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291,538 results

Showing: 9501 - 9550 of 291538 results

  4. OmpA3-PNGaseF-TEV-His6

    Plasmid
    #114274
    Purpose
    Amidase removing almost all N-linked oligosaccharides from glycoproteins. This PNGase F construct can have its hexa-his removed.
    Depositor
    Martin Caffrey
    Insert
    Peptide: N-glycosidase F
    Tags
    OmpA3 leader sequence (for periplasmic localisati…
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    114274-plasmid-map-sequence-id-218322

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  5. OmpA3-PNGaseF-TEV-His6

    Plasmid
    #114274
    Purpose
    Amidase removing almost all N-linked oligosaccharides from glycoproteins. This PNGase F construct can have its hexa-his removed.
    Depositor
    Martin Caffrey
    Insert
    Peptide: N-glycosidase F
    Tags
    OmpA3 leader sequence (for periplasmic localisati…
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    114274-plasmid-map-sequence-id-218322

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  12. pTP1174

    Plasmid
    #104162
    Purpose
    Bacterial expression plasmid of anti-mouse kappa chain nanobody TP1170 (1x Cysteine)
    Depositor
    Dirk Görlich
    Publication
    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154
    Insert
    Anti-mouse kappa chain specific nanobody TP1170 (1xCysteine)
    Tags
    14xHistidine tag and NEDD8 from Brachypodium dist…
    Expression
    Bacterial
    Available since
    Dec. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    104162-plasmid-map-sequence-id-200079

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  13. pTP1174

    Plasmid
    #104162
    Purpose
    Bacterial expression plasmid of anti-mouse kappa chain nanobody TP1170 (1x Cysteine)
    Depositor
    Dirk Görlich
    Publication
    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154
    Insert
    Anti-mouse kappa chain specific nanobody TP1170 (1xCysteine)
    Tags
    14xHistidine tag and NEDD8 from Brachypodium dist…
    Expression
    Bacterial
    Available since
    Dec. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    104162-plasmid-map-sequence-id-200079

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  16. p37-2iDMD-LR

    Plasmid
    #88892
    Purpose
    37°C full-length DMD luc-red plasmid. Co-expresses human dystrophin, firefly luciferase and mCherry in mammalian cells. Plasmid carries phiC31 and Bxb1 attB sites and can be grown at 37°C.
    Depositor
    Michele Calos
    Publication
    Farruggio et al Biotechnol J. 2017 Jan 31. doi: 10.1002/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Dystrophin (DMD Human)
    luciferase
    mCherry
    Expression
    Mammalian
    Available since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    88892-plasmid-map-sequence-id-451853

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  17. p37-2iDMD-LR

    Plasmid
    #88892
    Purpose
    37°C full-length DMD luc-red plasmid. Co-expresses human dystrophin, firefly luciferase and mCherry in mammalian cells. Plasmid carries phiC31 and Bxb1 attB sites and can be grown at 37°C.
    Depositor
    Michele Calos
    Publication
    Farruggio et al Biotechnol J. 2017 Jan 31. doi: 10.1002/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Dystrophin (DMD Human)
    luciferase
    mCherry
    Expression
    Mammalian
    Available since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    88892-plasmid-map-sequence-id-451853

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  20. SQT1665

    Plasmid
    #78744
    Purpose
    Human expression plasmid for LbCpf1-NLS-3xHA
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mammalian codon-optimized Lachnospiraceae bacterium ND2006 Cpf1-NLS-3xHA
    Use
    CRISPR
    Tags
    NLS-3xHA
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    June 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    78744-plasmid-map-sequence-id-147455

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  21. SQT1665

    Plasmid
    #78744
    Purpose
    Human expression plasmid for LbCpf1-NLS-3xHA
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mammalian codon-optimized Lachnospiraceae bacterium ND2006 Cpf1-NLS-3xHA
    Use
    CRISPR
    Tags
    NLS-3xHA
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    June 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    78744-plasmid-map-sequence-id-147455

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  24. Mutant ornithine transcarbamylase aa 30-114 deleted (OTC-delta)

