We narrowed to 615 results for: vp64

1. pYPQ-dSpRY-Act3.0

   Plasmid

   #158416

   Purpose

   CRISPR-Act3.0 system containing dSpRYCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

   Insert

   dSpRY-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   July 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYPQ-dpcoCas9-Act3.0

   Plasmid

   #158408

   Purpose

   CRISPR-Act3.0 system containing dpcoCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

   Insert

   dpcoCas9-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. dxCas(3.6)-VPR

   Plasmid

   #108388

   Purpose

   Mammalian expression vector for xCas9 3.6 with VP64-p65-Rta (VPR) fused to its C-terminus

   Depositor

   David Liu

   Article

   Hu et al Nature. 2018 Feb 28. pii: nature26155. d

   Insert

   dxCas(3.6)-VPR
   

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   E108G, S217A, A262T, S409I, E480K, E543D, M694I, …

   Available Since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. ScFv-2ERT2-VPH

   Plasmid

   #120556

   Purpose

   Encode an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to 2 tandem ERT2, VP64, P65 and HSF1

   Depositor

   Yu Wang

   Article

   Lu et al Nucleic Acids Res. 2018 Mar 16;46(5):e25

   Insert

   scFvGCN4, GB1, ERT2, VP64, P65 and HSF1
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   hPGK

   Available Since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJZC42

   Plasmid

   #66564

   Purpose

   sgRNA + 1XPP7 with PCP-VP64 effector for mammalian cells, marked by mCherry

   Depositor

   Wendell Lim, Stanley Qi

   Article

   Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

   Inserts

   sgRNA + 1XPP7
   PCP-VP64 IRES mCherry
   

   Expression

   Mammalian

   Promoter

   CMV and U6

   Available Since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. ScFv-2ERT2-V

   Plasmid

   #120553

   Purpose

   3rd gen transfer vector. Encode an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to sfGFP, 2 tandem ERT2 and VP64.

   Depositor

   Yu Wang

   Article

   Lu et al Nucleic Acids Res. 2018 Mar 16;46(5):e25

   Insert

   scFvGCN4, sfGFP, GB1, ERT2, VP64
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   hPGK

   Available Since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMT1-13X-NOG(AtUBI10)-MTAP-LUC

   Plasmid

   #234371

   Purpose

   T-DNA vector for expression of the the MoonTag system under the control of the Arabidopsis Ubi10 promoter; Luciferase under control of transactivated promoter by two gRNAs, no gRNA included (NOG)

   Depositor

   Michael Smanski

   Article

   Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70

   Inserts

   NbGP41-sfGFP-VP64-GB1
   Luciferase
   dCas9-13XGP41
   

   Use

   Synthetic Biology

   Tags

   GB1, GP41, and sfGFP

   Expression

   Plant

   Promoter

   Arabidopsis Ubi10 and MTAP1 (synthetic)

   Available Since

   April 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYPQ-dzCas9-NG-Act3.0

   Plasmid

   #158415

   Purpose

   CRISPR-Act3.0 system containing dzCas9-NG-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

   Insert

   dzCas9-NG-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYPQ-dAaCas12b-Act3.0

   Plasmid

   #158413

   Purpose

   CRISPR-Act3.0 system containing dAaCas12b-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

   Insert

   dAaCas12b-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   July 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. L4219 TNFR2 CTEVp chain in PolyTX-mTagBFP2

    Plasmid

    #244177

    Purpose

    Constitutive expression of a MESA CTEVp chain encoding (N to C): TNFR2SS-3xFLAG-TNFR2ECD-mCD28TMD-CTEVp(190K)-PRS(M)-VP64-ZF6

    Depositor

    Joshua Leonard

    Article

    Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

    Insert

    MESA CTEVp chain with human TNFR2 SS and ECD, murine CD28 TMD, CTEVp (190K), TEVp PRS (M in P1'), and synTF (VP64-ZF6) (TNFRSF1B Synthetic, Human)
    

    Use

    Synthetic Biology

    Tags

    TNFR2 signal peptide - 3xFLAG

    Expression

    Mammalian

    Mutation

    CTEVp_190K

    Promoter

    hEF1a

    Available Since

    Oct. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMT1-13X-gRNA1-2(AtUBI10)-MTAP-LUC

    Plasmid

    #234370

    Purpose

    T-DNA vector for expression of the two protein components of the MoonTag system under the control of the Arabidopsis Ubi10 promoter; Luciferase under control of transactivated promoter by two gRNAs

    Depositor

    Michael Smanski

    Article

    Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70

    Inserts

    NbGP41-sfGFP-VP64-GB1
    Luciferase
    dCas9-13XGP41
    gRNA 1-1 and gRNA 1-2
    

    Use

    Synthetic Biology

    Tags

    GB1, GP41, and sfGFP

    Expression

    Plant

    Promoter

    Arabidopsis U6, Arabidopsis Ubi10, and MTAP1 (syn…

    Available Since

    April 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMT1-24X-gRNA1-2(AtUBI10)-MTAP-LUC

    Plasmid

    #234372

    Purpose

    T-DNA vector for expression of the two protein components of the MoonTag system under the control of the Arabidopsis Ubi10 promoter; Luciferase under control of transactivated promoter by two gRNAs

