We narrowed to 5,806 results for: pcas

1. pCAG-Voltron-ST

   Plasmid

   #119034

   Purpose

   Express soma-localized Voltron in mammalian cells

   Depositor

   Eric Schreiter

   Article

   Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

   Insert

   Voltron-ST
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-T7pol

   Plasmid

   #59926

   Purpose

   Expression vector for T7 RNA polymerase gene

   Depositor

   Ian Wickersham

   Insert

   T7 RNA polymerase
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. T7opt in pCAGGS

   Plasmid

   #65974

   Purpose

   Human codon-optimized T7 polymerase

   Depositor

   Benhur Lee

   Article

   Yun et al J Virol. 2015 Jan 15;89(2):1242-53. doi:

   Insert

   T7-opt
   

   Expression

   Mammalian

   Available Since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-Flpo

   Plasmid

   #60662

   Purpose

   Expresses pCAG-Flpo; codon-optimized for mammalian expression

   Depositor

   Massimo Scanziani

   Article

   Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

   Insert

   Flpo
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSpCas9_T2A_iRFP670 sgBFP

   Plasmid

   #192478

   Purpose

   All-in-one plasmid for cutting BFP region in DSB Spectrum V2 or V3

   Depositor

   Michael Yaffe

   Article

   van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

   Insert

   SpCas9
   

   Tags

   T2A iRFP670

   Expression

   Mammalian

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. VPR-ps-dSpCas9

   Plasmid

   #102855

   Purpose

   A single-chain light-controllable dSpCas9 with pdDronpa1.2 domains

   Depositor

   Michael Lin

   Article

   Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021

   Insert

   dSpCas9, pdDronpa1.2
   

   Use

   CRISPR and Synthetic Biology

   Tags

   NLS and VP64-p64-Rta transcription activator

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Nov. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG/hArf4(WT)-HA

   Plasmid

   #79403

   Purpose

   Expresses C-terminally HA-tagged Arf4(WT) in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Article

   Nakai et al Mol Biol Cell. 2013 Aug;24(16):2570-81.

   Insert

   Arf4 (ARF4 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

8. pSpCas9_T2A_iRFP670 sgAAVS1

   Plasmid

   #192477

   Purpose

   All-in-one plasmid for cutting non-targeting locus in DSB Spectrum V1, V2, or V3 cells as a control

   Depositor

   Michael Yaffe

   Article

   van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

   Insert

   SpCas9
   

   Tags

   T2A iRFP670

   Expression

   Mammalian

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-Eco

   Plasmid

   #35617

   Depositor

   Patrick Salmon, Arthur Nienhuis

   Insert

   Ecotropic MLV envelope
   

   Expression

   Mammalian

   Available Since

   July 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCas9-CtIP

    Plasmid

    #109403

    Purpose

    Expresses Cas9 fusion to human CtIP protein

    Depositor

    Jean-Paul Concordet

    Article

    Charpentier et al Nat Commun. 2018 Mar 19;9(1):1133. doi:

    Insert

    CtIP (RBBP8 Human)
    

    Mutation

    full length CtIP

    Available Since

    June 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-CreERT2

    Plasmid

    #14797

    Purpose

    Mammalian expression of conditionally active form of Cre recombinase (not as tightly regulated as pCAG-ERT2CreERT2)

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

    Insert

    Cre-ERT2 fusion protein
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 20, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCas9-CAT

    Plasmid

    #80323

    Purpose

    Encodes Cas9 and chloramphenicol acetyltransferase (CAT)

    Depositor

    Sebastian Lourido

    Article

    Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

    Inserts

    Cas9
    Chloramphenicol acetyltransferase
    

    Use

    CRISPR

    Tags

    3X FLAG and NLS

    Promoter

    TgTUB1

    Available Since

    Sept. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCA24N-ligase

    Plasmid

    #87741

    Purpose

    IPTG-inducible expression of T4 DNA ligase for protein purification

    Depositor

    Wayne Patrick

    Article

    Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

    Insert

    T4 DNA ligase (30 )
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T5-lac

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-VSVG

    Plasmid

    #64084

    Purpose

    Expression vector for Vesicular Stomatitis Virus Indiana strain glycoprotein gene

    Depositor

    Ian Wickersham

    Insert

    VSV glycoprotein
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAFNF-GFP

    Plasmid

    #13772

    Purpose

    Flp recombinase-dependent expression of GFP, i.e. GFP is only expressed in the presence of Flp due to a transcriptional stop in between the FRT sites.

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

    Insert

    EGFP
    

    Use

    Flp/frt

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 20, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-mTagBFP2

    Plasmid

    #122373

    Purpose

    Expresses mTagBFP2 in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    mTagBFP2
    

    Expression

    Mammalian

    Available Since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-FlpO

    Plasmid

    #89574

    Purpose

    Expresses FlpO under pCAG

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    FlpO
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Available Since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-TetON-3G

    Plasmid

    #96963

    Purpose

    Mammalian expression of Clontech rtTA driven by human beta-actin promoter

    Depositor

    Elena Cattaneo

    Article

    Faedo et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Aug. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET-21a_3xNLS_SpCas9_protein_expression

    Plasmid

    #114365

    Purpose

    Protein expression plasmid for 3xNLS SpCas9 in pET-21a backbone

    Depositor

    Scot Wolfe

    Article

    Wu et al Nat Med. 2019 May;25(5):776-783. doi: 10

    Insert

    3xNLS_SpCas9
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-Cre:GFP

    Plasmid

    #13776

    Purpose

    Mammalian expression of Cre recombinase fused to GFP

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

    Insert

    Cre-GFP fusion protein
    

    Use

    Cre/Lox

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 20, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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