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Showing: 7241 - 7280 of 7585 results

  4. TFORF3265

    Plasmid
    #144741
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ARNTL2 (BMAL2 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144741-plasmid-map-sequence-id-404345

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  6. pcDNA3.1 Flag Cl-sensitive YFP hNKCC1 WT (NT13)

    Plasmid
    #49060
    Purpose
    Expresses human NKCC1 with an N-terminal 3xFlag-YFP-(chloride-sensing) tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. Native hNKCC1 amino acid sequence
    Depositor
    Biff Forbush
    Article
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and YFP (chloride-sensitive)
    Expression
    Mammalian
    Mutation
    Cl-sensitive YFP (EYFP/ V163S A206K) in hNKCC1 in…
    Promoter
    CMV
    Available since
    Nov. 7, 2013
    Availability
    Academic Institutions and Nonprofits only
    49060-plasmid-map-sequence-id-72178

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  9. Cav2.2 HA E1658A (EIVA) pcDNA3

    Plasmid
    #206114
    Purpose
    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and an E1658A mutation in the P loop of Domain IV which reduces trafficking to the cell surface
    Depositor
    Annette Dolphin
    Article
    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi
    Insert
    cacna1b (CACNA1B Rabbit)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    E1658A
    Promoter
    CMV
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    206114-plasmid-map-sequence-id-415366

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  12. TFORF3264

    Plasmid
    #144740
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ARNTL2 (BMAL2 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    144740-plasmid-map-sequence-id-364397

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  13. TFORF2653

    Plasmid
    #144059
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ARNTL2 (BMAL2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    144059-plasmid-map-sequence-id-400561

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  14. pcDNA3.1 Flag YFP hNKCC1 HA-ECL4 (NT935)

    Plasmid
    #49065
    Purpose
    Expresses human NKCC1 mutant with HA tag inserted into extracellular loop #4 and N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    2xHA epitope in ECL4 inserted at aa574 in hNKCC1 …
    Promoter
    CMV
    Available since
    Nov. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    49065-plasmid-map-sequence-id-72190

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  15. pcDNA3.1 Flag YFP hNKCC1 HA-ECL2 (NT931)

    Plasmid
    #49063
    Purpose
    Expresses human NKCC1 mutant with HA tag inserted into extracellular loop #2 and an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    2xHA epitope in ECL2 inserted at aa398 in hNKCC1 …
    Promoter
    CMV
    Available since
    Nov. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    49063-plasmid-map-sequence-id-72185

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  16. pcDNA3.1 Flag YFP hNKCC1 HA-ECL3 (NT933)

    Plasmid
    #49064
    Purpose
    Expresses human NKCC1 mutant with HA tag inserted into extracellular loop #3 and N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    2xHA epitope in ECL3 inserted at aa460 in hNKCC1 …
    Promoter
    CMV
    Available since
    Nov. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    49064-plasmid-map-sequence-id-72188

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  17. pcDNA3.1 Flag YFP hNKCC1 extracellular-cysteineless (NT864)

    Plasmid
    #49069
    Purpose
    Expresses human NKCC1 mutant lacking extracellular cysteine residues and with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    C563S,C568S,C577S,C582S in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Nov. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    49069-plasmid-map-sequence-id-72196

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  20. pIRES-mCerulean-FRB-Rac1/N12-Rac1/13C(Q61L)-FKBP-mVenus-CAAX

    Plasmid
    #214280
    Purpose
    Express split-Rac1 fragments that are fused with CIDs and FPs
    Depositor
    Cliff Stains
    Article
    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202
    Inserts
    Split-Rac1 fragments fused with CIDs and FPs (RAC1 Human)
    Split-Rac1 fragments fused with CIDs and FPs (RAC1 Human)
    Use
    Tags
    mCerulean (on insert 1) and mVenus (on insert 2)
    Expression
    Mammalian
    Mutation
    Rac1-Q61L
    Promoter
    CMV
    Available since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    214280-plasmid-map-sequence-id-422247

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  21. pIRES-mCerulean-FRB-Rac1/N12-Rac1/13C(T17N)-FKBP-mVenus-CAAX

