164,890 results
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Plasmid#168817PurposeGateway-compatible Entry vectorDepositorInsertMERS-CoV-nsp4 (orf1ab )
UseGateway-compatible entry vectorTagsExpressionMutationPromoterAvailable sinceMay 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-eGFP-hTau AP- WPRE
Plasmid#233044PurposeTo Express a GFP human Tau AP fusion protein from a CMV promoterDepositorInserteGFP-hTau AP fusion (MAPT Human, Synthetic)
UseLentiviralTagsEGFPExpressionMutationPromoterAvailable sinceMarch 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEND-321_Cacnes_ori
Plasmid#225603PurposeCutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a high-copy pBR322 E. coli oriDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEND-350_Cacnes_ori
Plasmid#225605PurposeCutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a medium-copy pBR322 E. coli ori (no ROP)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEND-446_Cacnes_ProA
Plasmid#225609PurposeSuicide C. acnes vector to generate ProA (PPA_RS04250) deletion leaving an Ery resistance cassette flanked by BxbI recombination sitesDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEND-224_Cacnes_sfGFP
Plasmid#225611PurposeReplicative vector for green fluorescent reporter protein sfGFP strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+sfGFP.DepositorInsertsfGFP
UseSynthetic BiologyTags6xHisExpressionBacterialMutationCodon optimized for Cutibacterium acnesPromoterAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTlpA_mCherry_Txe/Axe
Plasmid#229147PurposeToxin-Antitoxin (Txe/Axe)DepositorInsertmCherry, Txe/Axe
UseTagsExpressionBacterialMutationPromoterPtlpA (mCherry), Txe/Axe (Native)Available sinceJan. 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-GST-2
Plasmid#228984PurposeFor bacterial expression of anti-GST nanobody GST-2, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.DepositorInsertGST-2
UseTags6xHISExpressionBacterialMutationPromoterT7Available sinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG94-1--LaG94-14
Plasmid#228973PurposeFor bacterial expression of anti-GFP nanobody dimer of LaG94-1 and LaG94-14, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.DepositorInsertLaG94-1--LaG94-14
UseTags6xHISExpressionBacterialMutationPromoterT7Available sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-GST-1
Plasmid#228983PurposeFor bacterial expression of anti-GST nanobody GST-1, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.DepositorInsertGST-1
UseTags6xHISExpressionBacterialMutationPromoterT7Available sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaTdT-1
Plasmid#228974PurposeFor bacterial expression of anti-tdTomato nanobody LaTdT-1, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.DepositorInsertLaTdT-1
UseTags6xHISExpressionBacterialMutationPromoterT7Available sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaRIgG-7
Plasmid#228997PurposeFor bacterial expression of anti-rabbit IgG nanobody LaRIgG-7, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.DepositorInsertLaRIgG-7
UseTags6xHISExpressionBacterialMutationPromoterT7Available sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaRIgG-1
Plasmid#228995PurposeFor bacterial expression of anti-rabbit IgG nanobody LaRIgG-1, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.DepositorInsertLaRIgG-1
UseTags6xHISExpressionBacterialMutationPromoterT7Available sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaMIgG-5
Plasmid#228991PurposeFor bacterial expression of anti-mouse IgG nanobody LaMIgG-5, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.DepositorInsertLaMIgG-5
UseTags6xHISExpressionBacterialMutationPromoterT7Available sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
mCit(P5f-P13f)3-gs
Plasmid#228080PurposeMammalian expression of coiled coil segments (P5f-P13f)x3, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.DepositorInsertmCit, P5f, P13f
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCD-TVMVP
Plasmid#226226PurposeExpress TVMVPDepositorInsertTVMVP (TVMVgp1 Tobacco vein mottling virus)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
PCR4-beta-globin::lacZ-H11
