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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,403 results for: hras

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Showing: 341 - 360 of 1403 results

  2. pJYDNp

    Plasmid
    #162451
    Purpose
    Positive control plasmid carrying the eUnaG2 bilirubin dependent fluorescent protein fused to the C-terminus of Aga2p protein. This plasmid serves as an expression control.
    Depositor
    Gideon Schreiber
    Article
    Zahradník et al bioRxiv Preprint 2020
    Insert
    Aga2p-eUnaG2
    Use
    Shuttle vector bacteria/yeast
    Tags
    HA and myc
    Promoter
    GAL1
    Available Since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    162451-plasmid-map-sequence-id-321446

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  3. pJYDNg

    Plasmid
    #162452
    Purpose
    Yeast display plasmid intended for expression of N-terminal fusions with Aga2p yeast agglutinin. The plasmid is carrying the eUnaG2 fluorescent protein and long 2G linker.
    Depositor
    Gideon Schreiber
    Article
    Zahradník et al bioRxiv Preprint 2020
    Type
    Empty backbone
    Use
    Shuttle vector bacteria/yeast
    Tags
    Ha and myc and eUnaG2 fluorescent protein
    Available Since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    162452-plasmid-map-sequence-id-321450

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  4. pJYDC3

    Plasmid
    #162460
    Purpose
    Yeast display plasmid for expression of C-terminal fusions with Aga2p yeast agglutinin. The reporter DnbALFA was fused to the N-terminus of Aga2p.
    Depositor
    Gideon Schreiber
    Article
    Zahradník et al bioRxiv Preprint 2020
    Type
    Empty backbone
    Use
    Shuttle vector bacteria/yeast
    Tags
    DnbALFA designed ALFA nanobody, HA tag, HDEL sequ…
    Available Since
    April 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    162460-plasmid-map-sequence-id-330057

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  5. pJYDC2

    Plasmid
    #162459
    Purpose
    Yeast display plasmid for expression of C-terminal fusions. Modification of pJYDC1 vector with ALFA tag in MCS.
    Depositor
    Gideon Schreiber
    Article
    Zahradník et al bioRxiv Preprint 2020
    Type
    Empty backbone
    Use
    Shuttle vector bacteria/yeast
    Tags
    ALFA-tag, HA tag, HDEL sequence, and eUnaG2 fluor…
    Available Since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    162459-plasmid-map-sequence-id-321477

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  7. pJYDC1

    Plasmid
    #162458
    Purpose
    Yeast display plasmid for expression of C-terminal fusions with Aga2p yeast agglutinin. The reporter, eUnaG2 bilirubin dependent green-yellow fluorescent protein, is fused to the Nterminus
    Depositor
    Gideon Schreiber
    Article
    Zahradník et al bioRxiv Preprint 2020
    Type
    Empty backbone
    Use
    Shuttle vector bacteria/yeast
    Tags
    HA tag, HDEL sequence, eUnaG2 fluorescent protein…
    Available Since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    162458-plasmid-map-sequence-id-321474

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  8. pJYDN

    Plasmid
    #162450
    Purpose
    Yeast display plasmid intended for expression of N-terminal fusions with Aga2p yeast agglutinin and eUnaG2 bilirubin dependent green-yellow fluorescent protein for detection of expression.
    Depositor
    Gideon Schreiber
    Article
    Zahradník et al bioRxiv Preprint 2020
    Type
    Empty backbone
    Use
    Shuttle vector bacteria/yeast
    Tags
    Aga2p, HA, myc tags, and eUnaG2 fluorescent prote…
    Expression
    Yeast
    Available Since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    162450-plasmid-map-sequence-id-321442

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  9. pJYDN2

    Plasmid
    #162454
    Purpose
    Yeast display plasmid intended for co-cultivation labeling by using designed ALFA-tag binding nanobody (DnbALFA) and expression of N-terminal fusions with Aga2p yeast agglutinin.
    Depositor
    Gideon Schreiber
    Article
    Zahradník et al bioRxiv Preprint 2020
    Type
    Empty backbone
    Use
    Shuttle vector bacteria/yeast
    Tags
    DnbALFA nanobody and Ha and myc
    Available Since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    162454-plasmid-map-sequence-id-321461

