We narrowed to 30,799 results for: Ide
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NmCas9_sgRNA_expression_in_pBluescript
Plasmid#122091PurposeU6 driven NmCas9 sgRNA expression vector for cloning own guidesDepositorInsertNmCas9 tracrRNA (NEWENTRY Synthetic, S. pyogenes)
UseCRISPRExpressionMammalianPromoterU6Available SinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 MCS-BirA(R118G)-HA
Plasmid#36047PurposeTo fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); HA tagDepositorHas ServiceCloning Grade DNAInsertBirA(R118G)-HA
TagsHAExpressionMammalianMutationR118G (highly promiscuous form)Available SinceApril 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
TREER-Actin
Plasmid#208330PurposeTREER genetically-encoded tag introduced before actin; to be used with TinyERRE probe peptide.DepositorInsertTREER-Actin
TagsTREERExpressionMammalianAvailable SinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
TOMM20-PuRRRE
Plasmid#208329PurposePuRRRE genetically-encoded tag introduced after TOMM20; to be used with PunyEEER probe peptide.DepositorInsertTOMM20-PuRRRE
TagsPuRRREExpressionMammalianAvailable SinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pICH-nuc-roGFP2-ORP1
Plasmid#191758PurposeTi plasmid pICH86988 harbouring nuc-roGFP2-ORP1 for peroxide detection in the nuclei of plant cells.DepositorInsertnuc-roGFP2-ORP1
UseBinary agrobacterium ti plasmidExpressionPlantPromoterCaMV 35SAvailable SinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLIX403-hNOS2
Plasmid#110800Purposetet-inducible NOS2 expressionDepositorAvailable SinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pB025
Plasmid#183092PurposeExpresses FnCas12a in Bacteroides and used for genome editingDepositorInsertFnCas12a, gRNA, Promoter, HAB, TetR,
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterP1TDPGH023Available SinceAug. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHelper_ShCAST_sgRNA
Plasmid#127921PurposeExpresses ShCAST and an empty sgRNA scaffold. New targets can be added using Golden Gate assembly (LguI sites).DepositorInsertShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning (LguI sites)
Available SinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-dCas9-5xPlat2AflD
Plasmid#82560PurposeExpression vector for Cas9 peptide array (linker length: 22aa).DepositorInsertdCas9-5xPlat2AflD
ExpressionMammalianAvailable SinceSept. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a SnoopTag-MBP
Plasmid#72323PurposeBacterial expression of Maltose Binding Protein linked to SnoopTag, a peptide tag forming a spontaneous covalent bond with its protein partner Snoopcatcher.DepositorInsertpET28a SnoopTag-MBP
TagsN-terminal His6 tag. MBP is fused at the C-termin…ExpressionBacterialAvailable SinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEJS2700-CMV-Nme2-ABE8e-nt-E932R
Plasmid#228721PurposeExpresses N-terminal ABE8e-Nme2 nCas9 (D16A) base editor with E932R mutation in mammalian cellsDepositorInsertN-terminal ABE8e with Nme2 nCas9 (D16A) E932R
UseCRISPRExpressionMammalianMutationNme2-Cas9 D16A E932RPromoterCMVAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS2712-CMV-eNme2-C-ABE8e-i1-L10
Plasmid#228733PurposeExpresses domain inlaid eNme2-C nCas9 (D16A)-i1-ABE8e base editor with linker-10 in mammalian cellsDepositorInsertDomain inlaid eNme-C nCas9 (D16A)-i1-L10
UseCRISPRExpressionMammalianPromoterCMVAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS2701-CMV-Nme2Smu-ABE8e-nt-E932R
Plasmid#228722PurposeExpresses N-terminal ABE8e-Nme2Smu nCas9 (D16A) base editor with E932R mutation in mammalian cellsDepositorInsertN-terminal ABE8e with Nme2Smu nCas9 (D16A) E932R
UseCRISPRExpressionMammalianMutationNme2-Cas9, Smu PID, D16A E932RPromoterCMVAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS2702-CMV-iNme2-ABE8e-nt
Plasmid#228723PurposeExpresses N-terminal ABE8e-iNme2 nCas9 (D16A) base editor in mammalian cellsDepositorInsertN-terminal ABE8e with iNme2 nCas9 (D16A)
UseCRISPRExpressionMammalianMutationNme2-Cas9 D16A D844G E868K K870R D873A D911G K929…PromoterCMVAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDECKO_mCherry
Plasmid#78534PurposeBackbone to clone single or paired sgRNAsDepositorTypeEmpty backboneUseLentiviralExpressionMammalianPromoterU6 (for expressing sgRNA)Available SinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGSKU
Plasmid#72243PurposeContains the CORE cassette with convergent KlURA3 and KanMX4 markers and the I-SceI gene under the inducible GAL1 promoterDepositorInsertsKlURA3
kanMX4
I-SceI
ExpressionBacterialAvailable SinceJan. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEJS2164_Nme2-ABE8e-i7
Plasmid#201517PurposeMammalian expression of domain inlaid Nme2-ABE8e-i7DepositorInsertNme2-ABE8e-i7
ExpressionMammalianPromoterCMVAvailable SinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS2158_Nme2-ABE8e-i1
Plasmid#201510PurposeMammalian expression of domain inlaid Nme2-ABE8e-i1DepositorInsertNme2-ABE8e-i1
ExpressionMammalianPromoterCMVAvailable SinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
modRNAc0-Cas9-2A-Puro
Plasmid#172855PurposeIVT template plasmid for making modified Cas9Puro RNADepositorInsertCas9-2A-Puro
UseIvt template plasmidTags2A-PuroAvailable SinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only
