for CRISPR

Depositor

Xingxu Huang

Insert

sgRNA scaffolf-U6 promoter

Use

CRISPR

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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gRNA-mCtnnb1

Plasmid

#84542

Purpose

mCtnnb1 gRNA expressing vector

Depositor

Bon-Kyoung Koo

Article

Andersson-Rolf et al Nat Methods. 2017 Jan 30. doi: 10.1038/n

Insert

mCtnnb1 gRNA

Use

CRISPR

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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EC2_1_dCas9_sgRNA

Plasmid

#163710

Purpose

Yeast low copy plasmid with dCas9 and sgRNA expression cassette

Depositor

Yvonne Nygård

Article

Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

Insert

dCas9

Expression

Yeast

Mutation

Point mutations D10A and H840A

Promoter

ScTEF1

Available Since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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EC2_7_dCas9_HC_sgRNA

Plasmid

#163711

Purpose

High copy plasmid with dCas9 and sgRNA expression cassettes

Depositor

Yvonne Nygård

Article

Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

Insert

dCas9

Expression

Yeast

Mutation

Point mutations D10A and H840A

Promoter

ScTEF1

Available Since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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