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pAT847

Plasmid

#68935

Purpose

Memory arrary integration vector for insertion between BT2113 and BT2114 in the B. thetaiotaomicron chromosome, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Insert

Integrase Memory Array

Use

Integration vector

Available Since

Oct. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pBBRBB-Ppuf843-1200-PR

Plasmid

#50963

Purpose

Expresses proteorhodopsin using the Ppuf843-1200 promoter segment, for use in R. sphaeroides

Depositor

Claudia Schmidt-dannert

Article

Tikh et al Appl Microbiol Biotechnol. 2014 Feb 9.

Insert

Proteorhodopsin

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Promoter

Ppuf843-1200

Available Since

Feb. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pBluescript PMS1

Plasmid

#16457

Depositor

Bert Vogelstein

Article

Nicolaides et al Nature. 1994 Sep 1. 371(6492):75-80.

Insert

PMS1 (PMS1 Human)

Expression

Bacterial

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pMM705

Plasmid

#68892

Purpose

IPTG-inducible CRISPRi vector targeting BT1754, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Inserts

dCas9
LacI
sgRNA

Available Since

Oct. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-miR29b(m4)

Plasmid

#21122

Depositor

Joshua Mendell

Article

Hwang et al Science. 2007 Jan 5. 315(5808):97-100.

Insert

miR-29b-1 (MIR29B1 Human)

Expression

Mammalian

Mutation

G to C change at nucleotide 21 of the mature miR-…

Available Since

May 15, 2009

Availability

Academic Institutions and Nonprofits only
pCZGY2162

Plasmid

#110136

Purpose

Epidermal Mitochondrial-tagged Florescent Calcium Sensor

Depositor

Andrew Chisholm

Article

Xu et al Dev Cell. 2014 Oct 13;31(1):48-60. doi:

Insert

mito::GCaMP3

Tags

mito::GCaMP3

Expression

Worm

Promoter

Pcol-19

Available Since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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pAJ08

Plasmid

#14912

Depositor

James Lok

Article

Junio et al Exp Parasitol. 2008 Feb . 118(2):253-65.

Insert

strongyloides stercoralis actin promoter

Tags

GFP

Expression

Worm

Available Since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

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pBBRBB-Ppuf1-1200-DsRed

Plasmid

#50960

Purpose

Expressed DsRed using the Ppuf1-1200 promoter segment, for use in R. sphaeroides

Depositor

Claudia Schmidt-dannert

Article

Tikh et al Appl Microbiol Biotechnol. 2014 Feb 9.

Insert

DsRed

Use

Synthetic Biology

Promoter

Ppuf1-1200

Available Since

March 5, 2014

Availability

Academic Institutions and Nonprofits only

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pET26-CNTnw

Plasmid

#100163

Purpose

recombinant expression of target protein as an MBP-His tag fusion at the N-terminus of target protein

Depositor

Seok-Yong Lee

Article

Hirschi et al Nature. 2017 May 4;545(7652):66-70. doi:

Insert

Concentrative nucleoside transporter

Tags

MBP-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pVY-3 sfGFP-tag3

Plasmid

#194618

Purpose

fluorescent proteins with cationic peptide tags to promote biomolecular condensate formation in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al Nat Commun. 2022 Dec 22;13(1):7882. doi:

Insert

sfGFP-K(GGSKKRKKR)3

Tags

KGGSKKRKKRGGSKKRKKRGGSKKRKKR and MGHHHHHGGA

Expression

Bacterial

Promoter

T7

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pET26-CNTN149L

Plasmid

#100164

Purpose

recombinant expression of target protein as an MBP-His tag fusion at the N-terminus of target protein

Depositor

Seok-Yong Lee

Article

Hirschi et al Nature. 2017 May 4;545(7652):66-70. doi:

Insert

Concentrative nucleoside transporter

Tags

MBP-His

Expression

Bacterial

Mutation

N149L

Promoter

T7

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pAc5.1-PH-mEGFP (FspAI) (JM50)

Plasmid

#206450

Purpose

mEGFP expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

mEGFP

Expression

Insect

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAN012

Plasmid

#220051

Purpose

Reporter plasmid that expresses GFP and firefly luciferase linked with a viral 2A peptide (positive control).

