We narrowed to 4,430 results for: Erf

1. pSEVAb847

   Plasmid

   #217502

   Purpose

   pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb84 backbone, pRO1600/ColE1 ori

   Depositor

   Nico Claassens

   Article

   van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

   Insert

   superfolder green fluorescent protein (sfGFP)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

   Promoter

   Available since

   July 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSEVAb867

   Plasmid

   #217503

   Purpose

   pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb86 backbone, p15A ori

   Depositor

   Nico Claassens

   Article

   van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

   Insert

   superfolder green fluorescent protein (sfGFP)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

   Promoter

   Available since

   July 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSEVAb887

   Plasmid

   #217504

   Purpose

   pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb88 backbone, pUC ori

   Depositor

   Nico Claassens

   Article

   van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

   Insert

   superfolder green fluorescent protein (sfGFP)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

   Promoter

   Available since

   July 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSEVAb897

   Plasmid

   #217505

   Purpose

   pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb89 backbone, pBR322/ROP ori

   Depositor

   Nico Claassens

   Article

   van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

   Insert

   superfolder green fluorescent protein (sfGFP)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

   Promoter

   Available since

   July 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pENTR_sfGFP-TurboID

   Plasmid

   #240226

   Purpose

   Gateway entry clone with TurboID tagged with superfolder GFP (contains stop codon)

   Depositor

   Justin Bosch, Norbert Perrimon

   Article

   Bosch et al bioRxiv 2025.07.09.659702; doi: https://

   Insert

   sfGFP-TurboID
   

   Use

   Gateway shuttle vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLD-puro-Cc-IFIT5_K206R-VA

   Plasmid

   #191224

   Purpose

   Lentiviral expression of human IFIT5_K206R in mammalian cells

   Depositor

   Mohan Babu

   Insert

   interferon-induced protein with tetratricopeptide repeats 5 (IFIT5 Human)
   

   Use

   Lentiviral

   Tags

   3xFlag-TEVx2-6xHis-Strep x2 tag and VA tag

   Expression

   Mammalian

   Mutation

   changed Lysine 206 to Arginine (K206R)

   Promoter

   CMV

   Available since

   April 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-hfYFP-TEV-SAV

   Plasmid

   #191197

   Purpose

   Fusion of hfYFP and Streptavidin core domain separated by a TEV cleavage site for fluorescence-assisted protein purification

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   hfYFP & Streptavidin fusion
   

   Use

   Tags

   6xHis and Streptavidin core domain, amino acids 1…

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-dCas9-FCPF

   Plasmid

   #220133

   Purpose

   Expresses dCas9-FCPF in E.coli

   Depositor

   Xinlai Cheng

   Article

   Altinbay et al Nucleic Acids Res. 2024 Sep 24:gkae798.

   Insert

   dCas9-FCPF (cas9 S. pyogenes, Synthetic)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   FCPF sequence inserted on C-terminal

   Promoter

   T7

   Available since

   Oct. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. RNF130 shRNA

   Plasmid

   #225340

   Purpose

   shRNAmir backbone for RNA interference, with YFP

   Depositor

   Huda Zoghbi

   Article

   Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

   Insert

   RNF130 shRNA (Rnf130 Mouse)
   

   Use

   AAV and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CBA

   Available since

   Sept. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTSAR3.6t

    Plasmid

    #210258

    Purpose

    Fluorescent reporter for Type III secretion system activity in Shigella flexneri

    Depositor

    François-Xavier Campbell-Valois

    Article

    Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

    Inserts

    mScarlet
    superfolder GFP
    

    Use

    Tags

    ssra

    Expression

    Bacterial

    Mutation

    Promoter

    ipaH7.8p and rpsM

    Available since

    April 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTSAR3.7t

    Plasmid

    #210259

    Purpose

    Fluorescent reporter for Type III secretion system activity in Shigella flexneri

    Depositor

    François-Xavier Campbell-Valois

    Article

    Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

    Inserts

    e2Crimson
    superfolder GFP
    

    Use

    Tags

    ssra

    Expression

    Bacterial

    Mutation

    Promoter

    ipaH7.8p and rpsM

    Available since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTSAR3.3t

    Plasmid

    #210255

    Purpose

    Fluorescent reporter for Type III secretion system activity in Shigella flexneri

    Depositor

    François-Xavier Campbell-Valois

    Article

    Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

    Inserts

    DsRed
    superfolder GFP
    

    Use

    Tags

    ssra

    Expression

    Bacterial

    Mutation

    Promoter

    ipaH7.8p and rpsM

    Available since

    March 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. GL4.10 Prg4 promoter-luciferase

    Plasmid

    #195034

    Purpose

    Prg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vector

    Depositor

    Cheng-Hai Zhang, Andrew Lassar

    Article

    Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

    Insert

    Prg4 promoter (PRG4 Bovine)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDN0617 (pDEST-mEGFP C-term, NatR)

    Plasmid

    #210484

    Purpose

    pDEST vector to tag gene of interest with mEGFP on the C-terminus to perform localization assays in S. cerevisiae.

    Depositor

    Christian Landry

    Article

    Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology; Destination vector for gateway…

    Tags

    mEGFP

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pQE60 M66L wgMDH

    Plasmid

    #204247

    Purpose

    Bacterial expression of M66L wgMDH with IPTG induction - Subunit interface mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    M66L

    Promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pQE60 M91Q wgMDH

    Plasmid

    #204250

    Purpose

    Bacterial expression of M91Q wgMDH with IPTG induction - Subunit interface mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    M91Q

    Promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pQE60 D87L wgMDH

    Plasmid

    #204248

    Purpose

    Bacterial expression of D87L wgMDH with IPTG induction - Subunit interface mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    D87L

    Promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. NAX_CAGGS_CAS9_m4_KRAB-MeCP2_Hygro

    Plasmid

    #167899

    Purpose

    PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Sept. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSLiP-G2

    Plasmid

    #188964

    Purpose

    L-Rhamnose inducible catalytically inactive Cas9 with sgRNA for bacterial gene knockdown

    Depositor

    Seung-Goo Lee

    Article

    Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

    Inserts

    dCas9
    sgRNA: gttagacgctgattacatggactagg
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PrhaBAD

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. NAX_CAGGS_CAS9_m4_KRAB-MeCP2_EBFP2

    Plasmid

    #167897

    Purpose

    PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only