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We narrowed to 63,812 results for: cloning

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Showing: 3861 - 3880 of 63812 results

  2. pUS2-SH231

    Plasmid
    #115150
    Purpose
    Cas9-GFP, dual gRNA expression vector for targeted integration of repair templates into safe harbor 231 locus in chromosome 4
    Depositor
    Raymond Monnat, Michael Phelps
    Publication
    Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Dual SH231 gRNAs
    Use
    CRISPR
    Tags
    3xFLAG-SV40NLS, GFP, and Nucleoplasmin NLS
    Expression
    Mammalian
    Promoter
    U6, CBh
    Available since
    Oct. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    115150-plasmid-map-sequence-id-224718

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  3. pJ151-HDR

    Plasmid
    #113631
    Purpose
    Contains two recombination homology arms and loxP-flanked cassette encoding puromycin resistance gene, Venus fluorescence marker, thymidine kinase suicide gene, for scarless chromosomal engineering.
    Depositor
    Alex Kentsis
    Inserts
    Left homology arm
    Puromycin resistance gene
    Venus fluorescence gene
    Thymidine kinase
    Right homology arm
    Use
    Cre/Lox; Homology recombination selection vector
    Expression
    Bacterial and Mammalian
    Promoter
    EF1a promoter, EF1a promoter (T2A cleaving sequen…
    Available since
    Sept. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    113631-plasmid-map-sequence-id-222216

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  6. VinTS-tCRMod-GGSGGS9

    Plasmid
    #111765
    Purpose
    Expresses vinculin tension sensor comprised of Clover-(GGSGGS)9-mRuby2 module
    Depositor
    Brenton Hoffman
    Publication
    LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Clover-mRuby2-based vinculin tension sensor with (GGSGGS)9 extensible domain
    Expression
    Mammalian
    Mutation
    Residues 1-228 of Clover, residues 1-3 deleted fr…
    Promoter
    CMV
    Available since
    July 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    111765-plasmid-map-sequence-id-214720

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  7. pLenti Lifeact-mTagBFP2 PuroR

    Plasmid
    #101893
    Purpose
    Lentiviral expression of mTagBFP2-tagged Lifeact with Puromycin selection in cells including neurons
    Depositor
    Ghassan Mouneimne
    Insert
    Lifeact
    Use
    Lentiviral
    Tags
    mTagBFP2
    Promoter
    CMV immediate early
    Available since
    May 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    101893-plasmid-map-sequence-id-211159

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  9. pCSN068

    Plasmid
    #101749
    Purpose
    Single copy yeast plasmid expressing Cpf1 from Francisella novicida U112 (FnCpf1), codon optimized for expression in Saccharomyces cerevisiae.
    Depositor
    Rene Verwaal
    Publication
    Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278
    Inserts
    Cpf1 from Francisella novicida U112 (FnCpf1) codon optimized for expression in S. cerevisiae.
    KanMX marker expression cassette.
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Yeast
    Promoter
    Heterologous TEF1 promoter from A. gossypii. and …
    Available since
    Jan. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    101749-plasmid-map-sequence-id-202306

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  15. pRRlsinPGK_H2BGFP_WPRE

    Plasmid
    #91788
    Purpose
    Constitutive expression of H2BGFP fusion using PGK promoter in a lentiviral backbone.
    Depositor
    Beverly Torok-Storb
    Insert
    Human histone H2B (H2BC11 Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    PGK
    Available since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    91788-plasmid-map-sequence-id-180614

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Showing: 3861 - 3880 of 63812 results