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pJK_proK14_amiE

Plasmid

#59839

Purpose

Constitutively expresses AmiE with proK14 promoter

Depositor

Timothy Whitehead

Article

Bienick et al PLoS One. 2014 Oct 6;9(10):e109105. doi:

Insert

ALIPHATIC AMIDASE (amiE )

Tags

6x-His

Expression

Bacterial

Promoter

proK14

Available Since

Oct. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pJK_proK9_amiE

Plasmid

#59840

Purpose

Constitutively expresses AmiE with proK9 promoter

Depositor

Timothy Whitehead

Article

Bienick et al PLoS One. 2014 Oct 6;9(10):e109105. doi:

Insert

ALIPHATIC AMIDASE (amiE )

Tags

6x-His

Expression

Bacterial

Promoter

proK9

Available Since

Oct. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pJK_proK6_amiE

Plasmid

#59841

Purpose

Constitutively expresses AmiE with proK6 promoter

Depositor

Timothy Whitehead

Article

Bienick et al PLoS One. 2014 Oct 6;9(10):e109105. doi:

Insert

ALIPHATIC AMIDASE (amiE )

Tags

6x-His

Expression

Bacterial

Promoter

proK6

Available Since

Oct. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pJK_proK3_amiE

Plasmid

#59842

Purpose

Constitutively expresses AmiE with proK3 promoter

Depositor

Timothy Whitehead

Article

Bienick et al PLoS One. 2014 Oct 6;9(10):e109105. doi:

Insert

ALIPHATIC AMIDASE (amiE )

Tags

6x-His

Expression

Bacterial

Promoter

proK3

Available Since

Oct. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pJK_pro6_amiE

Plasmid

#59844

Purpose

Constitutively expresses AmiE with pro6 promoter

Depositor

Timothy Whitehead

Article

Bienick et al PLoS One. 2014 Oct 6;9(10):e109105. doi:

Insert

ALIPHATIC AMIDASE (amiE )

Tags

6x-His

Expression

Bacterial

Promoter

pro6

Available Since

Oct. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-R-Creon shRNA[Control]

Plasmid

#180775

Purpose

This vector simultaneously expresses mCherry protein and shRNA in a Cre-dependent manner.

Depositor

Eun Mi Hwang

Article

Kim et al J Neurosci Methods. 2021 Dec 21:109452.

Type

Empty backbone

Use

AAV, Cre/Lox, and RNAi

Expression

Mammalian

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pLJC5-3XHA-EGFP-PEX26

Plasmid

#139054

Purpose

HA-PEROXO Lentiviral Construct. Expresses a Peroxisomal Tag.

Depositor

David Sabatini

Article

Ray et al iScience. 2020 May 22;23(5):101109. doi:

Insert

3XHA-EGFP-PEX26

Use

Lentiviral

Tags

HA

Promoter

UBC

Available Since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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Fuw PBRM1 mBD5

Plasmid

#121109

Purpose

conditional expression

Depositor

Emily Dykhuizen

Article

Porter et al J Biol Chem. 2017 Jan 4. pii: jbc.M116.7

Insert

PBRM1 (N739A) (PBRM1 Human)

Use

Lentiviral

Mutation

PBRM1 (N739A)

Available Since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pMA4008

Plasmid

#70109

Purpose

mtDNA destruction/production of rho-0 cells

Depositor

Mikhail Alexeyev

Article

Spadafora et al PLoS One. 2016 May 2;11(5):e0154684. doi

Inserts

EGFP
Herpes simplex virus UL12.5M185

Tags

none

Expression

Mammalian

Mutation

N-terminal deletion of UL12.5

Promoter

CMV and RSV

Available Since

May 3, 2016

Availability

Academic Institutions and Nonprofits only

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pBLind

Plasmid

#113109

Purpose

Vector encoding the RFP gene under blue light-inducible promoter BindF1.

Depositor

Chueh Loo Poh

Article

Jayaraman et al ACS Synth Biol. 2018 Apr 20;7(4):986-994

Insert

RFP

Use

Cell-free

Expression

Bacterial

Promoter

BindF1

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-G-Creon shRNA[Control]

Plasmid

#181824

Purpose

This vector simultaneously expresses GFP protein and shRNA in a Cre-dependent manner.

Depositor

Eun Mi Hwang

Article

Kim et al J Neurosci Methods. 2021 Dec 21:109452.

Type

Empty backbone

Use

AAV and RNAi

Expression

Mammalian

Promoter

EF1a/U6

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pLJC5-3XMyc-EGFP-PEX26

Plasmid

#139059

Purpose

Myc-PEROXO Lentiviral Construct. Expresses a Peroxisomal Tag.

Depositor

David Sabatini

Article

Ray et al iScience. 2020 May 22;23(5):101109. doi:

Insert

3XMyc-EGFP-PEX26

Use

Lentiviral

Tags

Myc

Promoter

UBC

Available Since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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pHSV-TK

Plasmid

#165801

Purpose

Minimal viral terminator derived from Herpes Simplex 1 thymidine kinase gene

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

HSV-TK

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mNG-IRES-mScarlet-dPCP

Plasmid

#231090

Purpose

Bicistronic construct encoding mNG in combination with mScarlet-dPCP via an EMCV-IRES sequence.

Depositor

John Ngo

Article

Kuffner et al Nat Methods. 2025 Sep 22. doi: 10.1038/s

Insert

mNG-IRES-mScarlet-dPCP

Expression

Mammalian

Available Since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pRDA_867

Plasmid

#231109

Purpose

A>G base editor

Depositor

John Doench, David Root

Article

Kaplan et al Nat Genet. 2025 Nov;57(11):2920-2929. do

Insert

ABE8e-nCas9-SpG-HA

Use

CRISPR and Lentiviral

Mutation

D10A

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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VPS13D^EGFP

Plasmid

#174109

Purpose

The insert is a codon optimized DNA sequence encoding VPS13D with an internal EGFP.

Depositor

Pietro De Camilli

Article

Guillen-Samander et al J Cell Biol. 2021 May 3;220(5). pii: 212

Insert

Codon optimized VPS13D

Tags

EGFP

Expression

Mammalian

Mutation

With an internal EGFP tag in aminoacid position 1…

Promoter

CMV

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pET21a-GluRS-His

Plasmid

#124109

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Glutamyl-tRNA ligase (gltX Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pmTurquoise2-Drp1

Plasmid

#110946

Purpose

Expresses dynamin-like protein 1 fused to mTurquoise2

Depositor

Pablo Rivera-Fuentes

Insert

Dynamin-1-like protein (DNM1L Human)

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5_FRT_mCH

Plasmid

#127109

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

mCherry

Expression

Mammalian

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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FR_HNF6

Plasmid

#31099

Depositor

Gerhart Ryffel

Article

Thomas et al Nucleic Acids Res. 2004 . 32(19):e150.

Insert

HNF6 (ONECUT1 Human)

Tags

His

Expression

Mammalian

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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