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  10. pJYDN2p

    Plasmid
    #162455
    Purpose
    Positive control plasmid for co-cultivation labeling by using designed ALFA-tag binding nanobody (DnbALFA).
    Depositor
    Gideon Schreiber
    Article
    Zahradník et al bioRxiv Preprint 2020
    Insert
    Aga2p-DnbALFA (AGA2 )
    Use
    Shuttle vector bacteria/yeast
    Tags
    DnbALFA nanobody and HA and myc
    Promoter
    GAL1
    Available Since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    162455-plasmid-map-sequence-id-321462

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  11. pJYDN3

    Plasmid
    #162456
    Purpose
    Yeast display platform plasmid N3 which allows for simultaneous expression of the protein of interest at the N-terminus of Aga2p yeast agglutinin and eUnaG2 or nbALFA detection.
    Depositor
    Gideon Schreiber
    Article
    Zahradník et al bioRxiv Preprint 2020
    Type
    Empty backbone
    Use
    Shuttle vector bacteria/yeast
    Tags
    ALFA tag, HA, myc tags, and eUnaG2 fluorescent pr…
    Available Since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    162456-plasmid-map-sequence-id-321464

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  12. pJYDNgp

    Plasmid
    #162453
    Purpose
    Positive control plasmid for pJYDNg carrying the eUnaG2 bilirubin dependent fluorescent protein fused to the C-terminus of Aga2p protein. This plasmid contains a long 2G linker.
    Depositor
    Gideon Schreiber
    Article
    Zahradník et al bioRxiv Preprint 2020
    Insert
    Aga2p-2Glinker-eUnaG2 (AGA2 )
    Use
    Shuttle vector bacteria/yeast
    Tags
    HA nad myc and eUnaG2 fluorescent protein
    Promoter
    GAL1
    Available Since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    162453-plasmid-map-sequence-id-321460

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  13. pJYDN3p

    Plasmid
    #162457
    Purpose
    Positive control plasmid for pJYDN3n plasmid expression attempts. It contains both eUnaG2 bilirubin dependent fluorescent protein and ALFA-tag for DnbALFA based detection on the cell surface.
    Depositor
    Gideon Schreiber
    Article
    Zahradník et al bioRxiv Preprint 2020
    Insert
    Aga2p-eUnaG2 (AGA2 )
    Use
    Shuttle vector bacteria/yeast
    Tags
    ALFA tag, HA and myc tags, and eUnaG2 fluorescent…
    Promoter
    GAL1
    Available Since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    162457-plasmid-map-sequence-id-321472

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  14. Anti-GFP [N86/8R] (Recombinant Antibody)

    Antibody
    #184171-rAb
    Purpose
    Anti-GFP (Aequorea victoria) recombinant mouse monoclonal antibody. Cross-reacts with Cyan and Yellow fluorescent protein (CFP and YFP, respectively).
    Depositor
    James Trimmer
    Article
    Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1
    Recommended Applications
    Western Blot
    Reactivity
    Synthetic
    Source Species
    Mouse
    Isotype
    IgG2a
    Trial Size
    Available to purchase
    Available Since
    Oct. 14, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    Western Blot image by Addgene

  18. pAAV-EF1a-iFlpV (AAV1)

    Viral Prep
    #140137-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-EF1a-iFlpV (#140137). In addition to the viral particles, you will also receive purified pAAV-EF1a-iFlpV plasmid DNA. EF1a-driven, light-inducible iFlpV recombinase for spatiotemporally precise optogenomic modifications. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Hongkui Zeng
    Article
    Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s
    Promoter
    EF1a
    Available Since
    Oct. 28, 2021
    Availability
    Academic Institutions and Nonprofits only

  19. pAAV-EF1a-iDreV (AAV1)

    Viral Prep
    #140136-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-EF1a-iDreV (#140136). In addition to the viral particles, you will also receive purified pAAV-EF1a-iDreV plasmid DNA. EF1a-driven, light-inducible iDreV recombinase for spatiotemporally precise optogenomic modifications. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Hongkui Zeng
    Article
    Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s
    Promoter
    EF1a
    Available Since
    Oct. 28, 2021
    Availability
    Academic Institutions and Nonprofits only

  20. pAAV-EF1a-iCreV (AAV1)

    Viral Prep
    #140135-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-EF1a-iCreV (#140135). In addition to the viral particles, you will also receive purified pAAV-EF1a-iCreV plasmid DNA. EF1a-driven, light-inducible iCreV recombinase for spatiotemporally precise optogenomic modifications. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Hongkui Zeng
    Article
    Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s
    Promoter
    EF1a
    Available Since
    Sept. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
Showing: 341 - 360 of 1403 results