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We narrowed to 4,955 results for: codon optimized

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Showing: 3581 - 3600 of 4955 results

  1. pAAV-CaMKII-Cx35-M1-FLAG-T2A-mCherry

    Plasmid
    #255639
    Purpose
    Encodes M. americana Cx35, codon optimized for mouse expression and c-terminally fused FLAG tag, T2A self-cleaving peptide and mCherry. Mutation of K221E. Expression driven by CaMKII promoter.
    Depositor
    Kafui Dzirasa
    Publication
    Ransey et al Nature. 2026 May 13. doi: 10.1038/s41586
    Insert
    Cx35
    Use
    AAV
    Tags
    FLAG-T2A-mCherry
    Mutation
    K221E
    Promoter
    CaMKII
    Available since
    May 26, 2026
    Availability
    Academic Institutions and Nonprofits only
    255639-plasmid-map-sequence-id-511283

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  2. pAAV-EF1⍺-DIO-Cx34.7-M1-mEmerald

    Plasmid
    #255634
    Purpose
    Encodes M. americana Cx34.7, codon optimized for mouse expression, with 8 amino acid linker and c-terminally fused mEmerald tag. Mutations of E214K and E223K. Expression driven by EF1a promoter.
    Depositor
    Kafui Dzirasa
    Publication
    Ransey et al Nature. 2026 May 13. doi: 10.1038/s41586
    Insert
    Cx34.7
    Use
    AAV
    Tags
    mEmerald
    Mutation
    E214K,E223K
    Promoter
    EF1a
    Available since
    May 26, 2026
    Availability
    Academic Institutions and Nonprofits only
    255634-plasmid-map-sequence-id-511281

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  3. pAAV-hSyn-Cx35-M1-FLAG-T2A-mCherry

    Plasmid
    #255640
    Purpose
    Encodes M. americana Cx35, codon optimized for mouse expression and c-terminally fused FLAG tag, T2A self-cleaving peptide and mCherry. Mutation of K221E. Expression driven by hSyn promoter.
    Depositor
    Kafui Dzirasa
    Publication
    Ransey et al Nature. 2026 May 13. doi: 10.1038/s41586
    Insert
    Cx35
    Use
    AAV
    Tags
    FLAG-T2A-mCherry
    Mutation
    K221E
    Promoter
    hSyn
    Available since
    May 26, 2026
    Availability
    Academic Institutions and Nonprofits only
    255640-plasmid-map-sequence-id-511280

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  4. pMXs-IRES-blast 3xFlag-GFP-SLC45A1(E774A, L778A, L779A)

    Plasmid
    #228523
    Purpose
    SLC45A1(E774A, L778A, L779A) codon-optimized orf with n-terminal 3xFlag and GFP in pMXs-IRES-Blast vector
    Depositor
    Monther Abu-Remaileh
    Publication
    Ghoochani et al Cell. 2026 Feb 5;189(3):765-782.e31. doi
    Insert
    SLC45A1 (SLC45A1 Human)
    Use
    Retroviral
    Tags
    Flag and GFP
    Mutation
    At position 774, glutamic acid (E) is replaced by…
    Available since
    April 21, 2026
    Availability
    Academic Institutions and Nonprofits only
    228523-plasmid-map-sequence-id-454855

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  7. EFS-SpdCas9-Dnmt3A/3L-V1

    Plasmid
    #222498
    Purpose
    Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V1 (R887E) followed by P2A-mCherry
    Depositor
    Isaac Hilton
    Publication
    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021
    Insert
    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V1 (R887E) engineered fusion
    Use
    Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; R887E in Dnmt3A
    Promoter
    EFS
    Available since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    222498-plasmid-map-sequence-id-441135

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  8. EFS-SpdCas9-Dnmt3A/3L-V2

    Plasmid
    #222499
    Purpose
    Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V2 (E814G) followed by P2A-mCherry
    Depositor
    Isaac Hilton
    Publication
    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021
    Insert
    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V2 (E814G) engineered fusion
    Use
    Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E814G in Dnmt3A
    Promoter
    EFS
    Available since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    222499-plasmid-map-sequence-id-441136

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  9. TRE3GV-SpdCas9-Dnmt3A/3L-V1

    Plasmid
    #222503
    Purpose
    Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V1 (R887E) and hPGK promoter driven mCherry
    Depositor
    Isaac Hilton
    Publication
    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021
    Inserts
    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V1 (R887E) engineered fusion
    mCherry
    Use
    Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; R887E in Dnmt3A
    Promoter
    TRE3GV and hPGK
    Available since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    222503-plasmid-map-sequence-id-441222

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  10. TRE3GV-SpdCas9-Dnmt3A/3L-V3

    Plasmid
    #222506
    Purpose
    Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V3 (R887E and E814G) and hPGK promoter driven mCherry
    Depositor
    Isaac Hilton
    Publication
    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021
    Inserts
    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V3 (R887E and E814G) engineered fusion
    mCherry
    Use
    Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; R887E and E814…
    Promoter
    TRE3GV and hPGK
    Available since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    222506-plasmid-map-sequence-id-441224

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  11. TRE3GV-SpdCas9-Dnmt3A/3L-catΔ

    Plasmid
    #222508
    Purpose
    Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant catΔ (C706A and R832E) and hPGK promoter driven mCherry
    Depositor
    Isaac Hilton
    Publication
    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021
    Inserts
    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant catΔ (C706A and R832E) engineered fusion
    mCherry
    Use
    Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; C706A and R832…
    Promoter
    TRE3GV and hPGK
    Available since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    222508-plasmid-map-sequence-id-441225

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  12. pX330-U6-(sgRNA-T2)-Chimeric_BB-CBh-hSpCas9

    Plasmid
    #223323
    Purpose
    Human codon optimized Cas9 expressing plasmid that carries the sgRNA T2 from Mali et al., 2013 (10.1126/science.1232033).
    Depositor
    Jonathan Arias
    Publication
    Petruskeviciute et al Stem Cell Res. 2024 Dec 12;82:103628. do
    Insert
    SpCas9 and sgRNA-T2 (cas9 )
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Dec. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    223323-plasmid-map-sequence-id-447744

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  13. HEpl016

    Plasmid
    #179991
    Purpose
    pCBh_3xFLAG_NLS_SiCas12i_NLS_pA_pCMV_mCherry_pU6_crRNA_BbsI: vector for encoding a human codon-optimized SiCas12i driven by CBh promoter and tagmCherry driven by CMV promoter
    Depositor
    Yingsi Zhou
    Publication
    Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.
    Insert
    SiCas12i
    Tags
    3xFlag
    Expression
    Mammalian
    Available since
    April 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    179991-plasmid-map-sequence-id-358514

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  20. pCJ205

    Plasmid
    #162676
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating C224A an
    Depositor
    Gregg Beckham
    Publication
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Tags
    His
    Expression
    Bacterial
    Mutation
    C224A, C529A; codon optimized for expression in E…
    Promoter
    T7/lac
    Available since
    Feb. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    162676-plasmid-map-sequence-id-320828

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Showing: 3581 - 3600 of 4955 results