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MKC84_HBB(HBA1reg-HBA1)

Plasmid

#232410

Purpose

AAV production plasmid for HBA1 UTRs vector from Fig. 3 that mediates HDR at HBB locus using HBB sg7 gRNA. HBA gene includes HBA1 exons and introns; HBA1 UTRs flank HBA1 cassette.

Depositor

Kyle Cromer

Article

Chu et al bioRxiv [Preprint]. 2024 May 7:2023.09.0

Insert

Hemoglobin Subunit Alpha 1 (HBA1 Human)

Use

AAV

Expression

Mammalian

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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MKC83_HBB(HBBreg-HBA1)

Plasmid

#232409

Purpose

AAV production plasmid for HBB UTRs vector from Fig. 3 that mediates HDR at HBB locus using HBB sg7 gRNA. HBA gene includes HBA1 exons and introns; HBB UTRs flank HBA1 cassette.

Depositor

Kyle Cromer

Article

Chu et al bioRxiv [Preprint]. 2024 May 7:2023.09.0

Insert

Hemoglobin Subunit Alpha 1 (HBA1 Human)

Use

AAV

Expression

Mammalian

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-multi-CRISPR-sgRipk3_#2-puro

Plasmid

#231982

Purpose

Knockout mouse Ripk3

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

sgRNA with Cas9 with puromycin resistance (Ripk3 Mouse)

Use

CRISPR and Lentiviral

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-multi-CRISPR-sgRipk3_#1-puro

Plasmid

#231981

Purpose

Knockout mouse Ripk3

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

sgRNA with Cas9 with puromycin resistance (Ripk3 Mouse)

Use

CRISPR and Lentiviral

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-multi-CRISPR-sgMlkl_#2-puro

Plasmid

#231980

Purpose

Knockout mouse Mlkl

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

sgRNA with Cas9 with puromycin resistance (Mlkl Mouse)

Use

CRISPR and Lentiviral

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-multi-CRISPR-sgMlkl_#1-puro

Plasmid

#231979

Purpose

Knockout mouse Mlkl

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

sgRNA with Cas9 with puromycin resistance (Mlkl Mouse)

Use

CRISPR and Lentiviral

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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phH1-GBX2-gRNA

Plasmid

#192510

Purpose

Contains gRNA for GBX2

Depositor

Mark Denham

Article

Maimaitili et al Nat Commun. 2023 Dec 5;14(1):7871. doi:

Insert

GBX2 (GBX2 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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phU6-CDX4-gRNA

Plasmid

#192511

Purpose

Contains gRNA for CDX4

Depositor

Mark Denham

Article

Maimaitili et al Nat Commun. 2023 Dec 5;14(1):7871. doi:

Insert

CDX4 (CDX4 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pmU6-CDX1-gRNA

Plasmid

#192512

Purpose

Contains gRNA for CDX1

Depositor

Mark Denham

Article

Maimaitili et al Nat Commun. 2023 Dec 5;14(1):7871. doi:

Insert

CDX1 (CDX1 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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ph7SK-CDX2-gRNA

Plasmid

#192513

Purpose

Contains gRNA for CDX2

Depositor

Mark Denham

Article

Maimaitili et al Nat Commun. 2023 Dec 5;14(1):7871. doi:

Insert

CDX2 (CDX2 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-Basic-ZC3H12D_PromoterMUT

Plasmid

#153067

Purpose

ZC3H12D promoter region carrying mutations in BHLHE40 binding sites, cloned upstream of luciferase reporter

Depositor

Silvia Monticelli

Article

Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

Insert

ZC3H12D promoter region mutated in BHLHE40 binding sites (ZC3H12D Human)

Use

Luciferase

Expression

Mammalian

Mutation

Mutagenesis to disrupt 3 BHLHE4040 binding sites.…

Promoter

None

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-hPC1_MTS_L4139P-eGFP-3HA

Plasmid

#108404

Purpose

Express putative mitochondrial targeting sequence of human PC1 from amino acid 4129 to 4157 with a likely pathogenic point mutation.

Depositor

Gregory Germino

Article

Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

Insert

PKD1 (PKD1 Human)

Tags

3xHA and eGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-hPC1_MTS_V4146I-eGFP

Plasmid

#108405

Purpose

Express putative mitochondrial targeting sequence of human PC1 from amino acid 4129 to 4157 with a likely non-pathogenic variant.

Depositor

Gregory Germino

Article

Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

Insert

PKD1 (PKD1 Mouse)

Tags

3xHA and eGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 2, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-hPC1_MTS_L4137P-eGFP

Plasmid

#108403

Purpose

Express putative mitochondrial targeting sequence of human PC1 from amino acid 4129 to 4157 with a likely pathogenic point mutation.

Depositor

Gregory Germino

Article

Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

Insert

PKD1 (PKD1 Mouse)

Tags

3xHA and eGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 2, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-hPC1_MTS-eGFP

Plasmid

#108402

Purpose

Express putative mitochondrial targeting sequence of human PC1 from amino acid 4129 to 4157

Depositor

Gregory Germino

Article

Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

Insert

PKD1 (PKD1 Mouse)

Tags

3xHA and eGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 2, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223_PSG2_WT

Plasmid

#81917

Purpose

Gateway Donor vector containing PSG2 ,part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PSG2 (PSG2 Human)

Use

Gateway entry vector

Mutation

V20L; p.333_335del-NPT-ins-IQH

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SMR3A_WT

Plasmid

#81887

Purpose

Gateway Donor vector containing SMR3A , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SMR3A (SMR3A Human)

Use

Gateway entry vector

Mutation

C42R; P132L

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_C1orf150_WT

Plasmid

#81842

Purpose

Gateway Donor vector containing C1ORF150 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

C1ORF150 (GCSAML Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SPAG8_WT

Plasmid

#81848

Purpose

Gateway Donor vector containing SPAG8 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SPAG8 (SPAG8 Human)

Use

Gateway entry vector

Mutation

E2D; E5G; P226L

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CPXCR1_WT

Plasmid

#81819

Purpose

Gateway Donor vector containing CPXCR1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CPXCR1 (CPXCR1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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