We narrowed to 4,430 results for: Erf

1. spa-GCaMP6f

   Plasmid

   #67558

   Purpose

   photoactivatable fluorescent calcium reporter

   Depositor

   Ehud Isacoff

   Article

   Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

   Insert

   spa-GCaMP6f
   

   Use

   Tags

   HIS-tag

   Expression

   Mammalian

   Mutation

   K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

   Promoter

   CMV

   Available since

   July 28, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pAtCas9-dead_2

   Plasmid

   #91166

   Purpose

   Cas9 only expression, Cas9 Type: AtCas9_D10A+H840A (dead), Plant Selection: 2x35S:npt II

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   AtCas9_D10A+H840A (dead)
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   D10A, H840A

   Promoter

   Available since

   July 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. p.UTA.2.0-GL-2

   Plasmid

   #82440

   Purpose

   Dual Fluorescent endogenous miRNA sensor. Perfect complementary region of an siRNA control targeting firefly luciferase encoded by the pGL2

   Depositor

   Jens Gruber

   Article

   Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10

   Insert

   Luciferase
   

   Use

   Tags

   Expression

   Mutation

   The complementary region for GL2 siRNA control wa…

   Promoter

   Available since

   March 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. NAX_CAGGS_CAS9_m4_KRAB_EBFP2

   Plasmid

   #167873

   Purpose

   PiggyBac compatible plasmid expressing dCas9-KRAB

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9-KRAB
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A, D839A, H840A, N863A

   Promoter

   Available since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMOD_A0802

   Plasmid

   #91023

   Purpose

   Module A, Promoter: AtUbi10, Gene: Csy4-P2A-AtCas9_dead (D10A + H840A), Terminator: HSP

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Csy4-P2A-AtCas9_dead (D10A + H840A)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A, H840A

   Promoter

   AtUbi10

   Available since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28a-Hook3 aa 1-239

   Plasmid

   #74609

   Purpose

   Expresses Hook domain and first coiled coil of Hook3 in bacteria for purification

   Depositor

   Ron Vale

   Article

   Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

   Insert

   human Hook3 amino acids 1-239 (HOOK3 Human)
   

   Use

   Tags

   6x His, Strep II, and superfolder GFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAtCas9-dead_1

   Plasmid

   #91165

   Purpose

   Cas9 only expression, Cas9 Type: AtCas9_D10A+H840A (dead), Plant Selection: 2x35S:hpt II

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   AtCas9_D10A+H840A (dead)
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   D10A, H840A

   Promoter

   Available since

   July 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRSET-spa-GCaMP6f

   Plasmid

   #67563

   Purpose

   bacterial expression of a photoactivatable fluorescent calcium reporter

   Depositor

   Ehud Isacoff

   Article

   Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

   Insert

   spa-GCaMP6f
   

   Use

   Tags

   HIS-tag

   Expression

   Bacterial

   Mutation

   K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

   Promoter

   Available since

   July 29, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pLPC myc-mRAP1 I312R

   Plasmid

   #73137

   Purpose

   Expression of myc-tagged mRAP1 with single point mutatoin I312R

   Depositor

   Agnel Sfeir

   Article

   Yeung et al Cell Rep. 2013 Jun 27;3(6):1847-56. doi:

   Insert

   Rap1 (Terf2ip Mouse)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   isoleucine changed to an arginine at amino acid 3…

   Promoter

   Available since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pET28a-Hook3 aa 1-160

    Plasmid

    #74607

    Purpose

    Expresses Hook domain of Hook3 in bacteria for purification

    Depositor

    Ron Vale

    Article

    Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

    Insert

    human Hook3 amino acids 1-160 (HOOK3 Human)
    

    Use

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAtCas9-dead_3

    Plasmid

    #91167

    Purpose

    Cas9 only expression, Cas9 Type: AtCas9_D10A+H840A (dead), Plant Selection: 2x35S:bar

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    AtCas9_D10A+H840A (dead)
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A, H840A

    Promoter

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. spa-GCaMP6m

    Plasmid

    #67557

    Purpose

    photoactivatable fluorescent calcium reporter

    Depositor

    Ehud Isacoff

    Article

    Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

    Insert

    spa-GCaMP6m
    

    Use

    Tags

    HIS-tag

    Expression

    Mammalian

    Mutation

    K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

    Promoter

    Available since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pMOD_A1410

    Plasmid

    #91035

    Purpose

    Module A, Promoter: ZmUbi, Gene: TaCas9_dead (D10A + H840A), Terminator: HSP

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    TaCas9_dead (D10A + H840A)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, H840A

    Promoter

    ZmUbi

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. p.UTA.2.0 miR 19b-3p

    Plasmid

    #82442

    Purpose

    Dual Fluorescent endogenous miRNA sensor. Perfect complementary region of hsa-miR-19b-3p

    Depositor

    Jens Gruber

    Article

    Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10

    Insert

    hsa-miR-19-3p
    

    Use

    Tags

    Expression

    Mutation

    Complementary region for hsa-miR-19-3p as sensor

    Promoter

    Available since

    March 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. sspa-GCaMP6m

    Plasmid

    #67560

    Purpose

    short photoactivatable fluorescent calcium reporter

    Depositor

    Ehud Isacoff

    Article

    Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

    Insert

    sspa-GCaMP6m
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

    Promoter

    Available since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pCMV-MMLVgag-dCas9-KOX1

    Plasmid

    #240528

    Purpose

    Expresses MMLVgag–dCas9-KOX1 for producing RENDER-dCas9-KOX1

    Depositor

    James Nuñez

    Article

    Xu et al Nat Commun. 2025 Aug 26;16(1):7948. doi:

    Insert

    MMLVgag-dCas9-KOX1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pmEos3.1-N1 GBP

    Plasmid

    #220492

    Purpose

    Expression in mammalian cells of GFP binding protein (GBP) tagged with photoconvertable protein mEos3.1 to track GFP proteins of interest to perform Fluorescent intrabody Localization Microscopy

    Depositor

    Frederic Meunier

    Article

    Gormal et al Nat Protoc. 2025 Aug 8. doi: 10.1038/s41

    Insert

    GFP Binding Protein tagged with mEos3.1
    

    Use

    Tags

    mEos3.1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. IRF3-V5

    Plasmid

    #169775

    Purpose

    Express IRF3 in mammalian cells

    Depositor

    Sung-Liang Yu

    Article

    Chang et al Commun Biol. 2021 Jun 2;4(1):663. doi: 1

    Insert

    Homo sapiens interferon regulatory factor 3 (IRF3), transcript variant 1 (IRF3 Human)
    

    Use

    Tags

    V5 tag

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    July 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSEVAb827

    Plasmid

    #217500

    Purpose

    pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb82 backbone, RK2 ori

    Depositor

    Nico Claassens

    Article

    van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

    Insert

    superfolder green fluorescent protein (sfGFP)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

    Promoter

    Available since

    July 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSEVAb837

    Plasmid

    #217501

    Purpose

    pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb83 backbone, pBBR1 ori

    Depositor

    Nico Claassens

    Article

    van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

    Insert

    superfolder green fluorescent protein (sfGFP)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

    Promoter

    Available since

    July 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge