We narrowed to 6,744 results for: WUS;

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pBIG2abc_nsp12-His6-3xFlag/nsp7/nsp8 (SARS-CoV-2)

Plasmid

#169185

Purpose

Baculoviral transfer vector to co-express nsp12-His6-3xFlag, nsp7 and nsp8 (SARS-CoV-2) in insect cells

Depositor

John Diffley

Article

Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

Insert

nsp12-His6-3xFlag/nsp7/nsp8 (ORF1ab Synthetic, SARS-CoV-2)

Tags

His6-3xFlag (nsp12 C-terminus)

Expression

Insect

Mutation

Codon optimised for insect cells (Spodoptera frug…

Promoter

Polyhedrin

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pLIB_3xFlag-His6-nsp13 (SARS-CoV-2)

Plasmid

#169189

Purpose

Baculoviral transfer vector to express 3xFlag-His6-nsp13 (SARS-CoV-2) in insect cells

Depositor

John Diffley

Article

Zeng et al Biochem J. 2021 Jul 16;478(13):2405-2423

Insert

3xFlag-His6-nsp13 (ORF1ab Synthetic, SARS-CoV-2)

Tags

3xFlag-His6

Expression

Insect

Mutation

Codon optimised for insect cells (Spodoptera frug…

Promoter

Polyhedrin

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pXZX351

Plasmid

#169454

Purpose

pRS426-SNR52p-gRNA.MATα_option1-SUP4t; 20-bp gRNA sequence targeting MATα cassette

Depositor

Jef Boeke

Article

Xie et al G3 (Bethesda). 2018 Jan 4;8(1):173-183.

Insert

gRNA for MATα

Expression

Yeast

Promoter

SNR52

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ266

Plasmid

#164713

Purpose

Gateway entry clone (attL1 & attR5) PmCDA1-CBE_V01 for C-T base editing

Depositor

Yiping Qi

Article

Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

Insert

zCas9(D10A)-PmCDA1-UGI

Use

CRISPR; Gateway compatible pmcda1-cbe_v01 entry c…

Expression

Plant

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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Th-iCre

Plasmid

#159459

Purpose

Targeting vector to TH gene locus for induction of P2A-fused iCre, with PGK-puroR drug resistant cassette flanked by FRT sequence.

Depositor

Yuejun Chen

Article

Xiong et al Cell Stem Cell. 2020 Sep 16. pii: S1934-

Insert

P2A-iCre

Use

Cre/Lox

Tags

P2A

Expression

Mammalian

Promoter

Endogenous TH

Available Since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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T7pr-His6-MBP-TEV-dCas9-BirA*

Plasmid

#159994

Purpose

Bacterial expression of dCas9-BirA*

Depositor

Jacob Corn

Article

Wang et al Mol Cell. 2020 Jul 16;79(2):221-233.e5.

Insert

dCas9-BirA*

Tags

6X-His, MBP, 3X-FLAG, NLS

Expression

Bacterial

Mutation

D10A, H840A

Promoter

T7

Available Since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV-2 ORF10wu_nostop

Plasmid

#152986

Purpose

Gateway-compatible Entry vector, with insert of ORF10wu gene's CDS from SARS-CoV-2 genome definition in Wu et al, Nature 2020

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

ORF10wu

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available Since

May 27, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223 SARS-CoV-2 ORF9Bwu_nostop

Plasmid

#149328

Purpose

Gateway-compatible Entry vector, with insert of ORF9Bwu gene's CDS from SARS-CoV-2 genome definition in Wu et al, Nature 2020. Does not contain stop codon.

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

ORF9Bwu

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available Since

May 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223_RBM45_WT

Plasmid

#82892

Purpose

Gateway Donor vector containing RBM45, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

RBM45 (RBM45 Human)

Use

Gateway Cloning

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_HAVCR2_WT

Plasmid

#82893

Purpose

Gateway Donor vector containing HAVCR2, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

HAVCR2 (HAVCR2 Human)

Use

Gateway Cloning

Mutation

R140L

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_KEAP1_p.G417R

Plasmid

#82836

Purpose

Gateway Donor vector containing KEAP1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

KEAP1 (KEAP1 Human)

Use

Gateway Cloning

Mutation

G417R; C624S

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PIK3CA_p.E453Q

Plasmid

#82844

Purpose

Gateway Donor vector containing PIK3CA, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

NM_006218 (PIK3CA Human)

Use

Gateway Cloning

Mutation

E453Q

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_KRAS_p.G13V

Plasmid

#82800

Purpose

Gateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

KRAS (KRAS Human)

Use

Gateway Cloning

Mutation

G13V

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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dPspCas13b-mutFTO

Plasmid

#223697

Purpose

Expressing fusion protein of dPspCas13b and inactive mutant of human FTO

Depositor

Chuan He

Article

Wei et al Science. 2022 May 27;376(6596):968-973.

Insert

dPspCas13b-GGS-mutFTO (FTO Human)

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pPHAGE_DLD-H450A-C-TAP

Plasmid

#83478

Purpose

Mammalian expression of the dehydrogenase-deficient mutant DLD-H450A.

Depositor

Mohan Babu

Article

Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

Insert

DLD (DLD Human)

Use

Lentiviral

Tags

TAP

Expression

Mammalian

Mutation

H450A

Promoter

CMV

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pAi14-GFPNLS-MC

Plasmid

#87114

Purpose

minicircle parental backbone plasmid including GFPNLS-pA knock-in donor and one cutting site for HITI

Depositor

Juan Belmonte

Article

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Insert

GFPNLSPpA

Use

CRISPR

Promoter

nEF

Available Since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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AID-BE4max-P2A-GFP

Plasmid

#157948

Purpose

Mammalian expression of AID-BE4max-P2A-GFP

Depositor

Dali Li

Article

Zhang et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

AID-BE4max

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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RKS-Fzd8 subtype NGS Wnt

Plasmid

#159627

Purpose

Express Fzd8 subtype NGS Wnt in mammalian cells

Depositor

Chris Garcia

Article

Miao et al Cell Stem Cell. 2020 Aug 14. pii: S1934-

Insert

Fzd8 subtype NGS Wnt

Use

Lentiviral

Tags

His tag

Expression

Mammalian

Available Since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pGL3-basic-FTO promoter_WT

Plasmid

#108922

Purpose

The core promoter region of FTO was inserted into pGL3 basic vector

Depositor

Jianjun Chen

Article

Su et al Cell. 2018 Jan 11;172(1-2):90-105.e23. d

Insert

FTO (FTO Human)

Tags

NA

Expression

Mammalian

Mutation

NA

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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TK4_Zim3-dCas9-2xMyc-NLS-T2A-mApple_EF1a-Hygro

Plasmid

#254456

Purpose

TK4 PiggyBac plasmid for stable expression of Zim3-dCas9 during human pluripotent stem cell differentiation

Depositor

Marius Wernig

Article

Uenaka et al Cell Stem Cell. 2026 Feb 13:S1934-5909(2

Inserts

Zim3-dCas9-2xMyc-NLS-T2A-mApple
hygroR

Use

Piggybac

Tags

T2A-mApple

Expression

Mammalian

Promoter

CAG and EF1a

Available Since

April 24, 2026

Availability

Academic Institutions and Nonprofits only

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