We narrowed to 22,823 results for: Sis

1. hSUMO1 GFP

   Plasmid

   #118360

   Purpose

   This plasmid expresses the full length human SUMO1.

   Depositor

   Lea Sistonen

   Article

   Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

   Insert

   hSUMO1 (SUMO1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available Since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. R4pGWB601_UBQ10p-miniTurbo-YFP-NLS

   Plasmid

   #127370

   Purpose

   Binary vector for expressing nuclear miniTurbo-YFP under the UBQ10 promoter in plants

   Depositor

   Dominique Bergmann

   Article

   Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

   Insert

   miniTurbo (BirA mutant)
   

   Use

   Tags

   GS linker, NLS, V5, and YFP

   Expression

   Plant

   Mutation

   aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

   Promoter

   Arabidopsis UBQ10 promoter

   Available Since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT7C-HSP90AA1

   Plasmid

   #177658

   Purpose

   Recombinant protein synthesis of human HSP90AA1

   Depositor

   Jeff Kuret

   Article

   Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

   Insert

   HSP90AA1 (HSP90AA1 Human)
   

   Use

   Tags

   6xHis-Thrombin

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-FLEX-SaCas9-U6-sgGrin2b

   Plasmid

   #124851

   Purpose

   Mutagenesis of Grin2b

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Grin2b (Grin2b Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCdOpt-BMX

   Plasmid

   #203929

   Purpose

   This plasmid contains a codon optimized BleMX drug resistance cassette for generating gene deletions in C. glabrata, C. auris, C. albicans or S. cerevisiae

   Depositor

   Scott Briggs

   Article

   Gregor et al mSphere. 2023 Nov 6:e0031123. doi: 10.11

   Insert

   BleMX
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   BleMX was codon optimized for Candida albicans or…

   Promoter

   Available Since

   Dec. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFB2G

   Plasmid

   #195290

   Purpose

   Bicistronic HSV-TK and mTagBFP resistance for positive/negative selection

   Depositor

   Scott Lowe

   Article

   Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

   Insert

   HSV-TK 2A mTagBFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available Since

   Jan. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. aMHC-mCherry-Rex-Blasticidin

   Plasmid

   #21228

   Depositor

   Mark Mercola

   Article

   Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

   Insert

   alpha myosin heavy chain promoter
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Available Since

   June 30, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSH-EFIRES-B-AtAFB2-mCherry

   Plasmid

   #129718

   Purpose

   AAVS1 safe harbor targeting vector with blasticidin resistance gene expressing AtAFB2-mCherry

   Depositor

   Elina Ikonen

   Article

   Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

   Insert

   AFB2 (AFB2 Mustard Weed)
   

   Use

   CRISPR and TALEN; Human safe harbor locus (a avs1…

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   EF1a

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pENTR4 CDS A. thaliana PHOT1

   Plasmid

   #209187

   Purpose

   pENTR4 plasmid with CDS of Arabidopsis thaliana PHOTOTROPIN 1 (PHOT1, AT3G45780.1) without stop codon for C-terminal epitope tags

   Depositor

   Lennart Wirthmueller

   Article

   Simranjit et al bioRxiv 2023.09.25.558804

   Insert

   AT3G45780.1 (PHOT1 Mustard Weed)
   

   Use

   Gateway-compatible entry vector

   Tags

   Expression

   Mutation

   Promoter

   none

   Available Since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. CD8-mCherry-FRB

    Plasmid

    #100738

    Purpose

    To express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    CD8A (CD8A Human)
    

    Use

    Tags

    FRB and mCherry

    Expression

    Mammalian

    Mutation

    Truncated

    Promoter

    CMV

    Available Since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. FKBP-epsin(144-575)-GFP

    Plasmid

    #100733

    Purpose

    To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    epn1 (Epn1 Mouse)
    

    Use

    Tags

    FKBP and GFP

    Expression

    Mammalian

    Mutation

    clathrin binding middle part aa 144-575

    Promoter

    CMV

    Available Since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMV306DIG13+FFlucRT

    Plasmid

    #49998

    Purpose

    Integrase removed to increase stability of the plasmid in mycobacteria

    Depositor

    Brian Robertson

    Article

    Andreu et al J Antimicrob Chemother. 2013 Sep;68(9):2

    Insert

    G13 promoter + Firefly luciferase Red Thermostable
    

    Use

    Mycobacteria integrating vector

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for Mycobacterium tuberculosis wi…

    Promoter

    G13

    Available Since

    May 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. R4pGWB601_UBQ10p-miniTurbo-NES-YFP

    Plasmid

    #127369

    Purpose

    Binary vector for expressing cytosolic miniTurbo-YFP under the UBQ10 promoter in plants

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    miniTurbo (BirA mutant)
    

    Use

    Tags

    GS linker, NES, V5, and YFP

    Expression

    Plant

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    Arabidopsis UBQ10 promoter

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. aMHC-eGFP-Rex-Neo

    Plasmid

    #21229

    Depositor

    Mark Mercola

    Article

    Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

    Insert

    alpha myosin heavy chain promoter
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    Available Since

    June 30, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMV306G13+FFlucRT

    Plasmid

    #49997

    Purpose

    Integrating vector with an improved Red-shifted Thermostable Firefly Luciferase

    Depositor

    Brian Robertson

    Article

    Andreu et al J Antimicrob Chemother. 2013 Sep;68(9):2

    Insert

    G13 promoter + Firefly luciferase Red Thermostable
    

    Use

    Mycobacteria integrating vector

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for Mycobacterium tuberculosis wi…

    Promoter

    G13 from M. marinum

    Available Since

    May 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-FLEX-SaCas9-U6-sgGabrg2

    Plasmid

    #124857

    Purpose

    Mutagenesis of Gabrg2

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Gabrg2 (Gabrg2 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSUPER-hHSF1 shRNA

    Plasmid

    #118345

    Purpose

    This plasmid expresses shRNA against hHSF1.

    Depositor

    Lea Sistonen

    Article

    Ostling et al J Biol Chem. 2007 Mar 9;282(10):7077-86.

    Insert

    hHSF1 shRNA (HSF1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available Since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. Lenti-sgRNA-Cre-GpNLuc

    Plasmid

    #208382

    Purpose

    Derived from LentiCRISPRV2 by replacing Cas9 with Cre recombinase and puromycin resistance with GpNLuc. Contains a stuffer sequence for cloning of sgRNA of interest, as per LentiCRISPRV2.

    Depositor

    Gaorav Gupta

    Article

    Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

    Type

    Empty backbone

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available Since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMax_mPlum_C207G/T208C

    Plasmid

    #177831

    Purpose

    Site-specific mutagenesis of mPlum for the purposes of creating a site-specific O6-MeG lesion for the purposes of measuring MGMT activity via Host Cell Reactivation (HCR).

    Depositor

    Leona Samson, Zachary Nagel

    Article

    Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

    Insert

    mPlum_C207G/T208C
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C207G + T208C

    Promoter

    CMV

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBW_0065

    Plasmid

    #102817

    Purpose

    pVec8 containing Sr22 gene

    Depositor

    Brande Wulff

    Article

    Md Hatta et al Mol Plant Microbe Interact. 2020 Nov;33(

    Insert

    wheat stem rust resistance gene Sr22 (NEWENTRY Synthetic)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Native

    Available Since

    Feb. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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