We narrowed to 5,135 results for: codon optimized

1. Nsp12-EGFP

   Plasmid

   #165116

   Purpose

   mammalian expression and localization

   Depositor

   Bruno Antonny

   Article

   Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

   Insert

   Nsp12 (ORF1ab codon optimized SARS-COV-2)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. ATG-2460

   Plasmid

   #108713

   Purpose

   EF1-CBR2opt-T2A-copGFP (construct used for lentiviral transduction)

   Depositor

   Keith Wood

   Article

   Hall et al Nat Commun. 2018 Jan 9;9(1):132. doi: 10

   Insert

   CBR2opt-copGFP fusion
   

   Use

   Lentiviral

   Tags

   CBR2opt fused to copGFP

   Mutation

   Promega used directed evolution to create CBR (Cl…

   Promoter

   elongation factor-1

   Available Since

   Oct. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5-FRT-TO-Nsp16-HF

   Plasmid

   #157725

   Purpose

   mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp16 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   Nsp16-HF (ORF1ab SARS-CoV-2)
   

   Tags

   His8-Flag

   Expression

   Mammalian

   Mutation

   codon optimized

   Available Since

   Aug. 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. Nsp8-EGFP

   Plasmid

   #165113

   Purpose

   mammalian expression and localization

   Depositor

   Bruno Antonny

   Article

   Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

   Insert

   Nsp8 (ORF1ab codon optimized SARS-COV-2)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pC001 - huLshC2C2-MBP for bacterial expression

   Plasmid

   #79150

   Purpose

   Expresses human codon-optimized LshC2c2 for purification in E. coli

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

   Insert

   C2c2
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. VPS13B^GFP

   Plasmid

   #224588

   Purpose

   codon optimized GFP tagged VPS13B for expression in mammalian cells

   Depositor

   Pietro De Camilli

   Article

   Ugur et al bioRxiv [Preprint]. 2023 Dec 18:2023.12.

   Insert

   VPS13B^GFP (VPS13B Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Dec. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. GB1-A11(2-196, D40G)-Strep

   Plasmid

   #196210

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, D40G)-Strep.

   Depositor

   Lalit Deshmukh

   Article

   Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

   Insert

   Annexin A11 (ANXA11 Human)
   

   Tags

   GB1-6xHis-TEV and Strep-II tag

   Expression

   Bacterial

   Mutation

   D40G

   Available Since

   July 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEABR NA 3C FLAG SA

   Plasmid

   #234987

   Purpose

   For production of Extracellular Vesicles (EVs), with the transmembrane region of Neuraminidase protein fused to Streptavidin on the vesicles surface

   Depositor

   Monica Gonzalez-Magaldi, Daniel Leahy

   Article

   Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

   Insert

   EABR-NA
   

   Tags

   HRV 3C site, FLAG, Strep-Tactin (SA)

   Expression

   Mammalian

   Mutation

   In the Streptavidin coding sequence Glu44Val/Ser4…

   Available Since

   April 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. TccCas13a Twin SUMO

   Plasmid

   #199754

   Purpose

   Expresses codon optimized thermostabile TccCas13a in E.coli

   Depositor

   Magdy Mahfouz

   Article

   Mahas et al Proc Natl Acad Sci U S A. 2022 Jul 12;11

   Insert

   TccCas13a
   

   Tags

   SUMO

   Expression

   Bacterial

   Promoter

   LacI

   Available Since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. Cas12j-8-puro-crRNA

    Plasmid

    #194965

    Purpose

    The plasmid expresses human codon-optimized Cas12j-8, crRNA expression elements and puromycin resistence gene.

    Depositor

    Yongming Wang

    Article

    Wang et al Sci Adv. 2023 Feb 10;9(6):eabo6405. doi:

    Type

    Empty backbone

    Use

    AAV

    Expression

    Mammalian

    Available Since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. GB1-A11(2-196, G38R)-Strep

    Plasmid

    #196209

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, G38R)-Strep.

    Depositor

    Lalit Deshmukh

    Article

    Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

    Insert

    Annexin A11 (ANXA11 Human)
    

    Tags

    GB1-6xHis-TEV and Strep-II tag

    Expression

    Bacterial

    Mutation

    G38R

    Available Since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRK5-FLAG-SLC38A9.1

    Plasmid

    #71855

    Purpose

    overexpression

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Jan 9;347(6218):188-94. do

    Insert

    SLC38A9.1 (SLC38A9 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    CMV

    Available Since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEN114 - pCAGGS-Tir1-V5-BpA-Frt-PGK-EM7-PuroR-bpA-Frt-Rosa26

    Plasmid

    #92143

    Purpose

    Targeting vector to introduce an osTir1 expressing cassette at the mouse Rosa26 locus using Puromycin selection. Auxin-inducible degron system.

    Depositor

    Benoit Bruneau

    Article

    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

    Insert

    osTir1
    

    Use

    Mouse Targeting

    Tags

    V5 tag

    Expression

    Mammalian

    Available Since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET42b-ABE7.10

    Plasmid

    #120398

    Purpose

    Expresses ABE7.10-NLS with an N-terminal His Tag (His6) for bacterial expression

    Depositor

    Junjiu Huang

    Article

    Liang et al Nat Commun. 2019 Jan 8;10(1):67. doi: 10

    Insert

    ABE7.10
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Mammalian codon-optimized

    Promoter

    T7

    Available Since

    April 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3/er-(n2)oxStayGold(c4)

    Plasmid

    #185822

    Purpose

    A soluble photostable fluorescent marker of the ER lumen. The StayGold gene has no codon optimization for mammalian expression.

    Depositor

    Atsushi Miyawaki

    Article

    Hirano et al Nat Biotechnol. 2022 Apr 25. pii: 10.103

    Insert

    er-(n2)oxStayGold(c4)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET-32a(+)-TGFB1m

    Plasmid

    #120286

    Purpose

    Produce human recombinant mTGFB1 in DE3 cells

    Depositor

    Paul Burridge

    Article

    Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

    Insert

    monomeric Transforming Growth Factor-β1
    

    Tags

    6x His and S-Tag

    Expression

    Bacterial

    Mutation

    Codon optimized for E. Coli production

    Promoter

    T7 Promotor

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. ORF3a-EGFP

    Plasmid

    #165121

    Purpose

    mammalian expression and localization

    Depositor

    Bruno Antonny

    Article

    Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

    Insert

    ORF3a (ORF3a codon optimized SARS-COV-2)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGES401

    Plasmid

    #190198

    Purpose

    For CRISPR-Cas9 mediated multiple gene editing in soybean, single transcript unit system driven by soybean elongation factor 1A promoter (pM4), Basta selection

    Depositor

    Yuefeng Guan

    Article

    Bai et al Mol Plant. 2022 Jul 4;15(7):1081-1083. d

    Insert

    spCas9
    

    Use

    CRISPR

    Tags

    3xFlag

    Expression

    Plant

    Mutation

    plant-codon optimized

    Promoter

    Glycine max elongation factor 1A(pM4)

    Available Since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBUN6A11

    Plasmid

    #50579

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-VP64, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Inserts

    dCas9-VP64
    gRNA scaffold
    

    Use

    CRISPR; Plant expression

    Tags

    3xFLAG, HA, and NLS

    Mutation

    dCas9 = defective in DNA cleavage; 4×minimal VP16…

    Promoter

    OsU3p and Ubi1p

    Available Since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. B-Sniper SpCas9

    Plasmid

    #207361

    Purpose

    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-Sniper SpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    F539S, M763I, K890N, amino acids 1005-1013 replac…

    Promoter

    CBh

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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