    Plasmid
    #71878
    Purpose
    For mitochondrial unfolded protein response research
    Depositor
    Nicholas Hoogenraad
    Publication
    Zhao et al EMBO J. 2002 Sep 2;21(17):4411-9.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    OTC-delta (Otc Rat)
    Mutation
    Deleted amino acid 30-114 from full length OTC
    Available since
    Jan. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    71878-plasmid-map-sequence-id-135122

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  25. Mutant ornithine transcarbamylase aa 30-114 deleted (OTC-delta)

    Plasmid
    #71878
    Purpose
    For mitochondrial unfolded protein response research
    Depositor
    Nicholas Hoogenraad
    Publication
    Zhao et al EMBO J. 2002 Sep 2;21(17):4411-9.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    OTC-delta (Otc Rat)
    Mutation
    Deleted amino acid 30-114 from full length OTC
    Available since
    Jan. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    71878-plasmid-map-sequence-id-135122

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  32. pCRISPathBrick

    Plasmid
    #65006
    Purpose
    E. coli vector for expression of S. pyogenes dCas9, tracrRNA, and nontargeting CRISPR array with BsaI site for inserting user-defined spacer-repeat bricks
    Depositor
    Mattheos Koffas
    Publication
    Cress et al ACS Synth Biol. 2015 Mar 30.
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    Constitutive native promoters
    Available since
    May 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    7 Articles
    65006-plasmid-map-sequence-id-109903

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  33. pCRISPathBrick

    Plasmid
    #65006
    Purpose
    E. coli vector for expression of S. pyogenes dCas9, tracrRNA, and nontargeting CRISPR array with BsaI site for inserting user-defined spacer-repeat bricks
    Depositor
    Mattheos Koffas
    Publication
    Cress et al ACS Synth Biol. 2015 Mar 30.
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    Constitutive native promoters
    Available since
    May 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    7 Articles
    65006-plasmid-map-sequence-id-109903

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  38. PX856

    Plasmid
    #62888
    Purpose
    C-term SpCas9 piece of inducible transcriptional activator (dCas9(C)-FKBP-2xNLS-VP64)
    Depositor
    Feng Zhang
    Publication
    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9 (aa536-1368)
    Use
    CRISPR
    Tags
    FKBP12, NLS, NLS SV40, and VP64
    Expression
    Mammalian
    Mutation
    Asparagine 863 to Alanine (N863A)
    Promoter
    CBh
    Available since
    March 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    62888-plasmid-map-sequence-id-105911

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  39. PX856

    Plasmid
    #62888
    Purpose
    C-term SpCas9 piece of inducible transcriptional activator (dCas9(C)-FKBP-2xNLS-VP64)
    Depositor
    Feng Zhang
    Publication
    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9 (aa536-1368)
    Use
    CRISPR
    Tags
    FKBP12, NLS, NLS SV40, and VP64
    Expression
    Mammalian
    Mutation
    Asparagine 863 to Alanine (N863A)
    Promoter
    CBh
    Available since
    March 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    62888-plasmid-map-sequence-id-105911

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  48. mNeptune2-N1

    Plasmid
    #54837
    Purpose
    Localization: N1 Cloning Vector, Excitation: 600, Emission: 650
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    mNeptune2
    Expression
    Mammalian
    Available since
    June 11, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    1 Article
    54837-plasmid-map-sequence-id-162708

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  49. mNeptune2-N1

    Plasmid
    #54837
    Purpose
    Localization: N1 Cloning Vector, Excitation: 600, Emission: 650
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    mNeptune2
    Expression
    Mammalian
    Available since
    June 11, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    1 Article
    54837-plasmid-map-sequence-id-162708

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  50. pAAV-hSyn-DIO-HA-hM4D(Gi)-IRES-mCitrine

    Plasmid
    #50455
    Purpose
    Double floxed Gi-coupled hM4D-IRES-mCitrine under the control of human synapsin promoter
    Depositor
    Bryan Roth
    Has service
    AAV8
    Insert
    hM4D(Gi)-IRES-mCitrine (CHRM4 Human)
    Use
    AAV
    Tags
    HA
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    human Synapsin 1
    Available since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    20 Articles
    50455-plasmid-map-sequence-id-214301

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Showing: 9501 - 9550 of 291538 results