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pcDNA3-hsGFP

Plasmid

#64912

Purpose

Encodes a Fluorescent Reporter for Hydrogen Sulfide in Mammalian Cells

Depositor

Huiwang Ai

Article

Chen et al Biochemistry. 2014 Sep 23;53(37):5966-74

Insert

hsGFP

Tags

6xHis

Expression

Mammalian

Mutation

Chromophore tyrosine of the protein mutated to TA…

Promoter

CMV

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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pMM575

Plasmid

#68881

Purpose

NanoLuc expressed constiutively from PcfiA, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Insert

NanoLuc

Expression

Bacterial

Available Since

Oct. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pC1-HyPer-C199S

Plasmid

#42213

Purpose

Peroxide-insensitive version of HyPer to be used as a pH-control. Can be applied as a pH sensor.

Depositor

Vsevolod Belousov

Article

Belousov et al Nat Methods. 2006 Apr;3(4):281-6.

Insert

HYPER C199S

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pHR-TcRb:mCherry-FKBP

Plasmid

#72904

Purpose

ER-targeted mCherry-tagged recruiter construct

Depositor

Ivan Yudushkin

Article

Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

Insert

T cell receptor β chain

Use

Lentiviral

Tags

biotinylation acceptor peptide (AP) and mCherry, …

Expression

Mammalian

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pBH504

Plasmid

#215782

Purpose

NBU1 backbone with constitutive dCas9, LacI(YQR), P(O1)_sgRNA targeting B. theta amylopectin SusC gene

Depositor

Corey Wilson

Article

Huang et al Nat Commun. 2022 Jul 6;13(1):3901. doi:

Inserts

LacI(YQR)
sgRNA-ribozyme cassette
dCas9

Use

Synthetic Biology

Expression

Bacterial

Promoter

Bt1311, P(O1), and P1

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pMM597

Plasmid

#68886

Purpose

IPTG-inducible NanoLuc expression, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Inserts

NanoLuc
LacI

Expression

Bacterial

Available Since

Oct. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pAc5.1-PH-mCherry (FspAI) (HK49)

Plasmid

#206453

Purpose

Expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Type

Empty backbone

Expression

Insect

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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NM R2E2 GFP

Plasmid

#1208

Depositor

Susan Lindquist

Article

Liu et al Nature 1999 Aug 5;400(6744):573-6.

Insert

sup35 NM domain (SUP35 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

2 additional copies of second oligopeptide repeat…

Available Since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

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pMM659

Plasmid

#68888

Purpose

Chondroitin sulfate-inducible NanoLuc expression, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Insert

NanoLuc

Expression

Bacterial

Available Since

Oct. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pAT593

Plasmid

#68909

Purpose

NanoLuc expressed constiutively from PBT1311 and rpiL* RBS, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Insert

NanoLuc

Expression

Bacterial

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-mCherry(-6)

Plasmid

#205045

Purpose

Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

Depositor

Allie Obermeyer

Article

Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

Insert

mCherry(-6)

Tags

MGHHHHHGG

Expression

Bacterial

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pAT588

Plasmid

#68907

Purpose

NanoLuc expressed constiutively from PBT1311 and GH023 RBS, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Insert

NanoLuc

Expression

Bacterial

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pNano

Plasmid

#169895

Purpose

Minimal cloning vector

Depositor

Richard Schneider

Article

Chu et al Biol Open. 2020 Oct 6;9(10). pii: bio.05

Type

Empty backbone

Use

Cloning

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCI 9.0

Plasmid

#74229

Purpose

Luminescent coincidence reporter with FLAG tags, FLuc-P2A-NLucPEST

Depositor

James Inglese

Article

Schuck et al Curr Protoc Neurosci. 2017 Apr 10;79:5.3

Insert

3xFlag-FLuc-P2A-3xFlag-NLucPEST

Expression

Mammalian

Promoter

None

Available Since

May 18, 2017

Availability

Academic Institutions and Nonprofits only

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P-BT3332

Plasmid

#188110

Purpose

pBolux harboring the promoter region upstream of BT3332

Depositor

Guy Townsend

Article

Modesto et al Nat Commun. 2023 Jan 17;14(1):275. doi:

Insert

The B. thetaiotaomicron BT3332 promoter cloned into pBolux

Expression

Bacterial

Promoter

BT3332

Available Since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pMM585

Plasmid

#68884

Purpose

IPTG-inducible NanoLuc expression, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Inserts

NanoLuc
LacI

Expression

Bacterial

Available Since

Oct. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pCI 9.4

Plasmid

#74230

Purpose

Luminescent coincidence reporter with FLAG tags, FLuc-P2A-NLuc

Depositor

James Inglese

Article

Schuck et al Curr Protoc Neurosci. 2017 Apr 10;79:5.3

Insert

3xFlag-FLuc-P2A-3xFlag-NLuc

Expression

Mammalian

Available Since

May 18, 2017

Availability

Academic Institutions and Nonprofits only

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pMM555

Plasmid

#68880

Purpose

NanoLuc expressed constiutively from PcfxA, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Insert

NanoLuc

Expression

Bacterial

Available Since

Oct. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pAT937

Plasmid

#68936

Purpose

Rhamnose-inducible Int12 expression vector with rpiL*C51 RBS, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Insert

Int12

Expression

Bacterial

Available Since

Oct. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pAT892

Plasmid

#68933

Purpose

Int9 expressed constitutively from PAM4 and the rpiL* RBS, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Insert

Int9

Expression

Bacterial

Available Since

Oct. 2, 2015

Availability

Academic Institutions and Nonprofits only

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