Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

We narrowed to 1,257 results for: LIPI

Showing: 901 - 950 of 1257 results

  1. Cav3P104L-YFP

    Plasmid
    #68404
    Purpose
    Expression of fluorescently tagged Caveolin3 point mutation in mammalian cells
    Depositor
    Rob Parton
    Article
    Pol et al Mol Biol Cell. 2005 Apr;16(4):2091-105.
    Insert
    Cav3 (Cav3 Mouse)
    Use
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    Proline at position 104 changed to a Leucine
    Promoter
    CMV
    Available since
    Sept. 21, 2015
    Availability
    Academic Institutions and Nonprofits only

  6. pcDNA3-Lyso-InPGrp

    Plasmid
    #236002
    Purpose
    Genetically encoded FRET-based phosphoinositide sensor for monitoring PI(3,4,5)P3. Targeted to lysosome surface.
    Depositor
    Jin Zhang
    Article
    Sahan et al Proc Natl Acad Sci U S A. 2025 Apr;122(1
    Insert
    Lyso-InPGrp (Cyth3 Synthetic, Rat)
    Use
    Tags
    Cerulean, Full-length LAMP1, and cpVenus[E172]
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    236002-plasmid-map-sequence-id-473795

    Enlarge

  7. pcDNA3-Endo-INPP5E

    Plasmid
    #236005
    Purpose
    Endosome-targeted inositol polyphosphate 5 phosphatase (INPP5E); hydrolyzes 5-phosphate in PI(3,4,5)P3 and PI(4,5)P2.
    Depositor
    Jin Zhang
    Article
    Sahan et al Proc Natl Acad Sci U S A. 2025 Apr;122(1
    Insert
    Endo-INPP5E (INPP5E Synthetic, Human)
    Use
    Tags
    2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and mChe…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    236005-plasmid-map-sequence-id-473813

    Enlarge

  11. pHIE323-LPIV Puro-P2A-miRFP720 ZF6x12-C-DsRed-Express2(x6) phEF1α-BlastR

    Plasmid
    #201539
    Purpose
    Landing pad integration vector used to generate ZF6 synTF reporter line (HIE156), assembled with pPD1178 (destination vector), pHIE324-329 (TUPV1-6), pHIE280 (TUPV7), pPD1157 (linker)
    Depositor
    Joshua Leonard
    Article
    Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:
    Inserts
    PuromycinR-P2A-miRFP720
    ZF6x12-C-DsRed-Express2(x6)
    BlasticidinR
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    None, ZF6x12(C)_YB_TATA Minimal Promoter, and hEF…
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    201539-plasmid-map-sequence-id-401324

    Enlarge

  13. pcDNA5/FRT/TO GABARAP(G116A)-mTagBFP2-GABARAP(G116A)

    Plasmid
    #212108
    Purpose
    Expression of lipidation deficient GABARAP split tandem protein with mTagBFP2
    Depositor
    Dieter Willbold
    Article
    Üffing et al Autophagy Reports, 3(1). doi: 10.1080/27
    Insert
    GABARAP(G116A)-mTagBFP2-GABARAP(G116A) (GABARAP Entacmaea quadricolor, Human)
    Use
    Tags
    mTagBFP2
    Expression
    Mammalian
    Mutation
    G116A (lipidation deficiency) of both GABARAPs
    Promoter
    CMV
    Available since
    May 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    212108-plasmid-map-sequence-id-420140

    Enlarge

  19. p3E-DrCavin1a

    Plasmid
    #194291
    Purpose
    Multisite gateway entry clone for expression of codon optimised zebrafish cavin1a with fusion tag at the N-terminus. Parton lab clone KXM
    Depositor
    Rob Parton
    Article
    Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.
    Insert
    cavin1a (cavin1a Zebrafish)
    Use
    Multisite gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    194291-plasmid-map-sequence-id-380041

    Enlarge

  22. pCu-His6-Atg13[571–700] K683E, H685E, K686E-Ss(424)

    Plasmid
    #166855
    Purpose
    Expresses 6xHis tagged ATG13 [571–700] fragment mutated in K683E, H685E, K686E with 14 scramble amino acid sequence DIKLIDTVDLESCN under a Cu promoter in yeast
    Depositor
    Daniel Klionsky
    Article
    Gatica et al Autophagy. 2020 Jun;16(6):1007-1020. doi
    Insert
    Atg13[571–700] (ATG13 Budding Yeast)
    Use
    Tags
    6xHis
    Expression
    Yeast
    Mutation
    K683E, H685E, K686E
    Promoter
    pCu
    Available since
    April 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    166855-plasmid-map-sequence-id-329426

    Enlarge

  23. pCu-His6-Atg13[571–700] F641E, I645E-Ss(424)

