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Plasma membrane-targeted optodPLD

Plasmid

#140061

Purpose

Encodes CRY2-mCherry-dPLD-P2A-CIBN-CAAX for mammalian expression

Depositor

Jeremy Baskin

Article

Tei et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Phospholipase D

Tags

CAAX, CIBN, CRY2, P2A, and mCherry

Expression

Mammalian

Mutation

H170A (catalytic dead)

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pET28_mouse-pro-IL18

Plasmid

#183389

Purpose

Overexpression of catalytic domain of inflammatory caspase in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

pro-IL18 (Il18 Mouse)

Tags

His-TEV

Expression

Bacterial

Promoter

T7

Available Since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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pAM552

Plasmid

#154131

Purpose

Expresses wt rRNAs operon in bacterial cells

Depositor

Alexander Mankin

Article

Huang et al Nat Commun. 2020 Jun 9;11(1):2900. doi:

Insert

wt bacterial rRNAs operon under control of pL promoter

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pFastBac_human-IL-18

Plasmid

#214338

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (IL18 Human)

Tags

His-TEV

Expression

Bacterial

Promoter

T7

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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His6-PRC1

Plasmid

#69111

Purpose

For bacterial expression of recombinant human PRC1 tagged with His.

Depositor

Stephen Royle

Article

Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife

Insert

Protein Regulator of Cytokinesis 1 (PRC1 Human)

Tags

His6

Expression

Bacterial

Available Since

Sept. 24, 2015

Availability

Academic Institutions and Nonprofits only

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HA.PKD.K/W

Plasmid

#10809

Depositor

Alex Toker

Article

Storz et al J Biol Chem. 2003 May 16. 278(20):17969-

Insert

PKD (PRKD1 Human)

Tags

HA and Myc (inserted between amino acids 33&3…

Expression

Mammalian

Mutation

Kinase dead mutant: K612W (Lys 612 to Trp) (bp183…

Promoter

CMV

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only
pCMV5B-TFGbeta receptor II K277R

Plasmid

#11762

Depositor

Joan Massague, Jeff Wrana

Article

Wrana et al Cell. 1992 Dec 11. 71(6):1003-14.

Insert

TGFbeta receptor II (TGFBR2 Human)

Tags

His

Expression

Mammalian

Mutation

K277R: kinase dead

Promoter

CMV

Available Since

May 12, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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human PNP non-tagged

Plasmid

#64199

Purpose

Expression of native human PNP (non-tagged)

Depositor

Vern Schramm

Article

Murkin et al Biochemistry. 2007 May 1;46(17):5038-49.

Insert

Purine Nucleoside Phosphorylase (PNP Human)

Tags

none

Expression

Bacterial

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-C2gamma

Plasmid

#21215

Depositor

Tobias Meyer

Article

Codazzi et al Curr Biol. 2001 Jul 24. 11(14):1089-97.

Insert

GFP-pcDNA3-PKCgamma-c2 (Prkcg Rat)

Tags

GFP

Expression

Mammalian

Mutation

The C2-domain from PKCgamma (aa170-260) plus flan…

Available Since

July 13, 2009

Availability

Academic Institutions and Nonprofits only

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PT7-deGFP-MGapt

Plasmid

#67741

Purpose

T7 promoter expressing deGFP-MG aptamer fusion

Depositor

Richard Murray

Article

Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

Insert

UTR1-deGFP-MGapt-T7term

Use

Synthetic Biology

Expression

Bacterial

Promoter

PT7

Available Since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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GLI K12

Plasmid

#16419

Depositor

Bert Vogelstein

Article

Kinzler et al Nature. 1988 Mar 24. 332(6162):371-4.

Insert

GLI (GLI1 Human)

Expression

Bacterial

Available Since

March 10, 2008

Availability

Academic Institutions and Nonprofits only

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Cdk2

Plasmid

#1883

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

Cdk2 (CDK2 Human)

Expression

Mammalian

Available Since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only
pT2ADWpuro_2paRAF

Plasmid

#129653

Purpose

For generating cell line expressing the 2P-activatable RAF system with P2A and full-length RAF1

Depositor

Michiyuki Matsuda

Article

Kinjo et al Nat Methods. 2019 Sep 9. pii: 10.1038/s4

Insert

2paCRY2-Raf1-2A-CIBN-mScarlet-KRasCAAX

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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AMPKA2

Plasmid

#36194

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

AMPKA2 (PRKAA2 Human)

Tags

His tag with thrombin cleavage

Expression

Bacterial

Mutation

contains KINASE DOMAIN only

Available Since

Aug. 8, 2012

Availability

Industry, Academic Institutions, and Nonprofits
pKM808

Plasmid

#134181

Purpose

Lentiviral sgRNA plasmid with blasticidin resistance

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 WAVE 2

Plasmid

#67098

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

WAVE 2 (WASF2 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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PCTAIRE-1-HA

Plasmid

#1999

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

PCTAIRE-1 (CDK16 Human)

Tags

HA

Expression

Mammalian

Available Since

Jan. 24, 2006

Availability

Academic Institutions and Nonprofits only
SLBP non-targeting gRNA lambda phage

Plasmid

#132548

Purpose

expresses gRNA for SLBP-based CIRTS

Depositor

Bryan Dickinson

Article

Rauch et al Cell. 2019 Jun 27;178(1):122-134.e12. do

Insert

gRNA SLBP-based CIRTS

Expression

Mammalian

Promoter

hU6 promoter

Available Since

Sept. 23, 2019

Availability

Academic Institutions and Nonprofits only

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Cdk2-DN

Plasmid

#1882

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

Cdk2 (CDK2 Human)

Expression

Mammalian

Mutation

D146N, dominant negative

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
Cdk5

Plasmid

#1870

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

Cdk5 (CDK5 Human)

Expression

Mammalian

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only