    Depositor

    Michael Smanski

    Article

    Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70

    Inserts

    NbGP41-sfGFP-VP64-GB1
    Luciferase
    dCas9-24XGP41
    gRNA 1-1 and gRNA 1-2
    

    Use

    Synthetic Biology

    Tags

    GB1, GP41, and sfGFP

    Expression

    Plant

    Promoter

    Arabidopsis U6, Arabidopsis Ubi10, and MTAP1 (syn…

    Available Since

    April 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAN1646

    Plasmid

    #127205

    Purpose

    pTet expression of Venus, Dual inducible expression of dCas9-VP64-mScarlet-degronSwitchA and KeyA-BFP-NLS

    Depositor

    Hana El-Samad

    Article

    Langan et al Nature. 2019 Jul 24. pii: 10.1038/s41586

    Insert

    p7x_tet-Venus-tADH1-pGal1-dCas9-VP64-Linker-mScarlet-Linker-degronSwitch_A_t12-tPGK1-pZ3-key_A_e18-mTagBFP2-NLS(SV40)-tSSA1
    

    Use

    Synthetic Biology

    Availability

    Academic Institutions and Nonprofits only

    

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14. PB-TRE-dCas9-VPR

    Plasmid

    #63800

    Purpose

    SP-dCas9-VPR with doxycycline-inducible expression

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

    Insert

    dCas9-VPR
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    VPR (VP64-p65-Rta)

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. mLama1 AAV sgRNA 1, 2, 5

    Plasmid

    #135339

    Purpose

    Expression plasmid of VP64-SadCas9 sgRNA 1, 2 and 3

    Depositor

    Ronald Cohn

    Article

    Kemaladewi et al Nature. 2019 Aug;572(7767):125-130. doi:

    Insert

    Lama1 VP64-dCas9 sgRNA Guides
    

    Use

    AAV

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAG414GPD-dCas9-VPR

    Plasmid

    #63801

    Purpose

    pAG414 series plasmid with GPD promoter driving expression of dCas9-VPR; cerevisiae vector

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

    Insert

    dCas9-VPR
    

    Use

    CRISPR and Synthetic Biology

    Tags

    VPR (VP64-p65-Rta)

    Expression

    Yeast

    Promoter

    GPD

    Available Since

    April 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. L4315 IL-10Ra CTEVp chain in PolyTX-mTagBFP2

    Plasmid

    #244171

    Purpose

    Constitutive expression of a MESA CTEVp chain encoding (N to C): IL10RaSS-3xFLAG-IL10RaECD-IL10RaTMD-CTEVp(190K)-PRS(M)-VP64-ZF6a

    Depositor

    Joshua Leonard

    Article

    Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

    Insert

    MESA CTEVp chain with human IL-10Ra SS, ECD and TMD, CTEVp (190K), TEVp PRS (M in P1'), and synTF (VP64-ZF6) (IL10RA Synthetic, Human)
    

    Use

    Synthetic Biology

    Tags

    IL10Ra signal peptide - 3xFLAG

    Expression

    Mammalian

    Mutation

    CTEVp_190K

    Promoter

    hEF1a

    Available Since

    Oct. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. L4804 VEGFR1 CTEVp chain in PolyTX-mTagBFP2

    Plasmid

    #244174

    Purpose

    Constitutive expression of a MESA CTEVp chain encoding (N to C): VEGFR1SS-3xFLAG-VEGFR1ECD-VEGFR1TMD-CTEVp(190K)-PRS(M)-VP64-ZF6

    Depositor

    Joshua Leonard

    Article

    Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

    Insert

    MESA CTEVp chain with human VEGFR1 SS, ECD and TMD, CTEVp (190K), TEVp PRS (M in P1'), and synTF (VP64-ZF6) (FLT1 Synthetic, Human)
    

    Use

    Synthetic Biology

    Tags

    VEGFR1 signal peptide - 3xFLAG

    Expression

    Mammalian

    Mutation

    CTEVp_190K

    Promoter

    hEF1a

    Available Since

    Oct. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. L4342 TNFR1 CTEVp chain in PolyTX-mTagBFP2

    Plasmid

    #244178

    Purpose

    Constitutive expression of a MESA CTEVp chain encoding (N to C): TNFR1SS-3xFLAG-TNFR1ECD(dMMP)-TNFR1TMD-CTEVp(75S)-PRS(M)-VP64-ZF6

    Depositor

    Joshua Leonard

    Article

    Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

    Insert

    MESA CTEVp chain with human TNFR1 SS, ECD (no MMP site) and TMD, CTEVp (190K), TEVp PRS (M in P1'), and synTF (VP64-ZF6) (TNFRSF1A Synthetic, Human)
    

    Use

    Synthetic Biology

    Tags

    TNFR1 signal peptide - 3xFLAG

    Expression

    Mammalian

    Mutation

    CTEVp_190K

    Promoter

    hEF1a

    Available Since

    Oct. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHR_Gal4UAS_dGFP_PGK_iRFP (pLZA042)

    Plasmid

    #203908

    Purpose

    Encodes dGFP downstream UAS promoter that's responsive to Gal4-VP64 activity in mammalian cells

    Depositor

    Jared Toettcher

    Article

    Zhu et al Nat Commun. 2023 Jun 2;14(1):3185. doi:

    Insert

    Gal4UAS_dGFP_PGK_iRFP
    

    Expression

    Mammalian

    Promoter

    5xUAS and PGK

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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