    Plasmid
    #214281
    Purpose
    Express split-Rac1 fragments that are fused with CIDs and FPs
    Depositor
    Cliff Stains
    Article
    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202
    Inserts
    Split-Rac1 fragments fused with CIDs and FPs (RAC1 Human)
    Split-Rac1 fragments fused with CIDs and FPs (RAC1 Human)
    Use
    Tags
    mCerulean (N terminal on insert 1) and mVenus (on…
    Expression
    Mammalian
    Mutation
    Rac1-T17N
    Promoter
    CMV
    Available since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    214281-plasmid-map-sequence-id-422246

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  22. Cav2.2 HA EIVK (E1658K) pcDNA3

    Plasmid
    #206066
    Purpose
    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and an E1658K mutation in the P loop of Domain IV which abolishes trafficking to the cell surface
    Depositor
    Annette Dolphin
    Article
    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi
    Insert
    cacna1b (CACNA1B Rabbit)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    E1658K
    Promoter
    CMV
    Available since
    Oct. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    206066-plasmid-map-sequence-id-410269

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  23. TFORF2654

    Plasmid
    #144060
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ARNTL2 (BMAL2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    144060-plasmid-map-sequence-id-400507

    Enlarge

  24. pCR2.1_HBB_A

    Plasmid
    #87848
    Purpose
    pCR2.1-based plasmid holding a 328-bp fragment containing the exon-1-exon-2 splice border of aberrant HBB[IVSI-110(G>A)] human β-globin mRNA
    Depositor
    Marina Kleanthous
    Article
    Patsali et al Int J Mol Sci. 2020 Sep 11;21(18). pii:
    Insert
    Homo sapiens hemoglobin subunit beta (HBB), Aberrant cDNA fragment (Exon1/19nt mispliced Intron1 + Exon2) (HBB Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    HBB(IVSI-110) β-thalassaemia misplicing mutation …
    Promoter
    Available since
    Oct. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    87848-plasmid-map-sequence-id-313713

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  27. CFP(1-158)-RGS7t

    Plasmid
    #55761
    Purpose
    An amino-terminal CFP Fragment was fused to residues 202-477 of RGS7. When co-expressed with a carboxyl terminal fragment of CFP fused to Gbeta-5, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Article
    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep
    Insert
    RGS7(202-477)-CFP(1-158) (RGS7 Aequorea victoria, Human)
    Use
    Tags
    CFP(1-158) was fused to the amino terminus of RGS…
    Expression
    Mammalian
    Mutation
    The RGS sequence amino terminal to the GGL domain…
    Promoter
    CMV
    Available since
    Aug. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    55761-plasmid-map-sequence-id-89566

    Enlarge

  28. pcDNA3.1 Flag YFP hNKCC1 intracellular-cysteineless (NT855)

    Plasmid
    #49080
    Purpose
    Expresses human NKCC1 mutant lacking cysteine residues within intracellular loops and with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    C791S, C820S, C1052S in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Nov. 22, 2013
    Availability
    Academic Institutions and Nonprofits only
    49080-plasmid-map-sequence-id-72210

    Enlarge

  29. pcDNA3.1 Flag YFP hNKCC1 ECL4 deletion (NT865)

    Plasmid
    #49070
    Purpose
    Expresses human NKCC1 truncation mutant lacking extracellular loop #4 (aa562-582) and with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    ECL4 deletion of amino acids 562-582 in hNKCC1 in…
    Promoter
    CMV
    Available since
    Nov. 19, 2013
    Availability
    Academic Institutions and Nonprofits only
    49070-plasmid-map-sequence-id-72197

    Enlarge

  30. pcDNA3.1 Flag YFP hNKCC1 cysteineless (NT868)

    Plasmid
    #49079
    Purpose
    Expresses human NKCC1 mutant lacking cysteine residues and with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    C295A, C543M, C563S,C568S,C577S,C582S, C630S, C72…
    Promoter
    CMV
    Available since
    Nov. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    49079-plasmid-map-sequence-id-72208