Plasmid#227000PurposeenSERT LacZ reporter vector for site-specific integration into the H11 locus (contains human beta-globin minimal promoter)DepositorTypeEmpty backboneUseCRISPR and Mouse TargetingTagsExpressionMammalianMutationPromoterbeta-globinAvailable sinceNov. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
TagBFP(P6f)3(P14f)3-gs
Plasmid#228081PurposeMammalian expression of coiled coil segments (P6f)x3-(P14f)x3, with short flexible linkers and N-term TagBFP; for phase separation and forming of liquid condensates in mammalian cells.DepositorInsertBFP, P6f, P14f
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
Zed_vector_tol2_-2.1prox1a_1:EGFP_XCA:DsRed2
Plasmid#218207PurposeZebrafish -2.1prox1a_1 (mouse conserved) enhancer reporter in the lymphatic valve from 3 dpf. XCA:DsRed2 as a control in the skeletal and cardiac muscle. Shows the high background typical of the ZED vectorDepositorInsert-2.1prox1a_1
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_hCAR.Variant3
Plasmid#209133PurposeThe ORF of human constitutive androstane receptor (CAR), variant 3 cDNA amplified from the RT human liver RNA was cloned at the NheI/HindIII sites of the pcDNA3.1(+), the mammalian expression vector.DepositorInsertconstitutive androstane receptor (CAR), Variant 3 (NR1I3 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 MERS-CoV NSP6_stop
Plasmid#168633PurposeGateway-compatible Entry vectorDepositorInsertNSP6 (orf1ab )
UseOtherTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223 MERS-CoV NSP3_nostop
Plasmid#168608PurposeGateway-compatible Entry vectorDepositorInsertNSP3 (orf1ab )
UseOtherTagsExpressionMutationPromoterAvailable sinceJan. 20, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
Kv1.2 K+ channel scFv [K14/16]
Plasmid#182064PurposeMammalian Expression of Kv1.2 K+ channel scFv. Derived from hybridoma K14/16.DepositorInsertrecombinant mouse scFv targeting Kv1.2 K+ channel (Homo sapiens) (KCNA2 Mouse)
UseTagsHA, HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIRESN2_GFP_YTA_Champ1_328-354
Plasmid#105639PurposeExpresses fragment (amnio acids 328-354) of human Champ1 protein tagged with GFPDepositorInsertChamp1 (CHAMP1 Human)
UseTagsGFP YTAExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
BASIC_SEVA_5a7_pKD46.10
Plasmid#178937PurposeBASIC DNA assembly vectorDepositorInsertmScarlet counter-selection cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 7, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
CAG-EnRRbpj
Plasmid#160925Purposedominant-negative construct to inhibit Notch signalingDepositorInsertEnRRbpj
UseTagsExpressionMutationfused with an engrailed repressorPromoterAvailable sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Flag-mRIPK1-S321A
Plasmid#115339PurposeMammalian expression of Flag-mRIPK1-S321ADepositorInsertRIPK1 (Ripk1 Mouse)
UseTagsFlagExpressionMammalianMutationS321APromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pet30b-7M SrtA
Plasmid#51141Purposeexpression plasmid for C-terminal his tagged, calcium independent S.aureus SrtA with enhanced catalytic activityDepositorInsertS.aureus SrtA 7M
UseTags6x HisExpressionBacterialMutationP94R, E105K, E108Q, D160N, D165A, K190E, K196TPromoterT7Available sinceFeb. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-Puro-PNPT1 S484A
Plasmid#223316PurposeLentiviral vector expressing human PNPT1 S484A variantDepositorInsertPolyribonucleotide Nucleotidyltransferase 1 (PNPT1 Human)
UseLentiviralTagsExpressionMammalianMutationChanged serine 484 to alaninePromoterAvailable sinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pHR_XPR2_hrGFP
Plasmid#84614PurposeHR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into XPR2 locusDepositorInserthrGFP
UseSynthetic BiologyTagsExpressionYeastMutationPromoterUAS1B8-TEF(136)Available sinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-Neo-PIP-NLS-mVenus
Plasmid#118619PurposeLentiviral expression of the cell cycle marker PIP-NLS-mVenusDepositorInsertPIP-NLS-mVenus
UseLentiviralTagsmVenusExpressionMammalianMutationPromoterhPGKAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDRM166 LKB (Luciferase mKate2 Blast)
Plasmid#183502PurposeExpresses the firefly luciferase gene, the NLS-tagged mKate2 gene, and the blasticidin resistance gene joined by P2A linkers.DepositorInsertsFirefly Luciferase
mKate2
BSD
