We narrowed to 15,954 results for: epo

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pEPOR1CB0022

Plasmid

#117537

Purpose

Level1 Golden Gate Cassette: estended stem sgRNA cassette for SpCas9 targeting NbPDS gene in N. benthamiana

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

sgRNA (Sp Cas9) - extended stem

Expression

Plant

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0012

Plasmid

#117533

Purpose

Level0 Golden Gate part: CDS, eSaCas9 (no stop codon)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

eSaCas9 (no stop codon)

Use

CRISPR

Mutation

R499A,Q500K,R654A,G655R

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0019

Plasmid

#117529

Purpose

Level0 Golden Gate part: CDS, Sp-eCas9 1.1 (no stop codon)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

Sp-eCas9 1.1 (no stop codon)

Use

CRISPR

Mutation

K848A, K1003A, R1060A

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0018

Plasmid

#117528

Purpose

Level0 Golden Gate part: CDS, Sp-eCas9 1.1

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

Sp-eCas9 1.1 (with stop codon)

Use

CRISPR

Mutation

K848A, K1003A, R1060A

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0017

Plasmid

#117527

Purpose

Level0 Golden Gate part: CDS, Sp-eCas9 1.0 (no stop codon)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

Sp-eCas9 1.0 (no stop codon)

Use

CRISPR

Mutation

K810A, K1003A, R1060A

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0015

Plasmid

#117525

Purpose

Level0 Golden Gate part: CDS, SpCas9-KA (no stop codon)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SpCas9-KA (no stop codon)

Use

CRISPR

Mutation

K855A

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0014

Plasmid

#117524

Purpose

Level0 Golden Gate part: CDS, SpCas9-KA

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SpCas9-KA (with stop codon)

Use

CRISPR

Mutation

K855A

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0001

Plasmid

#117523

Purpose

Level0 Golden Gate part: CDS, SpCas9-DE (no stop codon)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SpCas9-DE (no stop codon)

Use

CRISPR

Mutation

D1135E

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0009

Plasmid

#117522

Purpose

Level0 Golden Gate part: CDS, SpCas9-p (no stop codon)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SpCas9-p (no stop codon)

Use

CRISPR

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR2KN0050

Plasmid

#117617

Purpose

Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.0(pEPOR1CB0010) and sgRNA_AtMPK19 {AT3G14720}(pEPOR1CB0081)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

[35S:Sp-eCas9 1.0(pEPOR1CB0010) ] +[AtU6-26:sgRNA]

Expression

Plant

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR2KN0049

Plasmid

#117616

Purpose

Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_AtMPK19 {AT3G14720}(pEPOR1CB0081)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

[35S:SpCas9-h(pICSL11023) ] +[AtU6-26:sgRNA]

Expression

Plant

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR2KN0051

Plasmid

#117618

Purpose

Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.1(pEPOR1CB0011) and sgRNA_AtMPK19 {AT3G14720}(pEPOR1CB0081)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

[35S:Sp-eCas9 1.1(pEPOR1CB0011) ] +[AtU6-26:sgRNA]

Expression

Plant

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0008

Plasmid

#117535

Purpose

Level0 Golden Gate part: CDS, AsCas12a

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

AsCas12a (with stop codon)

Use

CRISPR

Tags

3xHA and NLS

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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SepOTSι

Plasmid

#68290

Purpose

Phosphoserine orthogonal translation system

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

SepRS9/EFSep21, 2xtRNA-Sep-A37

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

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SepOTSη

Plasmid

#68288

Purpose

Phosphoserine orthogonal translation system

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

SepRS9/EF-Sep21, 2xtRNA-Sep

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

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SepOTSζ

Plasmid

#68287

Purpose

Phosphoserine orthogonal translation system

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

SepRS/EF-Sep, 2xtRNA-Sep

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

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SepOTSδ

Plasmid

#68285

Purpose

Phosphoserine orthogonal translation system

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

SepRS/EF-Sep, 1xtRNA-Sep-A37

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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Cre Reporter

Plasmid

#62732

Purpose

Lentiviral Cre recombinase reporter; EF1a-loxP-DsRedExpress2-loxP-EGFP. Cre excises the loxP-flanked RFP-STOP cassette and switches fluorescence from red (RFP) to green (GFP).

Depositor

Niels Geijsen

Article

D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

Insert

LoxP-DsRED-STOP-LoxP-eGFP

Use

Cre/Lox and Lentiviral

Tags

LoxP sites

Expression

Mammalian

Promoter

EF1-Alpha

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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p21-Reporter

Plasmid

#52432

Purpose

DNA damage inducible Red Fluorescent Protein for mammalian expression

Depositor

Joel Voldman

Article

Fendyur et al Anal Chem. 2014 Aug 5;86(15):7598-605. d

Insert

p21-p53 Tandem Response Element Promoter

Tags

TurboRFP

Expression

Mammalian

Promoter

13X repeated p21-p53 response element

Available Since

Oct. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pL-SFFV.Reporter.RFP657.PAC

Plasmid

#61395

Purpose

Lentiviral vector for RFP657 NHEJ reporter system expression. Allows insertion of target sites between the SFFV promoter and RFP657 for detection of fluorescence knockdown. Puromycin resistance.

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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