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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 21,829 results for: SPR

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Showing: 3741 - 3760 of 21829 results

  1. pZ8-P_dCas9

    Plasmid
    #74063
    Purpose
    pZ8-1 plasmid carrying dcas9 driven by the propionate-inducible prpD2 promoter (PprpD2), KanR
    Depositor
    Timothy Lu
    Article
    Cleto et al ACS Synth Biol. 2016 Feb 16.
    Insert
    dcas9
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    Propionate inducible promoter (prp)
    Available Since
    April 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    74063-plasmid-map-sequence-id-141970

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  2. CRISPRi GuEST-List v1

    Pooled Library
    #248188
    Purpose
    The CRISPRi Guest for Enrichment-based Screening of Targets Library (CRISPRi GuEST-List v1) enables genome-wide, single-cell pooled screens conducted with the Flex workflow from 10x Genomics.
    Depositor
    Rahul Satija
    Article
    Bradu et al bioRxiv 2026.02.12.705613
    Expression
    Mammalian
    Species
    Homo sapiens
    Use
    CRISPR and Lentiviral
    Available Since
    Feb. 17, 2026
    Availability
    Academic Institutions and Nonprofits only

  3. dCas9-dMSK1

    Plasmid
    #165602
    Purpose
    Expresses Sp dCas9 fused to truncated human MSK1 (42-802)
    Depositor
    Isaac Hilton
    Article
    Li et al Nat Commun. 2021 Feb 9;12(1):896. doi: 1
    Insert
    S. Pyogenes dCas9 with c-terminal truncated human Mitogen- and stress-activated protein kinase-1 (42-802) (RPS6KA5 Synthetic, Human, S. Pyogenes)
    Use
    CRISPR and Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    165602-plasmid-map-sequence-id-327024

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  4. pAAV-hSyn1-eYFP (AAV2)

    Viral Prep
    #117382-AAV2
    Purpose
    Ready-to-use AAV2 particles produced from pAAV-hSyn1-eYFP (#117382). In addition to the viral particles, you will also receive purified pAAV-hSyn1-eYFP plasmid DNA. hSyn-driven eYFP expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Viviana Gradinaru
    Article
    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:
    Promoter
    Syn
    Tags
    eYFP
    Available Since
    Feb. 14, 2022
    Availability
    Academic Institutions and Nonprofits only

  5. pAAV-hSyn1-eYFP (AAV8)

    Viral Prep
    #117382-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-hSyn1-eYFP (#117382). In addition to the viral particles, you will also receive purified pAAV-hSyn1-eYFP plasmid DNA. hSyn-driven eYFP expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Viviana Gradinaru
    Article
    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:
    Promoter
    Syn
    Tags
    eYFP
    Available Since
    Jan. 6, 2022
    Availability
    Academic Institutions and Nonprofits only

  6. dSpCas9-VPR

    Plasmid
    #205247
    Purpose
    In vitro transcription plasmid for production of dSpCas9-VPR mRNA for transcriptional activation (VPR = tripartite transactivator VP64, p65, and Rta). Insert is derived from Addgene plasmid #63798.
    Depositor
    Rasmus Bak
    Article
    Jensen et al Genome Res. 2021 Nov;31(11):2120-2130. d
    Insert
    dSpCas9-VPR
    Use
    CRISPR
    Available Since
    Sept. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    205247-plasmid-map-sequence-id-408440

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  11. pDG330

    Plasmid
    #100898
    Purpose
    SpCas9 with a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG
    Expression
    Mammalian
    Promoter
    CBh and U6
    Available Since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100898-plasmid-map-sequence-id-195326

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  13. pCT

    Plasmid
    #60620
    Purpose
    Plasmid encoding iCas9 and tracrRNA on pRS415 backbone
    Depositor
    Huimin Zhao
    Article
    Bao et al ACS Synth Biol. 2014 Sep 19.
    Inserts
    iCas9
    tracrRNA
    Use
    CRISPR
    Tags
    FLAG and SV40 NLS
    Expression
    Yeast
    Mutation
    changed Aspartate 147 to Tyrosine, Proline 411 to…
    Promoter
    RPR1p and TEF1p
    Available Since
    Nov. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    60620-plasmid-map-sequence-id-99574

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  18. pMB67

    Plasmid
    #47947
    Purpose
    Expresses C. elegans optimized Cas9 from the hsp-16.48 promoter
    Depositor
    Mike Boxem
    Article
    Waaijers et al Genetics. 2013 Aug 26.
    Insert
    Cas9
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and egl-13 NLS
    Expression
    Worm
    Promoter
    hsp-16.48
    Available Since
    March 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    47947-plasmid-map-sequence-id-68808

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Showing: 3741 - 3760 of 21829 results