Depositor

Blake Wiedenheft

Article

Nemudraia et al Science. 2024 Apr 25:eadk5518. doi: 10.1

Insert

GFP-fLuc

Expression

Mammalian

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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pVY-17 TC-TEM1-tag3

Plasmid

#194631

Purpose

antibiotic resistance enzyme with cationic peptide tag and tetracysteine tag

Depositor

Allie Obermeyer

Article

Yeong et al Nat Commun. 2022 Dec 22;13(1):7882. doi:

Insert

tetracysteine-TEM1-GGSKKRKKRGGSKKRKKRGGSKKRKKR

Tags

GGSKKRKKRGGSKKRKKRGGSKKRKKR and MGCCPGCCGGA

Expression

Bacterial

Promoter

pBAD

Available Since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pVY-19 TC-sfGFP-tag3

Plasmid

#194633

Purpose

fluorescent protein with cationic peptide tag and tetracysteine tag

Depositor

Allie Obermeyer

Article

Yeong et al Nat Commun. 2022 Dec 22;13(1):7882. doi:

Insert

tetracysteine-sfGFP-K(GGSKKRKKR)3

Tags

KGGSKKRKKRGGSKKRKKRGGSKKRKKR and MGCCPGCCGGA

Expression

Bacterial

Promoter

T7

Available Since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pVY-15 TC-CAT-tag3

Plasmid

#194629

Purpose

antibiotic resistance enzyme with cationic peptide tag and tetracysteine tag

Depositor

Allie Obermeyer

Article

Yeong et al Nat Commun. 2022 Dec 22;13(1):7882. doi:

Insert

tetracysteine-CAT-(GGSKKRKKR)3

Tags

GGSKKRKKRGGSKKRKKRGGSKKRKKR and MGCCPGCCGGA

Expression

Bacterial

Promoter

T7

Available Since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pVY-11 mScarletI-tag3

Plasmid

#194626

Purpose

fluorescent proteins with cationic peptide tags to promote biomolecular condensate formation in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al Nat Commun. 2022 Dec 22;13(1):7882. doi:

Insert

mScarlet-I-(GGSKKRKKR)3

Tags

GGSKKRKKRGGSKKRKKRGGSKKRKKR and MGHHHHHGGA

Expression

Bacterial

Promoter

T7

Available Since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pVY-10 mScarletI-tag2

Plasmid

#194625

Purpose

fluorescent proteins with cationic peptide tags to promote biomolecular condensate formation in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al Nat Commun. 2022 Dec 22;13(1):7882. doi:

Insert

mScarlet-I-(GGSKKRKKR)2

Tags

GGSKKRKKRGGSKKRKKR and MGHHHHHGGA

Expression

Bacterial

Promoter

T7

Available Since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pVY-9 GFP(+6)-tag3

Plasmid

#194624

Purpose

fluorescent proteins with cationic peptide tags to promote biomolecular condensate formation in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al Nat Commun. 2022 Dec 22;13(1):7882. doi:

Insert

GFP(+6)-(GGSKKRKKR)3

Tags

GGSKKRKKRGGSKKRKKRGGSKKRKKR and MGHHHHHGGA

Expression

Bacterial

Promoter

T7

Available Since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pVY-8 GFP(+6)-tag2

Plasmid

#194623

Purpose

fluorescent proteins with cationic peptide tags to promote biomolecular condensate formation in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al Nat Commun. 2022 Dec 22;13(1):7882. doi:

Insert

GFP(+6)-(GGSKKRKKR)2

Tags

GGSKKRKKRGGSKKRKKR and MGHHHHHGGA

Expression

Bacterial

Promoter

T7

Available Since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

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