    Plasmid
    #166854
    Purpose
    Expresses 6xHis tagged ATG13 [571–700] fragment mutated in F641E, I645E with 14 scramble amino acid sequence DIKLIDTVDLESCN under a Cu promoter in yeast
    Depositor
    Daniel Klionsky
    Article
    Gatica et al Autophagy. 2020 Jun;16(6):1007-1020. doi
    Insert
    Atg13[571–700] (ATG13 Budding Yeast)
    Use
    Tags
    6xHis
    Expression
    Yeast
    Mutation
    F641E, I645E
    Promoter
    pCu
    Available since
    April 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    166854-plasmid-map-sequence-id-329424

    Enlarge

  24. pCu-His6-Atg13[571–700] F641G, I645G-Ss(424)

    Plasmid
    #166853
    Purpose
    Expresses 6xHis tagged ATG13 [571–700] fragment mutated in F641G, I645G with 14 scramble amino acid sequence DIKLIDTVDLESCN under a Cu promoter in yeast
    Depositor
    Daniel Klionsky
    Article
    Gatica et al Autophagy. 2020 Jun;16(6):1007-1020. doi
    Insert
    Atg13[571–700] (ATG13 Budding Yeast)
    Use
    Tags
    6xHis
    Expression
    Yeast
    Mutation
    F641G, I645G
    Promoter
    pCu
    Available since
    April 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    166853-plasmid-map-sequence-id-329422

    Enlarge

  25. pPICZ-His6-K2P4.1B-mCherry-StreptagII

    Plasmid
    #158744
    Purpose
    Expresses K2P4.1 isoform B with TEV protease cleavable N- and C-terminal affinity tags.
    Depositor
    Arthur Laganowsky
    Article
    Schrecke et al Nat Chem Biol. 2021 Jan;17(1):89-95. doi
    Insert
    K2P4.1-B (KCNK4 Human)
    Use
    Tags
    His6 and mCherry with Streptag II
    Expression
    Yeast
    Mutation
    Residues 1-290 and N-linked glycosylation sites r…
    Promoter
    AOX1
    Available since
    Sept. 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    158744-plasmid-map-sequence-id-309639

    Enlarge

  34. LCV2_EGFP

    Plasmid
    #155098
    Purpose
    Lentiviral backbone for cloning and expression of U6 driven gRNAs with Esp3I/BsmBI cloning sites, puromycin selection and EGFP expression
    Depositor
    Jason Moffat
    Article
    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    155098-plasmid-map-sequence-id-307983

    Enlarge

  36. LCV2_mCherry

    Plasmid
    #155096
    Purpose
    Lentiviral backbone for cloning and expression of U6 driven gRNAs with Esp3I/BsmBI cloning sites, puromycin selection and mCherry expression
    Depositor
    Jason Moffat
    Article
    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    155096-plasmid-map-sequence-id-307998

    Enlarge

  37. LCV2_mClover3

    Plasmid
    #155095
    Purpose
    Lentiviral backbone for cloning and expression of U6 driven gRNAs with Esp3I/BsmBI cloning sites, puromycin selection and mClover3 expression
    Depositor
    Jason Moffat
    Article
    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    155095-plasmid-map-sequence-id-308012

    Enlarge

  41. pCAG-FLAG-puro-PTPIP51(1-150_175-470)

    Plasmid
    #170537
    Purpose
    Expresses PTPIP51 FFAT-like motif (151-175) deletion mutant (1-150_176-470) in human HeLa cells after transfection
    Depositor
    Byung Il Lee
    Article
    Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:
    Insert
    Protein tyrosine phosphatase interacting protein 51 (RMDN3 Human)
    Use
    Tags
    FLAG tag
    Expression
    Mammalian
    Mutation
    deleted amino acids 151-175
    Promoter
    CAG promoter
    Available since
    Aug. 4, 2021
    Availability
    Academic Institutions and Nonprofits only

  44. Flag_HsIRE1a_deltaP29_D408_pBabePuro

    Plasmid
    #58422
    Purpose
    retroviral pBabe puro vector encoding N-terminally FLAG tagged mutant human IRE1a deleted from amino acids P28 to D408
    Depositor
    David Ron
    Article
    Volmer et al Proc Natl Acad Sci U S A. 2013 Mar 19;11
    Insert
    IRE1a (ERN1 Human)
    Use
    Retroviral
    Tags
    FLAG and preprotrypsin signal sequence
    Expression
    Mammalian
    Mutation
    Deleted amino acids P29 to D408
    Promoter
    Available since
    Sept. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    58422-plasmid-map-sequence-id-90253

    Enlarge

  50. LCV2_BFP2

    Plasmid
    #155097
    Purpose
    Lentiviral backbone for cloning and expression of U6 driven gRNAs with Esp3I/BsmBI cloning sites, puromycin selection and BFP2 expression
    Depositor
    Jason Moffat
    Article
    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    155097-plasmid-map-sequence-id-308002

    Enlarge

Showing: 901 - 950 of 1257 results