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AiP12514 - pAAV-3xSP10ins-AiE0367h_C2-minRho-SYFP2-WPRE3-BGHpA (Alias: CN2514)

Plasmid

#191717

Purpose

AI plasmid ALIAS: AiP12514. Expression of SYFP2 (yellow fluorescent protein) in striatal medium spiny neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2

Use

AAV

Mutation

none

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pSpliceExpress

Plasmid

#32485

Depositor

Stefan Stamm

Article

Kishore et al Gene. 2008 Dec 31;427(1-2):104-10. Epub

Type

Empty backbone

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pDESTsplice

Plasmid

#32484

Depositor

Stefan Stamm

Article

Kishore et al Gene. 2008 Dec 31;427(1-2):104-10. Epub

Type

Empty backbone

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1a-eFFly-mCherry

Plasmid

#104833

Purpose

3rd generation lentiviral vector. eFFly luciferase for in vivo Imaging, mCherry for fluorescent detection

Depositor

Irmela Jeremias

Article

Ebinger et al Cancer Cell. 2016 Dec 12;30(6):849-862.

Inserts

eFFly
mCherry

Use

Lentiviral and Luciferase

Expression

Mammalian

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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hKCNQ1 pCDNA3.1(-)

Plasmid

#219953

Purpose

Expresses human KCNQ1 in mammalian cells

Depositor

Niels Decher

Article

Kiper et al Proc Natl Acad Sci U S A. 2024 Jun 18;12

Insert

KCNQ1 (KCNQ1 Human)

Expression

Mammalian

Mutation

contains silent sequence variations

Promoter

CMV

Available Since

Aug. 13, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pOG44_FLP_recombinase (pEHA1338)

Plasmid

#209087

Purpose

FLP recombinase for FRT-mediated integration

Depositor

Vikram Khurana

Article

Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

Insert

FLP recombinase

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO (pEHA1339)

Plasmid

#209088

Purpose

Backbone plasmid for Tet Responsive transgene expression, FRT integration

Depositor

Vikram Khurana

Article

Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pEF6-Acly

Plasmid

#70765

Purpose

Expresses mouse ATP-citrate lyase in mammalian cells

Depositor

Kathryn Wellen

Article

Lee et al Cell Metab. 2014 Aug 5;20(2):306-19. doi

Insert

ATP Citrate Lyase (Acly Mouse)

Tags

myc-epitope

Expression

Mammalian

Promoter

EF1alpha

Available Since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only

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CRISPR-SB

Plasmid

#177936

Purpose

Expresses SpCas9 and sgRNA. Can be integrated into the genome upon co-delivery of Sleeping Beauty transposase.

Depositor

Roland Rad

Article

Kaltenbacher et al Nat Protoc. 2022 Apr;17(4):1142-1188. do

Type

Empty backbone

Use

CRISPR; Transposon

Expression

Mammalian

Promoter

U6

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti‐Cas9‐2A‐Blast

Plasmid

#73310

Purpose

Lentiviral vector expressing human codon-optimized S. pyogenes FLAG-tagged Cas9 and blasticidin resistance from EFS promoter

Depositor

Jason Moffat

Article

Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

Insert

Cas9

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EFS-NS

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pEF6-Acly(S455D)

Plasmid

#70768

Purpose

Expresses phosphomimetic mouse ATP-citrate lyase in mammalian cells

Depositor

Kathryn Wellen

Article

Lee et al Cell Metab. 2014 Aug 5;20(2):306-19. doi

Insert

ATP Citrate Lyase (Acly Mouse)

Tags

myc-epitope

Expression

Mammalian

Mutation

S455D

Promoter

EF1alpha

Available Since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pEF6-Acly(S455A)

Plasmid

#70767

Purpose

Expresses phosphomutant mouse ATP-citrate lyase in mammalian cells

Depositor

Kathryn Wellen

Article

Lee et al Cell Metab. 2014 Aug 5;20(2):306-19. doi

Insert

ATP Citrate Lyase (Acly Mouse)

Tags

myc-epitope

Expression

Mammalian

Mutation

S455A

Promoter

EF1alpha

Available Since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only

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CS-TRE-c-Myc-Ubc-rtTA

Plasmid

#198190

Purpose

Generation of Expandable megakaryocyte cell lines from human iPS cells

Depositor

Hiromitsu Nakauchi

Article

Nakamura et al Cell Stem Cell. 2014 Apr 3;14(4):535-48.

Insert

hC-MYC (MYC Human)

Use

Lentiviral

Expression

Mammalian

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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iBE4max YE1 NG CLYBL

Plasmid

#174570

Purpose

doxycycline inducible BE4max YE1 NG expression for human CLYBL locus knock-in

Depositor

Mathew Garnett

Article

Coelho et al Cancer Cell. 2023 Feb 13;41(2):288-303.e

Insert

BE3

Expression

Mammalian

Promoter

tet ON

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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hV1aR-GFP

Plasmid

#67846

Purpose

mammalian expression of human V1a receptor with GFP tag

Depositor

Christian Gruber

Article

Koehbach et al Proc Natl Acad Sci U S A. 2013 Dec 24;11

Insert

V1a receptor (AVPR1A Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2015

Availability

Academic Institutions and Nonprofits only
hV1b-GFP-1

Plasmid

#67847

Purpose

mammalian expression of human V1b receptor with GFP tag

Depositor

Christian Gruber

Article

Koehbach et al Proc Natl Acad Sci U S A. 2013 Dec 24;11

Insert

V1b receptor (AVPR1B Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2015

Availability

Academic Institutions and Nonprofits only
pBactin-PACT-mOrange2-hCM1

Plasmid

#196868

Purpose

Expression of Pericentrin-AKAP450 centrosomal targeting (PACT) domain fused to mOrange2 and human (h) Centrosomin motif 1 (CM1). PACT targets CM1 to the centrosome

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

PACT-mOrange2-hCM1 (Akap9 Rat)

Expression

Mammalian

Promoter

Chicken bactin (plus Chicken bactin intron)

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1a-extGluc-mKate-NGFR

Plasmid

#104831

Purpose

extGluc luciferase for invivo Imaging, mKate and NGFR for flourescent detection

Depositor

Irmela Jeremias

Article

Ebinger et al Cancer Cell. 2016 Dec 12;30(6):849-862.

Inserts

mKate
NGFR

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pBactin-AcGFP-128-425-Akap9

Plasmid

#196871

Purpose

Expression of fragment 128-425 from A-Kinase Anchoring Protein 9 (Akap9) fused to AcGFP. Used to displace endogenous Akap9 from Golgi

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

AcGFP-Akap9 (128-425) (Akap9 Rat)

Expression

Mammalian

Promoter

Chicken bactin (plus Chicken bactin intron)

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti TMEM30A

Plasmid

#171789

Purpose

transfer plasmid for lentiviral vector production expressing TMEM30A

Depositor

Veerle Baekelandt

Article

Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

Insert

TMEM30A (TMEM30A Human)

Use

Lentiviral

Tags

6X His + Flag

Promoter

CMV

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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