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pAAV-hSyn-mCherry.3xFLAG-WPRE

Plasmid

#127861

Purpose

pAAV plasmid expressing an mCherry.3xFLAG fusion protein under the hSyn promoter

Depositor

Florian Freudenberg

Article

Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

Insert

mCherry

Use

AAV

Tags

3xFLAG

Promoter

hSyn

Available Since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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Hy_pMT dati

Plasmid

#91800

Purpose

Expresses the Drosophila dati circular RNA

Depositor

Jeremy Wilusz

Article

Liang et al Mol Cell. 2017 Nov 9. pii: S1097-2765(17

Insert

dati (dati Fly)

Expression

Insect

Promoter

Metallothionein Promoter (pMT)

Available Since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pXNgate21-3HA

Plasmid

#105078

Purpose

Yeast mating-based Split Ubiquitin Assay, prey vector for in vivo cloning

Depositor

Wolf Frommer

Article

Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

Type

Empty backbone

Tags

NubG

Expression

Yeast

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pSUM-Kan-MCS2

Plasmid

#109289

Purpose

Expresses high levels of protein in mycobacteria

Depositor

Nicolai van Oers

Article

Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

Type

Empty backbone

Expression

Bacterial

Mutation

pSUM-kan-MCS1-gfp was modified to reorient the Ba…

Available Since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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pCS2 Flag Smad3 EPSM V179T

Plasmid

#26998

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Mutation

T179V S204A S208A S213A; V179T

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pME DN-E-cadherin

Plasmid

#135209

Purpose

Dominate Negative zebrafish e-cadherin label

Depositor

David Tobin

Article

Cronan et al Immunity. 2016 Oct 18;45(4):861-876. doi

Insert

CDH1

Use

Synthetic Biology

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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PX458_TFE3_2

Plasmid

#86326

Purpose

Encodes gRNA for 3' target of human TFE3

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against TFE3 (TFE3 Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCASP-SptP120-3G124-HilA

Plasmid

#153323

Purpose

Triggers secretion of SptP120 fused to the 3G124nc anti-GFP DARPin from Salmonella upon induction with arabinose

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

SptP120-3G124 DARPin

Use

Salmonella expression

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pDC184

Plasmid

#70055

Purpose

Co-expresses Syt1-SNARE37aa-linker complex with p045

Depositor

Axel Brunger

Article

Zhou et al Nature. 2015 Sep 3;525(7567):62-7. doi:

Inserts

His tagged Synaptobrevin 28-89
Syntaxin 191-256

Tags

10xHis

Expression

Bacterial

Promoter

T7

Available Since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pHS1528

Plasmid

#205982

Purpose

U6-MWEE01000013 Cas5-HNH DR-BpiI-DR

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas5-HNH DR-BpiI-DR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE N-mRuby3 (from SARS-CoV-2) IRES puro

Plasmid

#170466

Purpose

The plasmid codes for the Nucleoprotein (N) of SARS-CoV-2 fused to a mRuby3 fluorescent protein in its C-terminus. Inserted in a lentiviral backbone. Puromycin resistance

Depositor

Raphael Gaudin

Article

Bakhache et al bioRxiv 2021

Insert

Nucleocapsid

Use

Lentiviral

Tags

mRuby3

Expression

Mammalian

Promoter

EF1

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX2T VPS4B, residues 1-86

Plasmid

#180639

Purpose

Bacterial expression for VPS4B MIT domain, residues 1-86. Has N-terminal GST tag. Internal ID: WISP04-155.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

VPS4B (VPS4B Human)

Tags

GST

Expression

Bacterial

Mutation

Residues 1-86

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCB128

Plasmid

#205137

Purpose

Expression of mCherry-2xFYVE in Dictyostelium

Depositor

Jason King

Article

Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

Insert

2xFYVE (from human Hrs) (HGS Synthetic)

Use

Dictyostelium expression

Tags

mCherry

Available Since

Oct. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pFETCh_CIC_iso1

Plasmid

#135728

Purpose

Donor vector for 3' FLAG tag of human CIC_iso1

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

CIC_iso1 Homology arms (CIC Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pTYF-sGFAP-LOx-IRES-tdTomato

Plasmid

#166919

Purpose

Express the bacterial lactate oxidase (LOx) enzyme in astrocytes

Depositor

Sergey Kasparov

Article

Vaccari Cardoso et al Glia. 2021 Jan 5. doi: 10.1002/glia.2396

Insert

Lactate oxidase

Use

Lentiviral

Tags

tdTomato

Expression

Mammalian

Promoter

sGFAP

Available Since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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pBR322-Pdp1_NTD-ZFN_L-HiBiT

Plasmid

#198319

Purpose

Loads HiBiT-tagged L fragment of a zinc finger nuclease specific for human AAVS1 locus using Pdp1 packaging domain

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

Pdp1_NTD-ZFN_L-HiBiT

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pCI Flag Smad3 LC EPSM

Plasmid

#27038

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Tags

Flag

Expression

Mammalian

Mutation

aa 133-425; T179V S204A S208A S213A

Available Since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only
MDO2-c003

Plasmid

#173105

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

MACROD2 (MACROD2 Human)

Expression

Bacterial

Mutation

K7-D243

Available Since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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MDO2-c005

Plasmid

#173106

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

MACROD2 (MACROD2 Human)

Tags

Nanoluc

Expression

Bacterial

Mutation

K7-D243

Available Since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pY019 (pcDNA3.1-hPmCpf1)

Plasmid

#69991

Purpose

Expresses humanized PmCpf1

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

hPmCpf1

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only