We narrowed to 20,302 results for: fus
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Plasmid#72346PurposeMammalian expression of cytoplasmic mMBP-fused proteins with C-terminal 6His-tag/Strep-tag II/HA-tagDepositorTypeEmpty backboneTags6His-tag, HA-tag, Strep-tag II, and mMBPExpressionMammalianPromoterCMV enhancer + chicken beta-actin promoterAvailable SinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only
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pAAVS1-TRE-MA4-GFP
Plasmid#114380PurposeDoxycycline inducible MLL-AF4 and GFP expression. The construct has been used with CAG-rtTA for making engraftable iPSC derived HSCs.DepositorAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMK349 (BSD-P2A-mAID-mCherry)
Plasmid#121183PurposeN-terminal taggingDepositorInsertBSD-P2A-mAID-mCherry
UseCRISPRAvailable SinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSLGCV
Plasmid#49862PurposeExpression in Caenorhabditis elegans of the luc+ gene (Promega), fused in frame to GFP (S65C), under the sur-5 promoter (from plasmid pTG96, John Yochem and Min Han). Backbone pPD95.79 (Firelab).DepositorInsertfirefly luciferase luc+ fused to GFP (S65C)
TagsGFP (S65C)ExpressionWormPromotersur-5Available SinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBS-slpGFP
Plasmid#27171DepositorInsertsurface (S)-layer protein promoter region fused with modified green fluorescent protein 5
ExpressionBacterialAvailable SinceJan. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pJFA334E9
Plasmid#49237PurposeTET1-catalytic domain (CD) expression vector for TALE-TET1 fusion protein to induce targeted demethylation in mammalian cellsDepositorInsertTET1 tet methylcytosine dioxygenase 1 (TET1 Human, Synthetic)
UseTALEN; Tale-tet1 expression vectorPromoterEF1aAvailable SinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJFA334E9
Plasmid#49237PurposeTET1-catalytic domain (CD) expression vector for TALE-TET1 fusion protein to induce targeted demethylation in mammalian cellsDepositorInsertTET1 tet methylcytosine dioxygenase 1 (TET1 Human, Synthetic)
UseTALEN; Tale-tet1 expression vectorPromoterEF1aAvailable SinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSLIK-NFLAG-hEWSR1P552L-CMyc
Plasmid#50736PurposeProduces lentivirus expressing human EWSR1P552L mutant with FLAG tag at its N-terminus and Myc tag at its C-terminus by co-transfection with psPAX2 and pMD2GDepositorInsertEWSR1 P552L (EWSR1 Human)
UseLentiviralTagsFLAG and MycMutationChanged Pro 552 to LeuPromoterTREAvailable SinceJan. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
MSCVneo-HA-ER-Hoxb8
Plasmid#222291PurposeThe most commonly used plasmid for the generation of ER-Hoxb8 conditionally immortalized myeloid cell lines.DepositorUseRetroviralTagsHAExpressionMammalianMutationThe estrogen receptor hormone binding domain cont…Available SinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYPQ154
Plasmid#69305PurposeGateway entry vector with AteCas9 (Arabidopsis codon optimized)DepositorInsertAteCas9 (Arabidopsis codon optimized)
UseCRISPRMutationNLS fusionAvailable SinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
MSCVneo-HA-ER-Hoxb8
Plasmid#222291PurposeThe most commonly used plasmid for the generation of ER-Hoxb8 conditionally immortalized myeloid cell lines.DepositorUseRetroviralTagsHAExpressionMammalianMutationThe estrogen receptor hormone binding domain cont…Available SinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYPQ154
Plasmid#69305PurposeGateway entry vector with AteCas9 (Arabidopsis codon optimized)DepositorInsertAteCas9 (Arabidopsis codon optimized)
UseCRISPRMutationNLS fusionAvailable SinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUASTattB_NSlmb-vhhGFP4
Plasmid#35577DepositorInsertNSlmb-vhhGFP4 (slmb Fly)
ExpressionInsectAvailable SinceApril 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUASTattB_NSlmb-vhhGFP4
Plasmid#35577DepositorInsertNSlmb-vhhGFP4 (slmb Fly)
ExpressionInsectAvailable SinceApril 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
EF1a-dCasRx-RBM25
Plasmid#221001PurposeTransient expression of dCasRx-RBM25. EF1a promoter.DepositorInsertdCasRx-RBM25
UseCRISPRTagsNLS and NLS-HAExpressionMammalianPromoterEF1aAvailable SinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Axin-IRES-GFP
Plasmid#14716DepositorAvailable SinceAug. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-DNMT3A-PuroR (ANV)_v2
Plasmid#74408PurposeExpression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR (v2); cloning backbone for sgRNADepositorInsertS. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (v2) (DNMT3A Human, Synthetic, S. pyogenes)
UseCRISPRTags3xFLAG, SV40 NLS, and T2A-PuroRExpressionMammalianMutationD10A and H840A in S.pyogenes Cas9; E756A inactiva…PromoterCBhAvailable SinceApril 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
EF1a-dCasRx-RBM25
Plasmid#221001PurposeTransient expression of dCasRx-RBM25. EF1a promoter.DepositorInsertdCasRx-RBM25
UseCRISPRTagsNLS and NLS-HAExpressionMammalianPromoterEF1aAvailable SinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Axin-IRES-GFP
Plasmid#14716DepositorAvailable SinceAug. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
Lenti-TetON-Sox2
Plasmid#110280PurposeDoxycycline-inducible overexpression of Sox2DepositorInsertSRY (sex determining region Y)-box 2 (Sox2 Mouse)
UseLentiviralTagsNo fusion genesExpressionMammalianMutationWild typePromoterTREAvailable SinceOct. 16, 2018AvailabilityAcademic Institutions and Nonprofits only