We narrowed to 8,748 results for: SARS

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pET-28a_6H-TAQ_E602D

Plasmid

#166944

Purpose

Bacterial expression of Taq DNA polymerase

Depositor

Robert Tjian

Article

Graham et al PLoS One. 2021 Feb 3;16(2):e0246647. doi

Insert

Thermus aquaticus DNA polymerase

Tags

6X His

Expression

Bacterial

Mutation

E602D

Promoter

T7

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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FASTHDR-Cterm-mClover3-Puromycin

Plasmid

#167205

Purpose

Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mClover3 and selection with Puromycin

Depositor

Oscar Perez

Article

Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

Type

Empty backbone

Use

CRISPR

Tags

mClover3

Expression

Mammalian

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3-SREBP-2-GFP

Plasmid

#204202

Purpose

Mammalian expression of SREBP-2

Depositor

Allan Weissman

Article

Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

Insert

SREBP-2 (SREBF2 Human)

Tags

T7x3 and eGFP

Expression

Mammalian

Mutation

multiple silent mutations

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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AttB_ACE2[R.alcyone+ferrum]-2A-TMPRSS2_IRES-iRFP670-P2A-PuroR

Plasmid

#190079

Purpose

express chimeric Rhinolophus alcyone ACE2 and human TMPRSS2 with selectable markers iRFP670 and puromycin resistance gene

Depositor

Kenneth Matreyek

Article

Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

Insert

ACE2 (TMPRSS2 Synthetic, Rhinolophus alcyone and Rhinolophus ferrumequinum)

Expression

Mammalian

Promoter

none

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hACE2

Plasmid

#145033

Purpose

Express human ACE2 with C9 tag at C-terminal in mammalian cells

Depositor

Fang Li

Article

Shang et al Nature. 2020 Mar 30. pii: 10.1038/s41586

Insert

human ACE2 (ACE2 Human)

Tags

C9

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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FASTHDR-Cterm-mRuby3-Zeo

Plasmid

#167206

Purpose

Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mRuby3 and selection with Zeocin

Depositor

Oscar Perez

Article

Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

Type

Empty backbone

Use

CRISPR

Tags

mRuby3

Expression

Mammalian

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-J chain

Plasmid

#145146

Purpose

Mammalian expression plasmid for J chain of polymeric antibody classes

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

immunoglobulin J chain (JCHAIN Human)

Expression

Mammalian

Mutation

Codon-optimized for human cell expression.

Promoter

CMV

Available Since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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FASTHDR-Cterm-HaloTag-Puromycin

Plasmid

#167208

Purpose

Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with HaloTag and selection with Puromycin

Depositor

Oscar Perez

Article

Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

Type

Empty backbone

Use

CRISPR

Tags

HaloTag

Expression

Mammalian

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hACE2-LgBiT

Plasmid

#215837

Purpose

Expresses hACE2 receptor with LgBiT tag in mammalian cells

Depositor

Thomas Gallagher

Article

Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

Insert

hACE2-LgBiT (ACE2 )

Expression

Mammalian

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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FASTHDR-Cterm-NanoLuc-Puromycin

Plasmid

#167209

Purpose

Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with NanoLuc and selection with Puromycin

Depositor

Oscar Perez

Article

Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

Type

Empty backbone

Use

CRISPR

Tags

NanoLuc

Expression

Mammalian

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pHR_Gal4UASpyb-TATA_tBFP_pGK_mCitrine (RL407)

Plasmid

#194217

Purpose

Lentiviral transducition vector for a GAL4-responsive tagBFP reporter gene

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv [Preprint]. 2021 Apr 20:2021.04.

Insert

UAS-ybTATA-tagBFP; PGK-mCitrine

Use

Lentiviral

Promoter

GAL4 UAS-ybTATA; PGK

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-mScarlet-Control-5'UTR

Plasmid

#184637

Purpose

Fluorescent reporter for mammalian cells: Expression of mScarlet with a CMV 5'UTR

Depositor

Hao Wu

Article

Vora et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

Insert

mScarlet

Use

Lentiviral

Expression

Mammalian

Available Since

June 2, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV-hARdeltaNH2-6

Plasmid

#89104

Purpose

mammalian expression of human androgen receptor amino acids 507-919

Depositor

Elizabeth Wilson

Article

Simental et al J Biol Chem. 1991 Jan 5;266(1):510-8.

Insert

hAR DNA binding domain+ligand binding domain (AR Human)

Expression

Mammalian

Mutation

NH2 terminal deletion, contains amino acids 507-…

Promoter

CMV

Available Since

April 18, 2017

Availability

Academic Institutions and Nonprofits only

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FASTHDR-Cterm-mTagBFP2-Blasticidin

Plasmid

#167207

Purpose

Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mTagBFP2 and selection with Blasticidin

Depositor

Oscar Perez

Article

Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

Type

Empty backbone

Use

CRISPR

Tags

mTagBFP2

Expression

Mammalian

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-hAR1-660

Plasmid

#89079

Purpose

mammalian expression of human androgen receptor N-terminal fragment 1-660 amino acids

Depositor

Elizabeth Wilson

Article

Simental et al J Biol Chem. 1991 Jan 5;266(1):510-8.

Insert

hAR-1-660 (AR Human)

Expression

Mammalian

Mutation

aa 1-660 (contains exons A,B,C and part of D)

Promoter

CMV

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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IDE-2Strep

Plasmid

#210355

Purpose

Expresses IDE fused with 2Strep in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

IDE (IDE Human)

Tags

2Strep

Expression

Mammalian

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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MTK Lentivirus Multi Cassette Destination Vector (pAN2424)

Plasmid

#194221

Purpose

Backbone for constructing multi casette expression plasmids using the Mammalian Tookit

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv [Preprint]. 2021 Apr 20:2021.04.

Type

Empty backbone

Use

Lentiviral

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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eGFP-HA_BASU

Plasmid

#153996

Purpose

Express eGFP gene along with HA and BASU

Depositor

Paul Khavari

Article

Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

Insert

E-GFP

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-LYRa11-RBD-8h

Plasmid

#161822

Purpose

Mammalian expression plasmid for RBD of bat coronavirus isolate LYRa11

Depositor

Erik Procko

Article

Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

Insert

Receptor-binding domain (RBD) of spike protein S

Tags

GSG linker and 8his tag and HA leader sequence

Expression

Mammalian

Mutation

Human codon optimized RBD (a.a. T324-D522)

Promoter

CMV

Available Since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RsSHC014-RBD-8h

Plasmid

#161826

Purpose

Mammalian expression plasmid for RBD of bat coronavirus isolate RsSHC014

Depositor

Erik Procko

Article

Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

Insert

Receptor-binding domain (RBD) of spike protein S

Tags

GSG linker and 8his tag and HA leader sequence

Expression

Mammalian

Mutation

Human codon optimized RBD (a.a. T321-D519)

Promoter

CMV

Available Since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

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