    Enlarge

  31. pcDNA3.1 Flag YFP hNKCC1 TM-cysteineless (NT859)

    Plasmid
    #49078
    Purpose
    Expresses human NKCC1 mutant lacking cysteine residues within transmembrane domains and with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic,containing convenient restriction sites
    Depositor
    Biff Forbush
    Article
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    C295A, C543M, C630S, C723S, C724V in hNKCC1 in NT…
    Promoter
    CMV
    Available since
    Nov. 7, 2013
    Availability
    Academic Institutions and Nonprofits only
    49078-plasmid-map-sequence-id-72207

    Enlarge

  32. pUCmini-iCAP-AAV.CAP-B10

    Plasmid
    #175004
    Purpose
    non-standard AAV2 rep-AAV.CAP-B10 cap plasmid with AAV cap expression controlled by a tTA-TRE amplifcation system
    Depositor
    Viviana Gradinaru
    Article
    Goertsen et al Nat Neurosci. 2022 Jan;25(1):106-115. do
    Insert
    Synthetic construct isolate AAV.CAP-B10 VP1 gene
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    7 amino acid substitution between VP1 452 and VP1…
    Promoter
    p41
    Available since
    Nov. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    175004-plasmid-map-sequence-id-347131

    Enlarge

  33. pcDNA3.1_hHNF4alpha

    Plasmid
    #206878
    Purpose
    The ORF of HNF4α (variant 2) cDNA amplified from the RT human liver RNA was cloned at the Bam HI/XbaI sites of the pcDNA3.1(+), the mammalian expression vector.
    Depositor
    Catharine Ross, Reza Zolfaghari
    Article
    Zolfaghari et al J Cell Biochem. 2014 Oct;115(10):1740-51
    Insert
    Human hepatocyte nuclear factor 4 alpha (HNF4A Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    206878-plasmid-map-sequence-id-422375

    Enlarge

  34. pcDNA-dCas9-FLp300

    Plasmid
    #61356
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of FL human p300 (aa 2-2414) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal full length human p300 (aa 2-2414) (EP300 S. pyogenes, Human, Synthetic)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    CMV
    Available since
    April 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    61356-plasmid-map-sequence-id-99784

    Enlarge

  35. pCDNA3.1-nAChr-beta3-YFP

    Plasmid
    #50484
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Henry Lester
    Article
    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu
    Insert
    nAChRBeta3 subunit, isoform 1 (Chrnb3 Mouse)
    Use
    Tags
    YFP
    Expression
    Mammalian
    Mutation
    YFP fusion in M3-M4 loop (after residue P379). O…
    Promoter
    CMV
    Available since
    Jan. 13, 2014
    Availability
    Academic Institutions and Nonprofits only

  36. pUCmini-iCAP-AAV.CAP-B22

    Plasmid
    #175005
    Purpose
    non-standard AAV2 rep-AAV.CAP-B22 cap plasmid with AAV cap expression controlled by a tTA-TRE amplifcation system
    Depositor
    Viviana Gradinaru
    Article
    Goertsen et al Nat Neurosci. 2022 Jan;25(1):106-115. do
    Insert
    Synthetic construct isolate AAV.CAP-B22 VP1 gene
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    7 amino acid substitution between VP1 452 and VP1…
    Promoter
    p41
    Available since
    Nov. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    175005-plasmid-map-sequence-id-347133

    Enlarge

  38. pRSET-RCaMP1h

    Plasmid
    #42874
    Purpose
    a single-wavelength red-shifted genetically encoded calcium indicator constructed from calmodulin and cp-mRuby
    Depositor
    Loren Looger
    Article
    Akerboom et al Front Mol Neurosci. 2013;6:2. doi: 10.33
    Insert
    Red Fluorescent Calcium binding Protein
    Use
    Tags
    EK (Enterokinase) Recognition Site, His Tag (6x),…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 22, 2013
    Availability
    Academic Institutions and Nonprofits only
    42874-plasmid-map-sequence-id-58020

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Showing: 7241 - 7280 of 7585 results