We narrowed to 14,560 results for: EGFP

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pFX-Lifeact-EGFP

Plasmid

#174477

Purpose

Express EGFP with actin-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

EGFP with actin-targeting sequence

Tags

Lifeact (N-terminal 17 amino acids from S. Cerevi…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19-POLBHR-eGFP

Plasmid

#176064

Purpose

Homology region +/- 800bp to the transcription start site of POLB, with EGFP inserted in-frame on the N-terminus of POLB, and a mutation in the PAM site used by POLBKO gRNA1 in POLB exon1

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

EGFP-PolB (POLB Human)

Tags

EGFP

Expression

Mammalian

Mutation

pUC with endogenous SapI mutated out, PAM mutatio…

Promoter

N/A

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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CTAG_eGFP-GHtag-HDEL

Plasmid

#136096

Purpose

FP with ER retention signal for C-term fusion with CDS12 with ER targeting signal peptide. Contains Gly-His tag

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CTAG_eGFP(noATG,noStop)-3xGly5xHys-HDEL

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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SITS-Cavin4b-EGFP

Plasmid

#176019

Purpose

Leishmania cell free expression of zebrafish Cavin4b-EGFP. Parton lab clone GRJ

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

Cavin4b (cavin4b Zebrafish)

Use

Leishmania cell free expression

Available Since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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SITS-EGFP-Bin1a

Plasmid

#176020

Purpose

Leishmania cell free expression of zebrafish EGFP-Bin1a. Parton lab clone GRQ

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

Bin1a

Use

Leishmania cell free expression

Tags

EGFP

Available Since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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SITS-EGFP-Bin1b

Plasmid

#176021

Purpose

Leishmania cell free expression of zebrafish EGFP-Bin1b. Parton lab clone GRS

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

Bin1b (bin1b Zebrafish)

Use

Leishmania cell free expression

Tags

EGFP

Available Since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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SITS-Cavin4a-EGFP

Plasmid

#176018

Purpose

Leishmania cell free expression of zebrafish Cavin4a-EGFP. Parton lab clone GRK

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

Cavin4a (cavin4a Zebrafish)

Use

Leishmania cell free expression

Available Since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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FKBP-EGFP-RanGAP*

Plasmid

#175238

Purpose

Bacterial expression and purification, low affinity SUMOylation substrate that can recruited to FRB with rapamycin, point mutation F562A reduces affinity for E2, increasing the Km

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

RANGAP1 (RANGAP1 Human)

Expression

Bacterial

Mutation

Amino acid 562 F to A

Promoter

tac

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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FKBP-EGFP-RanGAP

Plasmid

#175236

Purpose

Bacterial expression and purification, high affinity SUMOylation substrate that can recruited to FRB with rapamycin

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

RANGAP1 (RANGAP1 Human)

Expression

Bacterial

Promoter

tac

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pIVT-EGFP-ratCENP-C

Plasmid

#174445

Purpose

in vitro transcription template for rat CENP-C (N-ter EGFP tagged)

Depositor

Michael Lampson

Article

Kumon et al Cell. 2021 Sep 16;184(19):4904-4918.e11.

Insert

Centromere protein C

Use

In vitro transcription template

Tags

EGFP

Promoter

T7 SP6

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pIVT-EGFP-pumilioCENP-C

Plasmid

#174446

Purpose

in vitro transcription template for R. pumilio CENP-C (N-ter EGFP tagged)

Depositor

Michael Lampson

Article

Kumon et al Cell. 2021 Sep 16;184(19):4904-4918.e11.

Insert

Centromere protein C

Use

In vitro transcription template

Tags

EGFP

Promoter

T7 SP6

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pnCS_mEGFP-DSep2_Peanut-Strep

Plasmid

#174496

Purpose

bacterial co-expression of Drosophila Sep2 fused to monomeric EGFP and of Drosophila Peanut

Depositor

Manos Mavrakis

Article

Mavrakis et al Methods Cell Biol. 2016;136:199-220. doi

Inserts

Sep2 (Sep2 Fly)
peanut (pnut Fly)

Tags

Strep and mEGFP

Expression

Bacterial

Available Since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pRabies-EGFP-rtTAn

Plasmid

#172119

Purpose

Expression of EGFP and rtTA-InteinN in Rabies virus infected cells

Depositor

Chun Xu

Article

Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

Insert

EGFP, rtTAn-InteinN

Use

Rabies virus

Promoter

CMV

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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TetO::EGFP-C-Meikin

Plasmid

#174706

Purpose

Doxycycline inducible expression of human C-Meikin Separase cleavage fragment

Depositor

Iain Cheeseman

Article

Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8

Insert

Meikin

Tags

EGFP

Expression

Mammalian

Mutation

155-373

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-Flag-stm2585

Plasmid

#174397

Purpose

Native S. Typhimurium gene stm2585 (sarA/steE) with N-terminal GFP and Flag tags.

Depositor

Dennis Ko

Article

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Insert

sarA

Tags

EGFP and Flag

Expression

Mammalian

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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SITS-ccdb-EGFP

Plasmid

#118752

Purpose

Multisite gateway destination vector for 3' tagging with EGFP for cell free expression. Parton lab FEX

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

ccdb

Tags

EGFP

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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SITS-EGFP-ccdb

Plasmid

#118628

Purpose

Multisite gateway destination vector for 5' tagging with EGFP for cell free expression. Parton lab clone FEZ

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

ccdb

Tags

EGFP

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-Flag-stm2585_L139*

Plasmid

#174400

Purpose

S. Typhimurium gene stm2585 (sarA/steE) truncated after 139th amino acid, which removes the stretch homologous to gp130

Depositor

Dennis Ko

Article

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Insert

sarA

Tags

EGFP and Flag

Expression

Mammalian

Mutation

L139* (Truncation after 139th amino acid in sarA)

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-Flag-stm2585_S130A

Plasmid

#174399

Purpose

Native S. Typhimurium gene stm2585 (sarA/steE) with N-terminal GFP and Flag tags and its GSK3-binding SxxxSP motif mutated to SxxxAP

Depositor

Dennis Ko

Article

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Insert

sarA

Tags

EGFP and Flag

Expression

Mammalian

Mutation

S130A

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-Flag-stm2585_Y167F

Plasmid

#174398

Purpose

Native S. Typhimurium gene stm2585 (sarA/steE) with N-terminal GFP and Flag tags and its STAT3-binding YxxQ motif mutated to FxxQ

Depositor

Dennis Ko

Article

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Insert

sarA

Tags

EGFP and Flag

Expression

Mammalian

Mutation

Y167F

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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