We narrowed to 5,563 results for: cycs
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Plasmid#138230PurposeExpresses the split mCherry reporter interrupted by the Ama-Ter artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only
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pZH517
Plasmid#102670PurposeTetracycline inducible expression of GFPmut2 using autoregulation; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
ExpressionBacterialAvailable SinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFP.R371
Plasmid#138241PurposeExpresses the split mCherry reporter interrupted by the MP-M-DnaB artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHisIHFαIHFβ
Plasmid#149384PurposeExpression of P. aeruginosa PA14, 6xHis-HRV3C-IHFalpha and IHFbetaDepositorInsertsPseudomonas aeruginosa PA14 6xHis-tagged IHFalpha
Pseudomonas aeruginosa PA14 IHFbeta
Tags6xHis tag and HRV3C cleavage siteExpressionBacterialPromoterT7Available SinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLMB713
Plasmid#83785PurposeSalicylic acid biosensor plasmidDepositorInsertRegion upstream RL1329 (salA) containing all of RL1328 (salR)
Available SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSW002-PpsbA
Plasmid#108236PurposeBroad host bacterial expression vector with constitutive PsbA promoterDepositorInsertPsbA promoter
ExpressionBacterialAvailable SinceMay 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRL_yEGFP_hc
Plasmid#98746PurposeReporter plasmid for the PhiReX system. Encodes the reporter gene yEGFP under the control of the CYC1 minimal promoter with upstream synTALE-DBS.DepositorInsertYeast-enhanced green fluorescent protein
UseSynthetic BiologyExpressionYeastPromotersyntheticAvailable SinceAug. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCS4
Plasmid#55751PurposeProduces one half (alpha) of aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.DepositorInsertKanR
ExpressionBacterialMutationResidues 1-59 of KanRPromoterT7-lacAvailable SinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
FLAG-ADRB2-mNG
Plasmid#195145PurposeImage recycling cargoDepositorInsertADRB3 (ADRB3 Human)
ExpressionMammalianAvailable SinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
P303-FBP_2
Plasmid#139693PurposeT-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway, without recycling gene CPHDepositorInsertp35S_Short_TMV 5':AsNpga:tAtug7, pCmYLCV:NnH3H:AtuOCS, pCmYLCV:NnHisps:tHsp, p35S:NnLuz:tAtuOcs
UseLuciferaseExpressionPlantPromotervariousAvailable SinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKF-P14MM2AG5h
Plasmid#128254PurposeMammalian positive feedback synthetic gene circuit within a Flp-In expression vector.DepositorInsertrtTA-Advanced::P2A::EGFP
UseSynthetic Biology; Flp-in expression vectorExpressionMammalianPromotertight TRE promoterAvailable SinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRTS-GBP-dCas9-mRFP
Plasmid#102934PurposeTetracyclin inducible expression construct for dCas9 fused to GFP-binding Protein (N-terminally) and mRFP (C-terminus)DepositorInsertdCas9
UseCRISPRTagsGBP (GFP binding protein and mRFPExpressionMammalianAvailable SinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pASKt15C+SrtA5
Plasmid#64985PurposeExpresses the P94R/D160N/D165A/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
TagsHis6 tag and S tagExpressionBacterialMutationdeleted amino acids 1-59, P94R, D160N, D165A, K19…Promotertetracycline promoter/operatorAvailable SinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFUW-tetO-mOrange
Plasmid#134298Purposedoxycycline-inducible expression of mouse mOrange in mammalian cellsDepositorInsertmOrange
UseLentiviralExpressionMammalianAvailable SinceDec. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZH519
Plasmid#102672PurposeTetracycline inducible expression of GFPmut2 using autoregulation; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
ExpressionBacterialAvailable SinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFA-LEU2-I-SceI
Plasmid#67595PurposeI-SceI expression regulated by tetracycline inducible system in S. pombeDepositorInsertsnmt terminator
I-SceI
TetR
UseGene targeting in yeastAvailable SinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZH522
Plasmid#102675PurposeTetracycline inducible expression of GFPmut2 with constitutive TetR; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
ExpressionBacterialAvailable SinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAF256
Plasmid#105496PurposepRPF185 derived plasmid encoding an anhydrotetracyclin inducible HupA-SmBiT and LgBiTDepositorInsertHupA-SmBiT/LgBiT
UseAtc-dependent expression in c. difficilePromoterPtetAvailable SinceSept. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHSP70B-CP6-TK
Plasmid#165881PurposeBasic ganciclovir or acyclovir sensitvity cassette in Alpha2, lacks physical marker or attB siteDepositorInsertBasic Selection Vector
UseSynthetic BiologyAvailable SinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAV0714
Plasmid#133479PurposeExpression in fission yeast from promoter pact1, integrated at ura4 locusDepositorInsertpUra4(AfeI)-p(act1)-sfGFP-terminator(ScCYC1)
ExpressionYeastAvailable SinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only