UseLentiviral and LuciferaseTagsP2A linker and SV40 NLS with GGS linkerExpressionMammalianMutationPromoterMND, MND (off Luc-P2A), and MND (off Luc-P2A-mKat…Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBMN YFP-FKBP(DD)
Plasmid#31766DepositorInsertHA-YFP-FKBP(DD) (FKBP1A Human)
UseRetroviralTagsHA and YFPExpressionMutationE31G, F36V, R71G and K105E on FKBP12PromoterLTRAvailable sinceOct. 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-TagBFP-PuroR
Plasmid#80969PurposeCRISPaint gene taggingDepositorInsertTagBFP
UseGene taggingTagsExpressionMutationPromoternoneAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-mCherry (AAV8)
Viral Prep#114470-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-Ef1a-mCherry (#114470). In addition to the viral particles, you will also receive purified pAAV-Ef1a-mCherry plasmid DNA. EF1a-driven mCherry expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsmCherryAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-DN-hCUL3-FLAG
Plasmid#15820DepositorInsertCullin3 (CUL3 Human)
UseTagsFlagExpressionMammalianMutationDominant negative: N418PromoterAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET Biotin His6 mCherry LIC cloning vector (H6-mCherry)
Plasmid#29722DepositorTypeEmpty backboneUseTags6xHis, AviTag (biotin), TEV cleavage site, and mC…ExpressionBacterialMutationPromoterT7-lacO (lactose/IPTG inducible)Available sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHEN2-SG4 R105A
Plasmid#196072PurposeExpress mutant (R105A) SG4 in E.coliDepositorInsertSG4 R105A
UseTags3XFLAG and 6XHisExpressionBacterialMutationR105A mutation in SG4PromoterT7Available sinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDB179-DuraPETase
Plasmid#218699PurposeEncodes codon optimized DuraPETase enzyme for bacterial expressionDepositorInsertDuraPETase
UseTags10xHisExpressionBacterialMutationPromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-TFEBS211A-MYC (#805)
Plasmid#99957Purposemammalian expressionDepositorInsertTFEB (TFEB Human)
UseTags6xHIs and MycExpressionMammalianMutationS211APromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFC334
Plasmid#87846PurposeAMA1 plasmid with Aspergillus optimized Cas9, argB selection marker and yA specific sgRNA expressed with ribozymesDepositorInsertsCas9
argB
yA specific sgRNA
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationPromoterA. nidulans gpdA promoter with Hammerhead ribozym…Available sinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 ZoAl N-1F
Plasmid#203763PurposeFor expression of N-flag tagged ZoAl R2 protein in mammalian cellsDepositorInsertR2 retroelement protein
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV HA hRB-wt
Plasmid#58905Purposemammalian expression of wild type human RbDepositorInsertRb (RB1 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
spMSP1D1
Plasmid#173482PurposeExpresses spMSP1D1 in Bl21DepositorInsertspMSP1D1
UseTagsHistagExpressionBacterialMutationPromoterAvailable sinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE
Plasmid#202199PurposeExpresses bicistronically soma-targeted ChrimsonR in frame with EGFP and the optimized PdCO under control of human synapsin1 promotor after Cre-dependent recombinationDepositorHas ServiceAAV1 and AAV5InsertstChrimsonR, PdCO
UseAAV and Cre/LoxTagsEGFP and Rho1D4ExpressionMammalianMutationPromoterhSyn1Available sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
GalT-RpHLuorin2
Plasmid#171719PurposeTrans-Golgi expression of ratiometric pHLuorin2DepositorInsertRatiometric pHLuorin2 fused to B4GALT1 (B4GALT1 Human)
UseTagsratiometric pHluorin2ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-6xHis-MBP-TEV site-TadA8.20
Plasmid#194702PurposeExpresses N-terminal MBP fused TadA8.20 in bacteriaDepositorInsertTadA8.20
UseTags6xHis and MBPExpressionBacterialMutationmutations in TadA are described in the paperPromoterT7 promoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRVL-5
Plasmid#104583PurposeContains human IgG1 CH1/CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Full Fc-mediated immune effector functionsDepositorInsertHuman IgG1 CH1/CH2/CH3 (IGHG1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
Human SERCA2b (pcDNA3.1+)
Plasmid#75188PurposeMammalian expression of ATP2A2DepositorInsertATP2A2 (ATP2A2 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only