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Plasmid#178095PurposeControl vector for coselection for PE3b in human cells. Tandem expression of ATP1A1 G3 and EGFP Nick sgRNAs from two independent U6 promoters.DepositorInsertATP1A1 G3 + EGFP nick sgRNAs
UseCRISPR; Prime editingTagsExpressionMammalianMutationPromoterTandem U6 promotersAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(-10TC)_Psyn-sgRNAftsI
Plasmid#149591Purposeall-in-all CRISPRi vector for targeting B. burgdorferi ftsIDepositorInsertdCas9, lacI, sgRNAftsI
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30(-10TC), PsynAvailable sinceNov. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSC39
Plasmid#104813PurposeCRISPR/Cas9 2xplex gRNA targeting Medtr2g101180 (Cop-like). Also expresses Cas9 from Gmubi promoter.DepositorInsertMedtr2g101180
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSC32
Plasmid#104806PurposeCRISPR/Cas9 2xplex gRNA targeting Medtr2g101090 (Pen3). Also expresses Cas9 from AtUBQ10 promoter.DepositorInsertMedtr2g101090
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSC31
Plasmid#104805PurposeCRISPR/Cas9 2xplex gRNA targeting Medtr2g101090 (Pen3). Also expresses Cas9 from Gmubi promoter.DepositorInsertMedtr2g101090
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSC24
Plasmid#104798PurposeCRISPR/Cas9 2xplex gRNA targeting Medtr2g101120, Medtr2g101130 (Acre2). Also expresses Cas9 from rolD promoter from AtUBQ10 promoterDepositorInsertMedtr2g101120, Medtr2g101130
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSC14
Plasmid#104788PurposeCRISPR/Cas9 2xplex gRNA targeting Medtr2g044580 (ERDJ2). Also expresses Cas9 from AtUBQ10 promoterDepositorInsertMedtr2g044580
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSC15
Plasmid#104789PurposeCRISPR/Cas9 2xplex gRNA targeting Medtr2g044580 (ERDJ2). Also expresses Cas9 from rolD promoterDepositorInsertMedtr2g044580
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSC13
Plasmid#104787PurposeCRISPR/Cas9 2xplex gRNA targeting Medtr2g044580 (ERDJ2). Also expresses Cas9 from Gmubi promoterDepositorInsertMedtr2g044580
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pKDsgRNA-torZ
Plasmid#89956PurposeContains arabinose-induced lambda Red and a tet-inducible gRNA targeting torZ.DepositorInserttorZ gRNA
UseTagsExpressionBacterialMutationPromoterpTetAvailable sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
TU#1805_CRISPR_unc-73_exon2
Plasmid#82359Purposeto create unc-73B null alleleDepositorInsertCas9 and sgRNA against unc-73 exon2 (unc-73 Nematode)
UseCRISPRTagsExpressionWormMutationPromoterU6Available sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLKO1-puro-U6-sgRNA-PLXNB2
Plasmid#69240PurposeU6 driven SpCas9 sgRNA expression for PLXNB2 siteDepositorInsertPLXNB2 sgRNA (PLXNB2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
MS2-adRNA (5'GAC)
Plasmid#170127PurposeLentiviral vector carrying 2 copies of the MS2 adRNA targeting a GAC at the 5' end of the ADAR2 deaminase domain in plasmids #170124 and 170125DepositorInsertMS2-ADAR2(5'GAC)-MS2
UseLentiviralTagsExpressionMammalianMutationPromoterHuman U6 and mouse U6Available sinceAvailabilityAcademic Institutions and Nonprofits only -
ZO-1 shRNA 3253
Plasmid#37206DepositorInsertdog ZO-1 shRNA (TJP1 canine)
UseRNAiTagsExpressionMammalianMutationPromoterAvailable sinceAug. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCRA03
Plasmid#103141PurposeTriple gRNA expression separated with 28nt Csy4 motifDepositorInsertgRNAs
UseTagsExpressionYeastMutationPromoterAvailable sinceJan. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX330.LoxP
Plasmid#66586PurposesgRNA for LSLDepositorInsertLoxP sgRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pWT029g
Plasmid#96860Purposeguanine-agRNA (GFP activation, 18 nt blocker with bulges)DepositorInsertguanine-agRNA (GFP activation, 18 nt blocker with bulges)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWT029h
Plasmid#96861Purpose(d)guanine-agRNA (GFP activation,18 nt blocker with bulges)DepositorInsert(d)guanine-agRNA (GFP activation,18 nt blocker with bulges)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only